Priming in vivo and quantification in vitro of class I MHC-restricted cytotoxic T cells to human papilloma virus type 11 early proteins (E6 and E7) using immunostimulating complexes (ISCOMs).

2.50
Hdl Handle:
http://hdl.handle.net/10541/95623
Title:
Priming in vivo and quantification in vitro of class I MHC-restricted cytotoxic T cells to human papilloma virus type 11 early proteins (E6 and E7) using immunostimulating complexes (ISCOMs).
Authors:
Tarpey, I; Stacey, S N; McIndoe, A; Davies, D H
Abstract:
Immunostimulating complexes (ISCOMs) efficiently deliver soluble antigen into both the cytosolic (endogenous) and endosomal (exogenous) pathways of antigen processing. Cytosolic delivery to antigen-presenting cells (APCs) may therefore be useful for the stimulation and assay of class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) in vitro. In this study, mice were immunized with ISCOMs containing fusion proteins of the E6 or E7 early proteins of human papilloma virus type 11 (HPV 11) to elicit CTL. These CTL were then restimulated in vitro using APCs pulsed with the same ISCOMs, prior to cytotoxicity assay using syngeneic target cells infected with recombinant vaccinia viruses. In this way, antigen-specific, MHC-restricted lysis by CD8+ cells was detected. However, this was dependent on the use of low density splenocytes as APCs for restimulation in vitro. Limiting dilution analyses showed a direct correlation between the CTL responder frequency and the number of times the animals were immunized in vivo. We conclude that in lieu of infectious virus, the use of ISCOMs to mediate antigen delivery to APCs in vitro can be used to quantitate CTL activity. This may have applications in monitoring vaccine efficacy, particularly to viruses such as HPV, which cannot be presently obtained as infectious virus in sufficient quantity for CTL propagation and assay.
Affiliation:
Division of Life Sciences, King's College, London, UK.
Citation:
Priming in vivo and quantification in vitro of class I MHC-restricted cytotoxic T cells to human papilloma virus type 11 early proteins (E6 and E7) using immunostimulating complexes (ISCOMs). 1996, 14 (3):230-6 Vaccine
Journal:
Vaccine
Issue Date:
Feb-1996
URI:
http://hdl.handle.net/10541/95623
DOI:
10.1016/0264-410X(95)00179-5
PubMed ID:
8920705
Type:
Article
Language:
en
ISSN:
0264-410X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorTarpey, Ien
dc.contributor.authorStacey, S Nen
dc.contributor.authorMcIndoe, Aen
dc.contributor.authorDavies, D Hen
dc.date.accessioned2010-04-06T09:46:11Z-
dc.date.available2010-04-06T09:46:11Z-
dc.date.issued1996-02-
dc.identifier.citationPriming in vivo and quantification in vitro of class I MHC-restricted cytotoxic T cells to human papilloma virus type 11 early proteins (E6 and E7) using immunostimulating complexes (ISCOMs). 1996, 14 (3):230-6 Vaccineen
dc.identifier.issn0264-410X-
dc.identifier.pmid8920705-
dc.identifier.doi10.1016/0264-410X(95)00179-5-
dc.identifier.urihttp://hdl.handle.net/10541/95623-
dc.description.abstractImmunostimulating complexes (ISCOMs) efficiently deliver soluble antigen into both the cytosolic (endogenous) and endosomal (exogenous) pathways of antigen processing. Cytosolic delivery to antigen-presenting cells (APCs) may therefore be useful for the stimulation and assay of class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) in vitro. In this study, mice were immunized with ISCOMs containing fusion proteins of the E6 or E7 early proteins of human papilloma virus type 11 (HPV 11) to elicit CTL. These CTL were then restimulated in vitro using APCs pulsed with the same ISCOMs, prior to cytotoxicity assay using syngeneic target cells infected with recombinant vaccinia viruses. In this way, antigen-specific, MHC-restricted lysis by CD8+ cells was detected. However, this was dependent on the use of low density splenocytes as APCs for restimulation in vitro. Limiting dilution analyses showed a direct correlation between the CTL responder frequency and the number of times the animals were immunized in vivo. We conclude that in lieu of infectious virus, the use of ISCOMs to mediate antigen delivery to APCs in vitro can be used to quantitate CTL activity. This may have applications in monitoring vaccine efficacy, particularly to viruses such as HPV, which cannot be presently obtained as infectious virus in sufficient quantity for CTL propagation and assay.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAnimals-
dc.subject.meshH-2 Antigens-
dc.subject.meshISCOMs-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshMice, Inbred DBA-
dc.subject.meshOncogene Proteins, Viral-
dc.subject.meshPapillomaviridae-
dc.subject.meshSpleen-
dc.subject.meshT-Lymphocytes, Cytotoxic-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshVaccines, Synthetic-
dc.subject.meshViral Vaccines-
dc.titlePriming in vivo and quantification in vitro of class I MHC-restricted cytotoxic T cells to human papilloma virus type 11 early proteins (E6 and E7) using immunostimulating complexes (ISCOMs).en
dc.typeArticleen
dc.contributor.departmentDivision of Life Sciences, King's College, London, UK.en
dc.identifier.journalVaccineen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.