DNA inclusions within autolytic cytoplasmic vacuoles of hemopoietic stem cell line FDCP-Mix.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95815
Title:
DNA inclusions within autolytic cytoplasmic vacuoles of hemopoietic stem cell line FDCP-Mix.
Authors:
Reipert, Siegfried; Hickman, John A; Allen, Terence D
Abstract:
FDCP-Mix, a pluripotent routine hemopoietic stem cell line undergoes internucleosomal cleavage of DNA when induced to apoptosis either by drugs or by withdrawal of growth factor (IL-3), and also displays a pattern of nuclear morphology that is typical for apoptosis. However, increased autolytic activity in the cytoplasm precedes the nuclear changes. For etoposide-treated FDCP-Mix cells, mitochondria were identified as a target for autolytic digestion in large autolytic vacuoles, but during this period an increase in the number of mitochondria was observed. The autolytic vacuoles displayed variations in their content. Large, electron-dense inclusions resembling "condensed chromatin" could regularly be found in FDCP-Mix cells treated with low concentrations of etoposide (<4 microM). Confocal fluorescence microscopy and DNAse-gold labeling were employed to demonstrate the presence of DNA in the formation of the electron-dense inclusions within autolytic vacuoles. The identification of mitochondrial macroautophagy, the evidence for an etoposide-induced proliferation of mitochondria, and the fact that electron-dense inclusions are formed at a stage when the morphology of the nucleus is still not effected, suggests that the DNA within the autolytic vacuoles may be of mitochondrial origin.
Affiliation:
CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Citation:
DNA inclusions within autolytic cytoplasmic vacuoles of hemopoietic stem cell line FDCP-Mix. 1996, 44 (6):549-58 J. Histochem. Cytochem.
Journal:
The Journal of Histochemistry and Cytochemistry
Issue Date:
Jun-1996
URI:
http://hdl.handle.net/10541/95815
PubMed ID:
8666740
Type:
Article
Language:
en
ISSN:
0022-1554
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorReipert, Siegfrieden
dc.contributor.authorHickman, John Aen
dc.contributor.authorAllen, Terence Den
dc.date.accessioned2010-04-07T08:27:21Z-
dc.date.available2010-04-07T08:27:21Z-
dc.date.issued1996-06-
dc.identifier.citationDNA inclusions within autolytic cytoplasmic vacuoles of hemopoietic stem cell line FDCP-Mix. 1996, 44 (6):549-58 J. Histochem. Cytochem.en
dc.identifier.issn0022-1554-
dc.identifier.pmid8666740-
dc.identifier.urihttp://hdl.handle.net/10541/95815-
dc.description.abstractFDCP-Mix, a pluripotent routine hemopoietic stem cell line undergoes internucleosomal cleavage of DNA when induced to apoptosis either by drugs or by withdrawal of growth factor (IL-3), and also displays a pattern of nuclear morphology that is typical for apoptosis. However, increased autolytic activity in the cytoplasm precedes the nuclear changes. For etoposide-treated FDCP-Mix cells, mitochondria were identified as a target for autolytic digestion in large autolytic vacuoles, but during this period an increase in the number of mitochondria was observed. The autolytic vacuoles displayed variations in their content. Large, electron-dense inclusions resembling "condensed chromatin" could regularly be found in FDCP-Mix cells treated with low concentrations of etoposide (<4 microM). Confocal fluorescence microscopy and DNAse-gold labeling were employed to demonstrate the presence of DNA in the formation of the electron-dense inclusions within autolytic vacuoles. The identification of mitochondrial macroautophagy, the evidence for an etoposide-induced proliferation of mitochondria, and the fact that electron-dense inclusions are formed at a stage when the morphology of the nucleus is still not effected, suggests that the DNA within the autolytic vacuoles may be of mitochondrial origin.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshAutolysis-
dc.subject.meshCell Line-
dc.subject.meshDNA-
dc.subject.meshDeoxyribonuclease I-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInclusion Bodies-
dc.subject.meshMice-
dc.subject.meshMitochondria-
dc.subject.meshVacuoles-
dc.titleDNA inclusions within autolytic cytoplasmic vacuoles of hemopoietic stem cell line FDCP-Mix.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalThe Journal of Histochemistry and Cytochemistryen

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