High-dose granulocyte-colony stimulating factor (G-CSF) in vitro induces the growth of high proliferative potential colony forming cells (HPP-CFC) in patients undergoing blood stem cell mobilization.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95465
Title:
High-dose granulocyte-colony stimulating factor (G-CSF) in vitro induces the growth of high proliferative potential colony forming cells (HPP-CFC) in patients undergoing blood stem cell mobilization.
Authors:
Perez-Oteyza, J; Ramos, P; Testa, Nydia G; Heinrichs, B; Lopez-Jimenez, J; Garcia-Laraña, J; Odriozola, J
Abstract:
We evaluated the role of high-dose granulocyte colony stimulating factor (G-CSF) in vitro, in inducing the generation of high-proliferative potential colony forming cells (HPP-CFC), from either mononuclear cells or purified CD34+ cells. Both normal controls and patients undergoing peripheral blood stem cell (PBSC) mobilization and transplantation were studied. In serum-driven agar cultures, G-CSF stimulated the proliferation of HPP-CFC in a dose dependent manner (r = 0.92). The number of HPP-CFC was four-fold greater in mobilized patients than in normal controls. Purified CD34+ cells yielded 11-fold more colonies than mononuclear cells. HPP-CFC from mobilized patients showed replating capacity, giving rise to secondary colonies of more mature appearance. In serum-free cultures, the effect of G-CSF appeared to be mediated by synergistic interaction with stem cell factor. Our results suggest that G-CSF stimulates primitive hematopoietic cells that are detectable in increased amounts in patients receiving mobilization therapy. Therefore, determination of G-CSF induced HPP-CFC could be a useful tool in the evaluation of mobilization strategies.
Affiliation:
Department of Hematology, Hospital Ramon y Cajal, Madrid, Spain.
Citation:
High-dose granulocyte-colony stimulating factor (G-CSF) in vitro induces the growth of high proliferative potential colony forming cells (HPP-CFC) in patients undergoing blood stem cell mobilization. 1997, 25 (6):516-20 Exp. Hematol.
Journal:
Experimental Hematology
Issue Date:
Jun-1997
URI:
http://hdl.handle.net/10541/95465
PubMed ID:
9197330
Type:
Article
Language:
en
ISSN:
0301-472X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPerez-Oteyza, Jen
dc.contributor.authorRamos, Pen
dc.contributor.authorTesta, Nydia Gen
dc.contributor.authorHeinrichs, Ben
dc.contributor.authorLopez-Jimenez, Jen
dc.contributor.authorGarcia-Laraña, Jen
dc.contributor.authorOdriozola, Jen
dc.date.accessioned2010-04-01T11:18:53Z-
dc.date.available2010-04-01T11:18:53Z-
dc.date.issued1997-06-
dc.identifier.citationHigh-dose granulocyte-colony stimulating factor (G-CSF) in vitro induces the growth of high proliferative potential colony forming cells (HPP-CFC) in patients undergoing blood stem cell mobilization. 1997, 25 (6):516-20 Exp. Hematol.en
dc.identifier.issn0301-472X-
dc.identifier.pmid9197330-
dc.identifier.urihttp://hdl.handle.net/10541/95465-
dc.description.abstractWe evaluated the role of high-dose granulocyte colony stimulating factor (G-CSF) in vitro, in inducing the generation of high-proliferative potential colony forming cells (HPP-CFC), from either mononuclear cells or purified CD34+ cells. Both normal controls and patients undergoing peripheral blood stem cell (PBSC) mobilization and transplantation were studied. In serum-driven agar cultures, G-CSF stimulated the proliferation of HPP-CFC in a dose dependent manner (r = 0.92). The number of HPP-CFC was four-fold greater in mobilized patients than in normal controls. Purified CD34+ cells yielded 11-fold more colonies than mononuclear cells. HPP-CFC from mobilized patients showed replating capacity, giving rise to secondary colonies of more mature appearance. In serum-free cultures, the effect of G-CSF appeared to be mediated by synergistic interaction with stem cell factor. Our results suggest that G-CSF stimulates primitive hematopoietic cells that are detectable in increased amounts in patients receiving mobilization therapy. Therefore, determination of G-CSF induced HPP-CFC could be a useful tool in the evaluation of mobilization strategies.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAntigens, CD34-
dc.subject.meshBlood-
dc.subject.meshCell Separation-
dc.subject.meshCells, Cultured-
dc.subject.meshCulture Media-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshGranulocyte Colony-Stimulating Factor-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.titleHigh-dose granulocyte-colony stimulating factor (G-CSF) in vitro induces the growth of high proliferative potential colony forming cells (HPP-CFC) in patients undergoing blood stem cell mobilization.en
dc.typeArticleen
dc.contributor.departmentDepartment of Hematology, Hospital Ramon y Cajal, Madrid, Spain.en
dc.identifier.journalExperimental Hematologyen
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