Multilineage gene expression precedes commitment in the hemopoietic system.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95475
Title:
Multilineage gene expression precedes commitment in the hemopoietic system.
Authors:
Hu, M; Krause, D; Greaves, M; Sharkis, S; Dexter, T Michael; Heyworth, Clare M; Enver, T
Abstract:
We have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (beta-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. Furthermore, the multipotential state is characterized by the coexpression of several lineage-affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a "promiscuous" phase of multilineage locus activation.
Affiliation:
The Leukaemia Research Fund Centre at the Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Citation:
Multilineage gene expression precedes commitment in the hemopoietic system. 1997, 11 (6):774-85 Genes Dev.
Journal:
Genes & Development
Issue Date:
15-Mar-1997
URI:
http://hdl.handle.net/10541/95475
DOI:
10.1101/gad.11.6.774
PubMed ID:
9087431
Type:
Article
Language:
en
ISSN:
0890-9369
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHu, Men
dc.contributor.authorKrause, Den
dc.contributor.authorGreaves, Men
dc.contributor.authorSharkis, Sen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorEnver, Ten
dc.date.accessioned2010-04-01T13:29:58Z-
dc.date.available2010-04-01T13:29:58Z-
dc.date.issued1997-03-15-
dc.identifier.citationMultilineage gene expression precedes commitment in the hemopoietic system. 1997, 11 (6):774-85 Genes Dev.en
dc.identifier.issn0890-9369-
dc.identifier.pmid9087431-
dc.identifier.doi10.1101/gad.11.6.774-
dc.identifier.urihttp://hdl.handle.net/10541/95475-
dc.description.abstractWe have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (beta-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. Furthermore, the multipotential state is characterized by the coexpression of several lineage-affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a "promiscuous" phase of multilineage locus activation.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshCell Line-
dc.subject.meshErythropoiesis-
dc.subject.meshGene Expression-
dc.subject.meshGlobins-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshMice-
dc.subject.meshModels, Biological-
dc.subject.meshPeroxidase-
dc.subject.meshReceptors, Cytokine-
dc.subject.meshReceptors, Growth Factor-
dc.titleMultilineage gene expression precedes commitment in the hemopoietic system.en
dc.typeArticleen
dc.contributor.departmentThe Leukaemia Research Fund Centre at the Institute of Cancer Research, Chester Beatty Laboratories, London, UK.en
dc.identifier.journalGenes & Developmenten

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