2.50
Hdl Handle:
http://hdl.handle.net/10541/95227
Title:
Nuclear pore complex structure in birds.
Authors:
Goldberg, Martin W; Solovei, I; Allen, Terence D
Abstract:
The nuclear envelope consists of two parallel membranes enclosing an aqueous lumen. In places there are pores in both membranes at which the two membranes are joined. Within these pores reside the nuclear pore complexes. The current structural models of the nuclear pore complex have been derived from a number of studies using different electron microscopical techniques. Recently, using surface imaging techniques such as field emission in-lens scanning electron microscopy, novel structures have been identified, particularly at the periphery of the structure, most notably the nucleoplasmic basket. One limitation of the current models is that they are based almost entirely on nuclear envelopes isolated from amphibian oocytes and a pressing question is whether this structure is the same in other organisms and tissues. Here we have studied the structure of nuclear envelopes isolated from bird oocytes. We show that the overall structure is remarkably conserved. In particular, recently discovered peripheral structures appear very similar. We see variations in basket conformation but believe that this is related to the functional states of individual pore complexes.
Affiliation:
Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom.
Citation:
Nuclear pore complex structure in birds. 1997, 119 (3):284-94 J. Struct. Biol.
Journal:
Journal of Structural Biology
Issue Date:
Aug-1997
URI:
http://hdl.handle.net/10541/95227
DOI:
10.1006/jsbi.1997.3877
PubMed ID:
9255965
Type:
Article
Language:
en
ISSN:
1047-8477
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGoldberg, Martin Wen
dc.contributor.authorSolovei, Ien
dc.contributor.authorAllen, Terence Den
dc.date.accessioned2010-03-30T10:56:52Z-
dc.date.available2010-03-30T10:56:52Z-
dc.date.issued1997-08-
dc.identifier.citationNuclear pore complex structure in birds. 1997, 119 (3):284-94 J. Struct. Biol.en
dc.identifier.issn1047-8477-
dc.identifier.pmid9255965-
dc.identifier.doi10.1006/jsbi.1997.3877-
dc.identifier.urihttp://hdl.handle.net/10541/95227-
dc.description.abstractThe nuclear envelope consists of two parallel membranes enclosing an aqueous lumen. In places there are pores in both membranes at which the two membranes are joined. Within these pores reside the nuclear pore complexes. The current structural models of the nuclear pore complex have been derived from a number of studies using different electron microscopical techniques. Recently, using surface imaging techniques such as field emission in-lens scanning electron microscopy, novel structures have been identified, particularly at the periphery of the structure, most notably the nucleoplasmic basket. One limitation of the current models is that they are based almost entirely on nuclear envelopes isolated from amphibian oocytes and a pressing question is whether this structure is the same in other organisms and tissues. Here we have studied the structure of nuclear envelopes isolated from bird oocytes. We show that the overall structure is remarkably conserved. In particular, recently discovered peripheral structures appear very similar. We see variations in basket conformation but believe that this is related to the functional states of individual pore complexes.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshChickens-
dc.subject.meshFemale-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshNuclear Envelope-
dc.subject.meshOocytes-
dc.subject.meshSpecies Specificity-
dc.subject.meshXenopus laevis-
dc.titleNuclear pore complex structure in birds.en
dc.typeArticleen
dc.contributor.departmentDepartment of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom.en
dc.identifier.journalJournal of Structural Biologyen
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