Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95443
Title:
Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures.
Authors:
Rutherford, Sandra A; Goldberg, Martin W; Allen, Terence D
Abstract:
The three-dimensional localization of nucleoplasmin and wheat germ agglutinin (WGA) at the nuclear envelope of Xenopus oocytes is demonstrated by microinjecting protein coated gold colloids and examining their distribution using both stereo transmission electron microscopy and field emission in-lens scanning electron microscopy. Localization of many WGA gold particles and nucleoplasmin gold particles at the same nuclear pore complex (NPC) following coinjection is demonstrated. Binding of the WGA gold in the central region of the NPCs appears to form a barrier, preventing the import of nucleoplasmin gold, and includes central localization along radial "tracks" which correspond to the internal filaments connecting the cytoplasmic ring and the central region of the NPC. We suggest that these filaments may in some way be involved in opening and closing of the central channel of the NPC for transport. Transport of nucleoplasmin through the central region of the NPCs appears to be in "single file" regardless of the size of the colloidal gold, and distribution into the nucleoplasm appears to be through the basket rings with no association of the nucleoplasmin gold with the basket filaments being observed.
Affiliation:
CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom.
Citation:
Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures. 1997, 232 (1):146-60 Exp. Cell Res.
Journal:
Experimental Cell Research
Issue Date:
10-Apr-1997
URI:
http://hdl.handle.net/10541/95443
DOI:
10.1006/excr.1997.3487
PubMed ID:
9141631
Type:
Article
Language:
en
ISSN:
0014-4827
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorRutherford, Sandra Aen
dc.contributor.authorGoldberg, Martin Wen
dc.contributor.authorAllen, Terence Den
dc.date.accessioned2010-04-01T11:26:20Z-
dc.date.available2010-04-01T11:26:20Z-
dc.date.issued1997-04-10-
dc.identifier.citationThree-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures. 1997, 232 (1):146-60 Exp. Cell Res.en
dc.identifier.issn0014-4827-
dc.identifier.pmid9141631-
dc.identifier.doi10.1006/excr.1997.3487-
dc.identifier.urihttp://hdl.handle.net/10541/95443-
dc.description.abstractThe three-dimensional localization of nucleoplasmin and wheat germ agglutinin (WGA) at the nuclear envelope of Xenopus oocytes is demonstrated by microinjecting protein coated gold colloids and examining their distribution using both stereo transmission electron microscopy and field emission in-lens scanning electron microscopy. Localization of many WGA gold particles and nucleoplasmin gold particles at the same nuclear pore complex (NPC) following coinjection is demonstrated. Binding of the WGA gold in the central region of the NPCs appears to form a barrier, preventing the import of nucleoplasmin gold, and includes central localization along radial "tracks" which correspond to the internal filaments connecting the cytoplasmic ring and the central region of the NPC. We suggest that these filaments may in some way be involved in opening and closing of the central channel of the NPC for transport. Transport of nucleoplasmin through the central region of the NPCs appears to be in "single file" regardless of the size of the colloidal gold, and distribution into the nucleoplasm appears to be through the basket rings with no association of the nucleoplasmin gold with the basket filaments being observed.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBiological Transport-
dc.subject.meshFemale-
dc.subject.meshGold Colloid-
dc.subject.meshMicroinjections-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMicroscopy, Electron, Scanning Transmission-
dc.subject.meshModels, Biological-
dc.subject.meshNuclear Envelope-
dc.subject.meshNuclear Proteins-
dc.subject.meshNucleoplasmins-
dc.subject.meshOocytes-
dc.subject.meshPhosphoproteins-
dc.subject.meshWheat Germ Agglutinins-
dc.subject.meshXenopus-
dc.titleThree-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom.en
dc.identifier.journalExperimental Cell Researchen

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