Evidence for reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95657
Title:
Evidence for reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas.
Authors:
Watson, A J; Merritt, A J; Jones, L S; Askew, J N; Anderson, Elizabeth; Becciolini, A; Balzi, M; Potten, Christopher S; Hickman, John A
Abstract:
Evidence of accumulating for the failure of apoptosis as an important factor in the evolution of colorectal cancer and its poor response to adjuvant therapy. The proto-oncogene bcl-2 suppresses apoptosis. Its expression could provide an important survival advantage permitting the development of colorectal cancer. The expression of bcl-2 and p53 was determined by immunohistochemistry in 47 samples of histologically normal colonic mucosa, 19 adenomas and 53 adenocarcinomas. Expression of bcl-2 in colonic crypts > 5 cm from the tumours was confined to crypt bases but was more extensive and intense in normal crypts < 5 mm from cancers. A higher proportion of adenomas (63.2%) than carcinomas (36.5%) expressed bcl-2 (P < 0.05). A lower proportion of adenomas (31.6%) than carcinomas (62.3%) expressed p53 (P < 0.02). A total of 26.3% of adenomas and 22% of carcinomas expressed both bcl-2 and p53. To determine whether these samples contained cells which expressed both proteins, a dual staining technique for bcl-2 and p53 was used. Only 1/19 adenomas and 2/53 carcinomas contained cells immunopositive for both bcl-2 and p53. Moreover there was evidence of reciprocity of expression of bcl-2 and p53 in these three double staining neoplasms. We suggest that bcl-2 provides a survival advantage in the proliferative compartment of normal crypts and colorectal neoplasms. However, its expression is lost during the evolution from adenoma to carcinoma, whereas p53 expression is increased, an event generally coincident with the expression of stabilised p53, which we presume to represent the mutant form.
Affiliation:
Department of Medicine, University of Manchester, Hope Hospital, Salford, UK.
Citation:
Evidence for reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas. 1996, 73 (8):889-95 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Apr-1996
URI:
http://hdl.handle.net/10541/95657
PubMed ID:
8611422
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWatson, A Jen
dc.contributor.authorMerritt, A Jen
dc.contributor.authorJones, L Sen
dc.contributor.authorAskew, J Nen
dc.contributor.authorAnderson, Elizabethen
dc.contributor.authorBecciolini, Aen
dc.contributor.authorBalzi, Men
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorHickman, John Aen
dc.date.accessioned2010-04-06T10:41:07Z-
dc.date.available2010-04-06T10:41:07Z-
dc.date.issued1996-04-
dc.identifier.citationEvidence for reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas. 1996, 73 (8):889-95 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid8611422-
dc.identifier.urihttp://hdl.handle.net/10541/95657-
dc.description.abstractEvidence of accumulating for the failure of apoptosis as an important factor in the evolution of colorectal cancer and its poor response to adjuvant therapy. The proto-oncogene bcl-2 suppresses apoptosis. Its expression could provide an important survival advantage permitting the development of colorectal cancer. The expression of bcl-2 and p53 was determined by immunohistochemistry in 47 samples of histologically normal colonic mucosa, 19 adenomas and 53 adenocarcinomas. Expression of bcl-2 in colonic crypts > 5 cm from the tumours was confined to crypt bases but was more extensive and intense in normal crypts < 5 mm from cancers. A higher proportion of adenomas (63.2%) than carcinomas (36.5%) expressed bcl-2 (P < 0.05). A lower proportion of adenomas (31.6%) than carcinomas (62.3%) expressed p53 (P < 0.02). A total of 26.3% of adenomas and 22% of carcinomas expressed both bcl-2 and p53. To determine whether these samples contained cells which expressed both proteins, a dual staining technique for bcl-2 and p53 was used. Only 1/19 adenomas and 2/53 carcinomas contained cells immunopositive for both bcl-2 and p53. Moreover there was evidence of reciprocity of expression of bcl-2 and p53 in these three double staining neoplasms. We suggest that bcl-2 provides a survival advantage in the proliferative compartment of normal crypts and colorectal neoplasms. However, its expression is lost during the evolution from adenoma to carcinoma, whereas p53 expression is increased, an event generally coincident with the expression of stabilised p53, which we presume to represent the mutant form.en
dc.language.isoenen
dc.subjectColorectal Canceren
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshAdenocarcinoma-
dc.subject.meshAdenoma-
dc.subject.meshColorectal Neoplasms-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshProto-Oncogene Proteins-
dc.subject.meshProto-Oncogene Proteins c-bcl-2-
dc.subject.meshTumor Suppressor Protein p53-
dc.titleEvidence for reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine, University of Manchester, Hope Hospital, Salford, UK.en
dc.identifier.journalBritish Journal of Canceren

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