Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95798
Title:
Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours.
Authors:
Andrews, P W; Casper, J; Damjanov, I; Duggan-Keen, Margaret F; Giwercman, A; Hata, J; Von Keitz, A; Looijenga, L H; Millán, J L; Oosterhuis, J W; Pera, M; Sawada, M; Schmoll, H J; Skakkebaek, N E; Van Putten, W; Stern, Peter L
Abstract:
The pattern of cell surface antigen expression of a set of cell lines derived from human germ cell tumours and corresponding to various cell phenotypes found within these tumours was studied using immunofluorescence. Twenty-two different antibodies were used. Many of these antibodies have been noted to recognise epitopes that are either preferentially expressed by embryonal carcinoma (EC) cells, or by more differentiated cell types. Using scatter plots and rank correlations, 6 groups of antibodies were distinguished with respect to their staining patterns on the cell lines tested. Several antibodies showed a specific staining pattern in relation to the differentiation state of the cells. Two groups of antibodies included those recognising high m.w. glycoproteins (antibodies TRA-1-60, TRA-1-81, GCTM2, 3-177, K4 and K21) and the ganglioseries glycolipid antigens SSEA-3 and -4 (antibodies MC631 and MC813-70). These antibodies mostly stained EC cells but not other cell types, confirming previously published data. However, one of these groups, comprising antibodies K4 and MC631, was more exclusively associated with the EC cell phenotype than was the other group. Antibodies recognising the liver isozyme of alkaline phosphatase (TRA-2-49 and TRA-2-54) also reacted strongly with most EC cell lines, although they reacted significantly with a number of other cell lines as well, whereas antibodies to the placental isozyme tended to react only weakly with EC cells. The antibodies recognising the ganglioseries glycolipids GD2 and GD3 (VIN2PB22 and VINIS56) preferentially stained cells with neuroectodermal characteristics. Other antibodies showed a heterogeneous staining pattern for the cell lines with different phenotypes. The data obtained from the cell lines were, in general, similar to data obtained from immunohistochemical studies on tissue sections of primary germ cell tumours of the adult testis, including carcinoma in situ.
Affiliation:
Department of Biomedical Science, University of Sheffield, Sheffield, UK.
Citation:
Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours. 1996, 66 (6):806-16 Int. J. Cancer
Journal:
International Journal of Cancer
Issue Date:
11-Jun-1996
URI:
http://hdl.handle.net/10541/95798
DOI:
10.1002/(SICI)1097-0215(19960611)66:6<806::AID-IJC17>3.0.CO;2-0
PubMed ID:
8647654
Type:
Article
Language:
en
ISSN:
0020-7136
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorAndrews, P Wen
dc.contributor.authorCasper, Jen
dc.contributor.authorDamjanov, Ien
dc.contributor.authorDuggan-Keen, Margaret Fen
dc.contributor.authorGiwercman, Aen
dc.contributor.authorHata, Jen
dc.contributor.authorVon Keitz, Aen
dc.contributor.authorLooijenga, L Hen
dc.contributor.authorMillán, J Len
dc.contributor.authorOosterhuis, J Wen
dc.contributor.authorPera, Men
dc.contributor.authorSawada, Men
dc.contributor.authorSchmoll, H Jen
dc.contributor.authorSkakkebaek, N Een
dc.contributor.authorVan Putten, Wen
dc.contributor.authorStern, Peter Len
dc.date.accessioned2010-04-07T08:19:19Z-
dc.date.available2010-04-07T08:19:19Z-
dc.date.issued1996-06-11-
dc.identifier.citationComparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours. 1996, 66 (6):806-16 Int. J. Canceren
dc.identifier.issn0020-7136-
dc.identifier.pmid8647654-
dc.identifier.doi10.1002/(SICI)1097-0215(19960611)66:6<806::AID-IJC17>3.0.CO;2-0-
dc.identifier.urihttp://hdl.handle.net/10541/95798-
dc.description.abstractThe pattern of cell surface antigen expression of a set of cell lines derived from human germ cell tumours and corresponding to various cell phenotypes found within these tumours was studied using immunofluorescence. Twenty-two different antibodies were used. Many of these antibodies have been noted to recognise epitopes that are either preferentially expressed by embryonal carcinoma (EC) cells, or by more differentiated cell types. Using scatter plots and rank correlations, 6 groups of antibodies were distinguished with respect to their staining patterns on the cell lines tested. Several antibodies showed a specific staining pattern in relation to the differentiation state of the cells. Two groups of antibodies included those recognising high m.w. glycoproteins (antibodies TRA-1-60, TRA-1-81, GCTM2, 3-177, K4 and K21) and the ganglioseries glycolipid antigens SSEA-3 and -4 (antibodies MC631 and MC813-70). These antibodies mostly stained EC cells but not other cell types, confirming previously published data. However, one of these groups, comprising antibodies K4 and MC631, was more exclusively associated with the EC cell phenotype than was the other group. Antibodies recognising the liver isozyme of alkaline phosphatase (TRA-2-49 and TRA-2-54) also reacted strongly with most EC cell lines, although they reacted significantly with a number of other cell lines as well, whereas antibodies to the placental isozyme tended to react only weakly with EC cells. The antibodies recognising the ganglioseries glycolipids GD2 and GD3 (VIN2PB22 and VINIS56) preferentially stained cells with neuroectodermal characteristics. Other antibodies showed a heterogeneous staining pattern for the cell lines with different phenotypes. The data obtained from the cell lines were, in general, similar to data obtained from immunohistochemical studies on tissue sections of primary germ cell tumours of the adult testis, including carcinoma in situ.en
dc.language.isoenen
dc.subjectCancer Antigensen
dc.subjectCancer Proteinsen
dc.subjectTesticular Canceren
dc.subjectEndodermal Sinus Tumouren
dc.subjectCultured Tumour Cellsen
dc.subjectBiological Tumour Markersen
dc.subject.meshAdult-
dc.subject.meshAlkaline Phosphatase-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshAntibodies, Neoplasm-
dc.subject.meshAntibody Specificity-
dc.subject.meshAntigens, Neoplasm-
dc.subject.meshAntigens, Surface-
dc.subject.meshBiopsy-
dc.subject.meshCarcinoma in Situ-
dc.subject.meshCarcinoma, Embryonal-
dc.subject.meshEndodermal Sinus Tumor-
dc.subject.meshFlow Cytometry-
dc.subject.meshFluorescent Antibody Technique, Indirect-
dc.subject.meshGene Expression-
dc.subject.meshGerminoma-
dc.subject.meshGlycolipids-
dc.subject.meshHumans-
dc.subject.meshImmunophenotyping-
dc.subject.meshIsoenzymes-
dc.subject.meshMale-
dc.subject.meshMembrane Glycoproteins-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshSeminoma-
dc.subject.meshTesticular Neoplasms-
dc.subject.meshTestis-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshTumor Markers, Biological-
dc.titleComparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biomedical Science, University of Sheffield, Sheffield, UK.en
dc.identifier.journalInternational Journal of Canceren

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.