3H-thymidine labelling index (TLI) as a marker of tumour growth heterogeneity: evaluation in human solid carcinomas.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95186
Title:
3H-thymidine labelling index (TLI) as a marker of tumour growth heterogeneity: evaluation in human solid carcinomas.
Authors:
Becciolini, A; Balzi, M; Barbarisi, M; Faraoni, P; Biggeri, A; Potten, Christopher S
Abstract:
Many studies deal with the analysis of cell kinetic, cytogenetic, biochemical and molecular cell biology parameters to identify prognostic factors relating to tumour growth but all methods use only a small part of the total tumour mass. This study is devoted to the analysis of the heterogeneity of the growth of human solid tumours assaying proliferative activity by means of 3H-thymidine labelling index (TLI) in a fixed number of samples collected in different areas of the lesion (larynx and colon cancers), or in different lesions of the same subject (breast and bladder cancers). Each sample (at the macroscopic level) was divided into small fragments (at the microscopic level) and proliferative activity was determined. The analysis of variance for hierarchical designs demonstrated that in all cases a high component of the variance is attributable to the subjects and to the fragments whereas the variance attributable to the different areas is very low. The heterogeneity of proliferative activity displays a higher focal variability among the fragments (microscopic level) compared with that among areas (macroscopic level) within subjects, provided an adequate number of fragments and cells are counted. In multiple synchronous carcinoma of the bladder the wide variability of proliferation among the single lesions demonstrated that it is necessary to analyse all the tumours in a subject because each one is characterized by a different cell growth potential.
Affiliation:
Department of Clinical Physiopathology, University of Florence, Italy.
Citation:
3H-thymidine labelling index (TLI) as a marker of tumour growth heterogeneity: evaluation in human solid carcinomas., 30 (3-4):117-26 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
1997
URI:
http://hdl.handle.net/10541/95186
DOI:
10.1111/j.1365-2184.1997.tb00928.x
PubMed ID:
9375024
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBecciolini, Aen
dc.contributor.authorBalzi, Men
dc.contributor.authorBarbarisi, Men
dc.contributor.authorFaraoni, Pen
dc.contributor.authorBiggeri, Aen
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-03-29T15:27:30Z-
dc.date.available2010-03-29T15:27:30Z-
dc.date.issued1997-
dc.identifier.citation3H-thymidine labelling index (TLI) as a marker of tumour growth heterogeneity: evaluation in human solid carcinomas., 30 (3-4):117-26 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid9375024-
dc.identifier.doi10.1111/j.1365-2184.1997.tb00928.x-
dc.identifier.urihttp://hdl.handle.net/10541/95186-
dc.description.abstractMany studies deal with the analysis of cell kinetic, cytogenetic, biochemical and molecular cell biology parameters to identify prognostic factors relating to tumour growth but all methods use only a small part of the total tumour mass. This study is devoted to the analysis of the heterogeneity of the growth of human solid tumours assaying proliferative activity by means of 3H-thymidine labelling index (TLI) in a fixed number of samples collected in different areas of the lesion (larynx and colon cancers), or in different lesions of the same subject (breast and bladder cancers). Each sample (at the macroscopic level) was divided into small fragments (at the microscopic level) and proliferative activity was determined. The analysis of variance for hierarchical designs demonstrated that in all cases a high component of the variance is attributable to the subjects and to the fragments whereas the variance attributable to the different areas is very low. The heterogeneity of proliferative activity displays a higher focal variability among the fragments (microscopic level) compared with that among areas (macroscopic level) within subjects, provided an adequate number of fragments and cells are counted. In multiple synchronous carcinoma of the bladder the wide variability of proliferation among the single lesions demonstrated that it is necessary to analyse all the tumours in a subject because each one is characterized by a different cell growth potential.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectColonic Canceren
dc.subjectLaryngeal Canceren
dc.subjectUrinary Bladder Canceren
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshBreast Neoplasms-
dc.subject.meshCarcinoma-
dc.subject.meshCell Division-
dc.subject.meshColonic Neoplasms-
dc.subject.meshCulture Techniques-
dc.subject.meshEvaluation Studies as Topic-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshLaryngeal Neoplasms-
dc.subject.meshMiddle Aged-
dc.subject.meshMitotic Index-
dc.subject.meshThymidine-
dc.subject.meshTritium-
dc.subject.meshUrinary Bladder Neoplasms-
dc.title3H-thymidine labelling index (TLI) as a marker of tumour growth heterogeneity: evaluation in human solid carcinomas.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Physiopathology, University of Florence, Italy.en
dc.identifier.journalCell Proliferationen

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