A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95177
Title:
A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin.
Authors:
McNair, Fiona I; Marples, Brian; West, Catharine M L; Moore, James V
Abstract:
Single-cell electrophoresis (comet assay) has been used to evaluate DNA damage and repair in the human myeloid leukaemia cell line K562 after low-dose (predominantly sub-lethal) treatments of hyperthermia and photodynamic therapy (PDT). Three different photosensitizers were examined: haematoporphyrin derivative (HpD), methylene blue (MB) and meso-tetrahydroxyphenylchlorin (mTHPC). None of the drugs in the absence of light, nor in light alone, resulted in detectable DNA damage. However, a significant amount of DNA damage was detected immediately after treatment with haematoporphyrin derivative or methylene blue PDT compared with drug-only or light-only treatments; no residual level of DNA damage was evident for either drug following a 4-h post-treatment incubation at 37 degrees C. No significant DNA damage was detected after meso-tetrahydroxyphenylchlorin PDT or hyperthermia either immediately or 4 h after treatment. We conclude that the alkaline comet assay can be applied as an effective screening assay for DNA damage induced by a range of laser therapies.
Affiliation:
Laser Oncology Programme and Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin. 1997, 75 (12):1721-9 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
1997
URI:
http://hdl.handle.net/10541/95177
PubMed ID:
9192973
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMcNair, Fiona Ien
dc.contributor.authorMarples, Brianen
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorMoore, James Ven
dc.date.accessioned2010-03-29T14:08:25Z-
dc.date.available2010-03-29T14:08:25Z-
dc.date.issued1997-
dc.identifier.citationA comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin. 1997, 75 (12):1721-9 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid9192973-
dc.identifier.urihttp://hdl.handle.net/10541/95177-
dc.description.abstractSingle-cell electrophoresis (comet assay) has been used to evaluate DNA damage and repair in the human myeloid leukaemia cell line K562 after low-dose (predominantly sub-lethal) treatments of hyperthermia and photodynamic therapy (PDT). Three different photosensitizers were examined: haematoporphyrin derivative (HpD), methylene blue (MB) and meso-tetrahydroxyphenylchlorin (mTHPC). None of the drugs in the absence of light, nor in light alone, resulted in detectable DNA damage. However, a significant amount of DNA damage was detected immediately after treatment with haematoporphyrin derivative or methylene blue PDT compared with drug-only or light-only treatments; no residual level of DNA damage was evident for either drug following a 4-h post-treatment incubation at 37 degrees C. No significant DNA damage was detected after meso-tetrahydroxyphenylchlorin PDT or hyperthermia either immediately or 4 h after treatment. We conclude that the alkaline comet assay can be applied as an effective screening assay for DNA damage induced by a range of laser therapies.en
dc.language.isoenen
dc.subjectHaematoporphyrin Derivativeen
dc.subjectCultured Tumour Cellsen
dc.subjectMyeloid Leukaemiaen
dc.subject.meshDNA Damage-
dc.subject.meshDNA Repair-
dc.subject.meshElectrophoresis, Agar Gel-
dc.subject.meshHematoporphyrin Derivative-
dc.subject.meshHumans-
dc.subject.meshHyperthermia, Induced-
dc.subject.meshLeukemia, Myeloid-
dc.subject.meshMesoporphyrins-
dc.subject.meshMethylene Blue-
dc.subject.meshPhotochemotherapy-
dc.subject.meshPhotosensitizing Agents-
dc.subject.meshTumor Cells, Cultured-
dc.titleA comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin.en
dc.typeArticleen
dc.contributor.departmentLaser Oncology Programme and Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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