Macromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95247
Title:
Macromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy.
Authors:
Allen, Terence D; Bennion, G R; Rutherford, S A; Reipert, Siegfried; Ramalho, A; Kiseleva, Elena; Goldberg, Martin W
Abstract:
Scanning electron microscopy (SEM) has produced a wealth of novel images that have significantly complemented our perception of biological structure and function, derived initially from transmission electron microscopy (TEM) information. SEM is a surface imaging technology, and its impact at the subcellular level has been restricted by reduced resolution in comparison with TEM. Recently, SEM resolution has been considerably improved by the advent of high-brightness sources used in field-emission instruments (FEISEM) which have produced resolution of around 1 nm, virtually equivalent to TEM "working resolution." Here we review our findings in the use of FEISEM in the imaging of nuclear envelopes and their associated structures, such as nuclear pore complexes, and the relationships of structure and function. FEISEM allows the structurally orientated cell biologist to visualise, directly and in three dimensions, subcellular structure and its modulation with a view to understanding its functional significance.
Affiliation:
CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
Citation:
Macromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy. 1997, 19 (6):403-10 Scanning
Journal:
Scanning
Issue Date:
Sep-1997
URI:
http://hdl.handle.net/10541/95247
DOI:
10.1002/sca.4950190603
PubMed ID:
9304867
Type:
Article
Language:
en
ISSN:
0161-0457
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorAllen, Terence Den
dc.contributor.authorBennion, G Ren
dc.contributor.authorRutherford, S Aen
dc.contributor.authorReipert, Siegfrieden
dc.contributor.authorRamalho, Aen
dc.contributor.authorKiseleva, Elenaen
dc.contributor.authorGoldberg, Martin Wen
dc.date.accessioned2010-03-30T13:27:34Z-
dc.date.available2010-03-30T13:27:34Z-
dc.date.issued1997-09-
dc.identifier.citationMacromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy. 1997, 19 (6):403-10 Scanningen
dc.identifier.issn0161-0457-
dc.identifier.pmid9304867-
dc.identifier.doi10.1002/sca.4950190603-
dc.identifier.urihttp://hdl.handle.net/10541/95247-
dc.description.abstractScanning electron microscopy (SEM) has produced a wealth of novel images that have significantly complemented our perception of biological structure and function, derived initially from transmission electron microscopy (TEM) information. SEM is a surface imaging technology, and its impact at the subcellular level has been restricted by reduced resolution in comparison with TEM. Recently, SEM resolution has been considerably improved by the advent of high-brightness sources used in field-emission instruments (FEISEM) which have produced resolution of around 1 nm, virtually equivalent to TEM "working resolution." Here we review our findings in the use of FEISEM in the imaging of nuclear envelopes and their associated structures, such as nuclear pore complexes, and the relationships of structure and function. FEISEM allows the structurally orientated cell biologist to visualise, directly and in three dimensions, subcellular structure and its modulation with a view to understanding its functional significance.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Nucleus-
dc.subject.meshCells, Cultured-
dc.subject.meshChironomidae-
dc.subject.meshCytoplasm-
dc.subject.meshDetergents-
dc.subject.meshEndopeptidases-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshNuclear Envelope-
dc.subject.meshOocytes-
dc.subject.meshResins, Plant-
dc.subject.meshSalivary Glands-
dc.subject.meshWater-
dc.subject.meshXenopus laevis-
dc.titleMacromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.en
dc.identifier.journalScanningen

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