The relative spatial distribution of in vitro-CFCs in the bone marrow, responding to specific growth factors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95606
Title:
The relative spatial distribution of in vitro-CFCs in the bone marrow, responding to specific growth factors.
Authors:
Cui, Y F; Lord, Brian I; Woolford, Lorna B; Testa, Nydia G
Abstract:
Haemopoietic progenitor cells are stimulated by a range of growth factors which promote colony growth in culture. The progenitors are a part of an age-structured developmental hierarchy in the tissue. The growth factors, although overlapping in their effects, stimulate cells preferentially at different stages in this programme. Femoral bone marrow was fractionated into axial (close to the central venous sinus) and marginal (close to the bone surface) cells. Progenitors which responded to IL-3, GM-CSF, G-CSF, M-CSF and SCF were then assayed in soft agar cultures. Consequent plots of their spatial distributions showed that the more primitive cells in vitro (responding to IL-3) were concentrated close to the bone surface. The peak concentrations of cells responding primarily to growth factors with progressively more affinity to more mature progenitor cells correspondingly appeared progressively further from the bone surface and closer to the point of release at the central venous sinus. This suggests that the developmental/maturational process in haemopoiesis is accompanied by a progressive movement of cells from the bone surface towards the central axial regions of the bone cavities. The most primitive cells are however exposed, close to the centre of the cavity, by a combination of SCF and G-CSF (or by a 50-fold increase in G-CSF concentration alone). These results corroborate earlier data which indicate a developmental movement of cells from the centre of the marrow tissue towards the bone surface and back again, sequentially encountering a series of growth factors which promote their differentiation into mature cells, for release at the central venous sinus.
Affiliation:
CRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
The relative spatial distribution of in vitro-CFCs in the bone marrow, responding to specific growth factors. 1996, 29 (5):243-57 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
May-1996
URI:
http://hdl.handle.net/10541/95606
DOI:
10.1046/j.1365-2184.1996.00999.x
PubMed ID:
8782487
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorCui, Y Fen
dc.contributor.authorLord, Brian Ien
dc.contributor.authorWoolford, Lorna Ben
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-04-06T09:37:42Z-
dc.date.available2010-04-06T09:37:42Z-
dc.date.issued1996-05-
dc.identifier.citationThe relative spatial distribution of in vitro-CFCs in the bone marrow, responding to specific growth factors. 1996, 29 (5):243-57 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid8782487-
dc.identifier.doi10.1046/j.1365-2184.1996.00999.x-
dc.identifier.urihttp://hdl.handle.net/10541/95606-
dc.description.abstractHaemopoietic progenitor cells are stimulated by a range of growth factors which promote colony growth in culture. The progenitors are a part of an age-structured developmental hierarchy in the tissue. The growth factors, although overlapping in their effects, stimulate cells preferentially at different stages in this programme. Femoral bone marrow was fractionated into axial (close to the central venous sinus) and marginal (close to the bone surface) cells. Progenitors which responded to IL-3, GM-CSF, G-CSF, M-CSF and SCF were then assayed in soft agar cultures. Consequent plots of their spatial distributions showed that the more primitive cells in vitro (responding to IL-3) were concentrated close to the bone surface. The peak concentrations of cells responding primarily to growth factors with progressively more affinity to more mature progenitor cells correspondingly appeared progressively further from the bone surface and closer to the point of release at the central venous sinus. This suggests that the developmental/maturational process in haemopoiesis is accompanied by a progressive movement of cells from the bone surface towards the central axial regions of the bone cavities. The most primitive cells are however exposed, close to the centre of the cavity, by a combination of SCF and G-CSF (or by a 50-fold increase in G-CSF concentration alone). These results corroborate earlier data which indicate a developmental movement of cells from the centre of the marrow tissue towards the bone surface and back again, sequentially encountering a series of growth factors which promote their differentiation into mature cells, for release at the central venous sinus.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Movement-
dc.subject.meshColony-Stimulating Factors-
dc.subject.meshFemur-
dc.subject.meshGranulocyte Colony-Stimulating Factor-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInterleukin-3-
dc.subject.meshMacrophage Colony-Stimulating Factor-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshMice, Inbred DBA-
dc.subject.meshStem Cell Factor-
dc.titleThe relative spatial distribution of in vitro-CFCs in the bone marrow, responding to specific growth factors.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalCell Proliferationen

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