Evaluation of cytogenetic conversion to Ph- haemopoiesis in long-term bone marrow culture for patients with chronic myeloid leukaemia on conventional hydroxyurea therapy, on pulse high-dose hydroxyurea and on interferon-alpha.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95703
Title:
Evaluation of cytogenetic conversion to Ph- haemopoiesis in long-term bone marrow culture for patients with chronic myeloid leukaemia on conventional hydroxyurea therapy, on pulse high-dose hydroxyurea and on interferon-alpha.
Authors:
Coutinho, Lucia H; Brereton, M L; Santos, A M; Ryder, W David J; Chang, James; Harrison, Christine J; Yin, J A; Dexter, T Michael; Testa, Nydia G
Abstract:
Long-term bone marrow culture (LTBMC) has been used successfully in autologous transplantation in chronic myeloid leukaemia (CML). However, variation between patients in the recovery of Ph- cells in culture limits the application of this procedure to a minority. Treatment that effectively reduces in vivo tumour burden prior to initiation of LTBMC may improve the selection of Ph- cells in culture. To test this hypothesis we evaluated the frequency and degree of cytogenetic conversion to Ph- haemopoiesis in LTBMC from four independent groups of CML patients: Untreated (n = 19); conventional dosage of hydroxyurea (HU) (n = 10); pulse high-dose HU (P-HU) (n = 22) and interferon (IFN)-alpha (n = 12). In this study IFN-alpha therapy resulted in a significantly higher incidence of patients with detectable Ph- clonogenic cells in the marrow (P = 0.01) and with > or = 50% Ph- haemopoiesis in LTBMC as compared to newly diagnosed patients (P = 0.05). Also, sequential culture studies undertaken in 14 CML patients at diagnosis and following the start of pulse highdose HU therapy showed that in eight patients the average proportion of Ph- metaphases detected in LTBMC substantially increased from 1.7% (range 0-7) at diagnosis to levels of 71% (range 14-100) after treatment. Therefore we conclude that the use of IFN or pulse high-dose HU in early stage disease appears to create an opportunity to harvest the marrow for long-term culture (LTC) purging with reduced leukaaemic burden.
Affiliation:
CRC Department of Experimental Haematology, Christie Hospital NHS Trust, Manchester.
Citation:
Evaluation of cytogenetic conversion to Ph- haemopoiesis in long-term bone marrow culture for patients with chronic myeloid leukaemia on conventional hydroxyurea therapy, on pulse high-dose hydroxyurea and on interferon-alpha. 1996, 93 (4):869-77 Br. J. Haematol.
Journal:
British Journal of Haematology
Issue Date:
Jun-1996
URI:
http://hdl.handle.net/10541/95703
DOI:
10.1046/j.1365-2141.1996.d01-1733.x
PubMed ID:
8703819
Type:
Article
Language:
en
ISSN:
0007-1048
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorCoutinho, Lucia Hen
dc.contributor.authorBrereton, M Len
dc.contributor.authorSantos, A Men
dc.contributor.authorRyder, W David Jen
dc.contributor.authorChang, Jamesen
dc.contributor.authorHarrison, Christine Jen
dc.contributor.authorYin, J Aen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-04-06T11:47:39Z-
dc.date.available2010-04-06T11:47:39Z-
dc.date.issued1996-06-
dc.identifier.citationEvaluation of cytogenetic conversion to Ph- haemopoiesis in long-term bone marrow culture for patients with chronic myeloid leukaemia on conventional hydroxyurea therapy, on pulse high-dose hydroxyurea and on interferon-alpha. 1996, 93 (4):869-77 Br. J. Haematol.en
dc.identifier.issn0007-1048-
dc.identifier.pmid8703819-
dc.identifier.doi10.1046/j.1365-2141.1996.d01-1733.x-
dc.identifier.urihttp://hdl.handle.net/10541/95703-
dc.description.abstractLong-term bone marrow culture (LTBMC) has been used successfully in autologous transplantation in chronic myeloid leukaemia (CML). However, variation between patients in the recovery of Ph- cells in culture limits the application of this procedure to a minority. Treatment that effectively reduces in vivo tumour burden prior to initiation of LTBMC may improve the selection of Ph- cells in culture. To test this hypothesis we evaluated the frequency and degree of cytogenetic conversion to Ph- haemopoiesis in LTBMC from four independent groups of CML patients: Untreated (n = 19); conventional dosage of hydroxyurea (HU) (n = 10); pulse high-dose HU (P-HU) (n = 22) and interferon (IFN)-alpha (n = 12). In this study IFN-alpha therapy resulted in a significantly higher incidence of patients with detectable Ph- clonogenic cells in the marrow (P = 0.01) and with > or = 50% Ph- haemopoiesis in LTBMC as compared to newly diagnosed patients (P = 0.05). Also, sequential culture studies undertaken in 14 CML patients at diagnosis and following the start of pulse highdose HU therapy showed that in eight patients the average proportion of Ph- metaphases detected in LTBMC substantially increased from 1.7% (range 0-7) at diagnosis to levels of 71% (range 14-100) after treatment. Therefore we conclude that the use of IFN or pulse high-dose HU in early stage disease appears to create an opportunity to harvest the marrow for long-term culture (LTC) purging with reduced leukaaemic burden.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectLeukaemiaen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshBone Marrow-
dc.subject.meshFemale-
dc.subject.meshHematopoiesis-
dc.subject.meshHumans-
dc.subject.meshHydroxyurea-
dc.subject.meshInterferon-alpha-
dc.subject.meshLeukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshTumor Cells, Cultured-
dc.titleEvaluation of cytogenetic conversion to Ph- haemopoiesis in long-term bone marrow culture for patients with chronic myeloid leukaemia on conventional hydroxyurea therapy, on pulse high-dose hydroxyurea and on interferon-alpha.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Experimental Haematology, Christie Hospital NHS Trust, Manchester.en
dc.identifier.journalBritish Journal of Haematologyen

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