The nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95610
Title:
The nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy.
Authors:
Goldberg, Martin W; Allen, Terence D
Abstract:
Three dimensional surface imaging was used to examine structural details of the nuclear pore complex. Subsurface structures were uncovered by detergent extraction, proteolysis, mechanical fracturing and combinations of these. Features observed in this way were mostly consistent with previous three-dimensional structures with some novel observations. In addition to cytoplasmic and basket filaments attached to each coaxial ring, we have observed radiating filaments within the central channel. New details of basket organization are presented, showing that basket filaments branch and are woven together to form the basket ring. The "central transporter" is shown to be a regular, consistent structure revealed after removal of overlying internal filaments. The lumenal ring is visualised and we present evidence that the lamina may be attached to the spoke ring complex. Finally we show that there is evidence for a novel structure, the "star ring", sandwiched between the cytoplasmic ring and the membrane.
Affiliation:
CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, UK.
Citation:
The nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy. 1996, 257 (4):848-65 J. Mol. Biol.
Journal:
Journal of Molecular Biology
Issue Date:
12-Apr-1996
URI:
http://hdl.handle.net/10541/95610
DOI:
10.1006/jmbi.1996.0206
PubMed ID:
8636986
Type:
Article
Language:
en
ISSN:
0022-2836
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGoldberg, Martin Wen
dc.contributor.authorAllen, Terence Den
dc.date.accessioned2010-04-06T09:50:43Z-
dc.date.available2010-04-06T09:50:43Z-
dc.date.issued1996-04-12-
dc.identifier.citationThe nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy. 1996, 257 (4):848-65 J. Mol. Biol.en
dc.identifier.issn0022-2836-
dc.identifier.pmid8636986-
dc.identifier.doi10.1006/jmbi.1996.0206-
dc.identifier.urihttp://hdl.handle.net/10541/95610-
dc.description.abstractThree dimensional surface imaging was used to examine structural details of the nuclear pore complex. Subsurface structures were uncovered by detergent extraction, proteolysis, mechanical fracturing and combinations of these. Features observed in this way were mostly consistent with previous three-dimensional structures with some novel observations. In addition to cytoplasmic and basket filaments attached to each coaxial ring, we have observed radiating filaments within the central channel. New details of basket organization are presented, showing that basket filaments branch and are woven together to form the basket ring. The "central transporter" is shown to be a regular, consistent structure revealed after removal of overlying internal filaments. The lumenal ring is visualised and we present evidence that the lamina may be attached to the spoke ring complex. Finally we show that there is evidence for a novel structure, the "star ring", sandwiched between the cytoplasmic ring and the membrane.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Polarity-
dc.subject.meshFemale-
dc.subject.meshHistocytological Preparation Techniques-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshModels, Structural-
dc.subject.meshNuclear Envelope-
dc.subject.meshNuclear Matrix-
dc.subject.meshOctoxynol-
dc.subject.meshOocytes-
dc.subject.meshTrypsin-
dc.subject.meshXenopus laevis-
dc.titleThe nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, UK.en
dc.identifier.journalJournal of Molecular Biologyen
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