No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95828
Title:
No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.
Authors:
Williams, K J; Heighway, Jim; Birch, Jillian M; Norton, John D; Scott, David
Abstract:
The radiation response of Epstein-Barr virus (EBV)-immortalised lymphoblastoid cell lines derive from Li-Fraumeni syndrome (LFS) and LFS-like individuals was investigated. Cells from all LFS and LFS-like cases showed an accumulation of p53 protein following 137Cs gamma-irradiation, which was associated with cell cycle arrest at the G1/S border. This response was indistinguishable from that seen in cells derived from normal individuals, and occurred in cases with missense mutations in the TP53 gene at codons 175, 180, 220 and 248 and also in two LFS-like individuals with no TP53 mutation. Previous studies using lymphocytes and fibroblasts from LFS individuals have demonstrated abnormal radiation responses in these cells. This suggest cell type specificity in the contribution of a mutant p53 protein to phenotype.
Affiliation:
CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines. 1996, 74 (5):698-703 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Sep-1996
URI:
http://hdl.handle.net/10541/95828
PubMed ID:
8795570
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWilliams, K Jen
dc.contributor.authorHeighway, Jimen
dc.contributor.authorBirch, Jillian Men
dc.contributor.authorNorton, John Den
dc.contributor.authorScott, Daviden
dc.date.accessioned2010-04-07T10:45:08Z-
dc.date.available2010-04-07T10:45:08Z-
dc.date.issued1996-09-
dc.identifier.citationNo defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines. 1996, 74 (5):698-703 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid8795570-
dc.identifier.urihttp://hdl.handle.net/10541/95828-
dc.description.abstractThe radiation response of Epstein-Barr virus (EBV)-immortalised lymphoblastoid cell lines derive from Li-Fraumeni syndrome (LFS) and LFS-like individuals was investigated. Cells from all LFS and LFS-like cases showed an accumulation of p53 protein following 137Cs gamma-irradiation, which was associated with cell cycle arrest at the G1/S border. This response was indistinguishable from that seen in cells derived from normal individuals, and occurred in cases with missense mutations in the TP53 gene at codons 175, 180, 220 and 248 and also in two LFS-like individuals with no TP53 mutation. Previous studies using lymphocytes and fibroblasts from LFS individuals have demonstrated abnormal radiation responses in these cells. This suggest cell type specificity in the contribution of a mutant p53 protein to phenotype.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshBurkitt Lymphoma-
dc.subject.meshDNA Damage-
dc.subject.meshFemale-
dc.subject.meshFlow Cytometry-
dc.subject.meshG1 Phase-
dc.subject.meshGamma Rays-
dc.subject.meshHumans-
dc.subject.meshImmunoblotting-
dc.subject.meshLi-Fraumeni Syndrome-
dc.subject.meshLymphocytes-
dc.subject.meshMale-
dc.subject.meshS Phase-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshTumor Suppressor Protein p53-
dc.titleNo defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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