Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures.
Affiliation
CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom.Issue Date
1997-04-10
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The three-dimensional localization of nucleoplasmin and wheat germ agglutinin (WGA) at the nuclear envelope of Xenopus oocytes is demonstrated by microinjecting protein coated gold colloids and examining their distribution using both stereo transmission electron microscopy and field emission in-lens scanning electron microscopy. Localization of many WGA gold particles and nucleoplasmin gold particles at the same nuclear pore complex (NPC) following coinjection is demonstrated. Binding of the WGA gold in the central region of the NPCs appears to form a barrier, preventing the import of nucleoplasmin gold, and includes central localization along radial "tracks" which correspond to the internal filaments connecting the cytoplasmic ring and the central region of the NPC. We suggest that these filaments may in some way be involved in opening and closing of the central channel of the NPC for transport. Transport of nucleoplasmin through the central region of the NPCs appears to be in "single file" regardless of the size of the colloidal gold, and distribution into the nucleoplasm appears to be through the basket rings with no association of the nucleoplasmin gold with the basket filaments being observed.Citation
Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures. 1997, 232 (1):146-60 Exp. Cell Res.Journal
Experimental Cell ResearchDOI
10.1006/excr.1997.3487PubMed ID
9141631Type
ArticleLanguage
enISSN
0014-4827ae974a485f413a2113503eed53cd6c53
10.1006/excr.1997.3487
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