2.50
Hdl Handle:
http://hdl.handle.net/10541/95603
Title:
Transferrin-mediated uptake of radionuclides by the testis.
Authors:
Hoyes, Katherine P; Morris, Ian D; Hendry, Jolyon H; Sharma, Harbans L
Abstract:
In an attempt to explain the deleterious effects of gonadal radionuclide localization, we examined the role of transferrin in testicular radionuclide uptake. METHODS: In vivo testicular uptake and retention of the transferrin binding radionuclides 114mIn-citrate and 59Fe-citrate were compared with that of the nontransferrin binding isotopes 137Cs-citrate and Na125I for 63 days postinjection. Isotope uptake mechanisms were investigated in vitro using isolated seminiferous tubules and Sertoli cell monolayers grown in bicameral culture chambers. RESULTS: Indium-114m, 59Fe and 137Cs were localized in the testis by 24 hr postinjection, but accumulation of 125I was minimal. Although testicular 114mIn remained constant, 59Fe declined slowly over the following 63 days and 137Cs fell very rapidly. When 114mIn- or 59Fe-loaded testes were fractionated, and markedly more 114mIn was associated with the seminiferous tubules than 59Fe, suggesting that 114mIn may be retained. In vitro uptake of 59Fe, 67Ga and 114mIn by isolated seminiferous tubules was inhibited by transferrin, but uptake of 137Cs and 125I was unaffected. Iron-59, 67Ga and 114mIn were retained by isolated tubules in contrast to 137Cs and 125I. Whereas 137Cs, 59Fe and 114mIn all crossed Sertoli cell monolayers, the rate of transcellular transport of 137Cs was faster than that of 59Fe or 114mIn, suggesting differences in the intracellular processing of transferrin binding and nontransferrin binding radionuclides. CONCLUSION: These data suggest that some radionuclides may access the seminiferous epithelium through receptor-mediated endocytosis of transferrin. Such radionuclide localization could lead to continuous irradiation of the testes, resulting in mutagenic damage to spermatogenic cells.
Affiliation:
Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, United Kingdom.
Citation:
Transferrin-mediated uptake of radionuclides by the testis. 1996, 37 (2):336-40 J. Nucl. Med.
Journal:
Journal of Nuclear Medicine
Issue Date:
Feb-1996
URI:
http://hdl.handle.net/10541/95603
PubMed ID:
8667073
Type:
Article
Language:
en
ISSN:
0161-5505
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHoyes, Katherine Pen
dc.contributor.authorMorris, Ian Den
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorSharma, Harbans Len
dc.date.accessioned2010-04-06T09:27:05Z-
dc.date.available2010-04-06T09:27:05Z-
dc.date.issued1996-02-
dc.identifier.citationTransferrin-mediated uptake of radionuclides by the testis. 1996, 37 (2):336-40 J. Nucl. Med.en
dc.identifier.issn0161-5505-
dc.identifier.pmid8667073-
dc.identifier.urihttp://hdl.handle.net/10541/95603-
dc.description.abstractIn an attempt to explain the deleterious effects of gonadal radionuclide localization, we examined the role of transferrin in testicular radionuclide uptake. METHODS: In vivo testicular uptake and retention of the transferrin binding radionuclides 114mIn-citrate and 59Fe-citrate were compared with that of the nontransferrin binding isotopes 137Cs-citrate and Na125I for 63 days postinjection. Isotope uptake mechanisms were investigated in vitro using isolated seminiferous tubules and Sertoli cell monolayers grown in bicameral culture chambers. RESULTS: Indium-114m, 59Fe and 137Cs were localized in the testis by 24 hr postinjection, but accumulation of 125I was minimal. Although testicular 114mIn remained constant, 59Fe declined slowly over the following 63 days and 137Cs fell very rapidly. When 114mIn- or 59Fe-loaded testes were fractionated, and markedly more 114mIn was associated with the seminiferous tubules than 59Fe, suggesting that 114mIn may be retained. In vitro uptake of 59Fe, 67Ga and 114mIn by isolated seminiferous tubules was inhibited by transferrin, but uptake of 137Cs and 125I was unaffected. Iron-59, 67Ga and 114mIn were retained by isolated tubules in contrast to 137Cs and 125I. Whereas 137Cs, 59Fe and 114mIn all crossed Sertoli cell monolayers, the rate of transcellular transport of 137Cs was faster than that of 59Fe or 114mIn, suggesting differences in the intracellular processing of transferrin binding and nontransferrin binding radionuclides. CONCLUSION: These data suggest that some radionuclides may access the seminiferous epithelium through receptor-mediated endocytosis of transferrin. Such radionuclide localization could lead to continuous irradiation of the testes, resulting in mutagenic damage to spermatogenic cells.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBlood-Testis Barrier-
dc.subject.meshCells, Cultured-
dc.subject.meshCesium Radioisotopes-
dc.subject.meshEndocytosis-
dc.subject.meshIndium Radioisotopes-
dc.subject.meshIodine Radioisotopes-
dc.subject.meshIron-
dc.subject.meshIron Radioisotopes-
dc.subject.meshMale-
dc.subject.meshRats-
dc.subject.meshRats, Sprague-Dawley-
dc.subject.meshSeminiferous Tubules-
dc.subject.meshSertoli Cells-
dc.subject.meshTestis-
dc.subject.meshTime Factors-
dc.subject.meshTissue Distribution-
dc.subject.meshTransferrin-
dc.titleTransferrin-mediated uptake of radionuclides by the testis.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, United Kingdom.en
dc.identifier.journalJournal of Nuclear Medicineen

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