The quantitation and kinetics of unscheduled (repair) DNA synthesis in ultraviolet-irradiated human skin by automated image analysis.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95624
Title:
The quantitation and kinetics of unscheduled (repair) DNA synthesis in ultraviolet-irradiated human skin by automated image analysis.
Authors:
Chadwick, Caroline A; Diggle, S P; Young, A R; Potten, Christopher S
Abstract:
Grain counting by eye is a tedious and time-consuming technique but one with great potential in cell kinetics and for the study of DNA excision repair activity (unscheduled DNA synthesis or UDS). We have been investigating the levels of UDS in human skin sections exposed in situ to ultraviolet radiation using a short-term incubation in tritiated thymidine and autoradiography and the decline in UDS levels with time (repair kinetics). We have adapted an automated image analysis system automatically to assess the number of grains over epidermal cell nuclei in autoradiographs of sections of epidermis. An excellent correlation was observed between visual counting and machine measurement of the area (in pixels) occupied by silver grains. The levels of UDS declined with time as lesions are progressively repaired. The half time (+/- standard deviation) for the reduction in UDS is 7.25 +/- 0.18 h. The grain counts can be significantly increased by increasing the autoradiographic exposure, by increasing the concentration of tritiated thymidine and by increasing the incubation time.
Affiliation:
CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
Citation:
The quantitation and kinetics of unscheduled (repair) DNA synthesis in ultraviolet-irradiated human skin by automated image analysis. 1996, 135 (4):516-22 Br. J. Dermatol.
Journal:
The British Journal of Dermatology
Issue Date:
Oct-1996
URI:
http://hdl.handle.net/10541/95624
DOI:
10.1046/j.1365-2133.1996.d01-1032.x
PubMed ID:
8915138
Type:
Article
Language:
en
ISSN:
0007-0963
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorChadwick, Caroline Aen
dc.contributor.authorDiggle, S Pen
dc.contributor.authorYoung, A Ren
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-04-06T09:47:11Z-
dc.date.available2010-04-06T09:47:11Z-
dc.date.issued1996-10-
dc.identifier.citationThe quantitation and kinetics of unscheduled (repair) DNA synthesis in ultraviolet-irradiated human skin by automated image analysis. 1996, 135 (4):516-22 Br. J. Dermatol.en
dc.identifier.issn0007-0963-
dc.identifier.pmid8915138-
dc.identifier.doi10.1046/j.1365-2133.1996.d01-1032.x-
dc.identifier.urihttp://hdl.handle.net/10541/95624-
dc.description.abstractGrain counting by eye is a tedious and time-consuming technique but one with great potential in cell kinetics and for the study of DNA excision repair activity (unscheduled DNA synthesis or UDS). We have been investigating the levels of UDS in human skin sections exposed in situ to ultraviolet radiation using a short-term incubation in tritiated thymidine and autoradiography and the decline in UDS levels with time (repair kinetics). We have adapted an automated image analysis system automatically to assess the number of grains over epidermal cell nuclei in autoradiographs of sections of epidermis. An excellent correlation was observed between visual counting and machine measurement of the area (in pixels) occupied by silver grains. The levels of UDS declined with time as lesions are progressively repaired. The half time (+/- standard deviation) for the reduction in UDS is 7.25 +/- 0.18 h. The grain counts can be significantly increased by increasing the autoradiographic exposure, by increasing the concentration of tritiated thymidine and by increasing the incubation time.en
dc.language.isoenen
dc.subject.meshAutoradiography-
dc.subject.meshDNA-
dc.subject.meshDNA Repair-
dc.subject.meshHumans-
dc.subject.meshImage Processing, Computer-Assisted-
dc.subject.meshSkin-
dc.subject.meshThymidine-
dc.subject.meshTritium-
dc.subject.meshUltraviolet Rays-
dc.titleThe quantitation and kinetics of unscheduled (repair) DNA synthesis in ultraviolet-irradiated human skin by automated image analysis.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.en
dc.identifier.journalThe British Journal of Dermatologyen
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