Increased expression of TGF-beta 1 in brain tissue after ischemic stroke in humans.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95146
Title:
Increased expression of TGF-beta 1 in brain tissue after ischemic stroke in humans.
Authors:
Krupinski, Jerzy; Kumar, Patricia; Kumar, Shant; Kaluza, J
Abstract:
BACKGROUND AND PURPOSE: Occlusion in cerebral vessels results in ischemic stroke and is followed by proliferation of microvessels, ie, angiogenesis. The process is particularly marked in the border zone of the infarct, known as the ischemic penumbra. This increase in vascularization is likely to be caused by the action of angiogenic factors, such as TGF-beta 1, which is a powerful regulator of angiogenesis. METHODS: In this study we examined 10 brain samples from patients who suffered from ischemic stroke for the expression of mRNA encoding TGF-beta 1. RESULTS: The ischemic penumbra contained the highest levels of TGF-beta 1 mRNA, whereas the normal contralateral hemispheres had the least (P < .001, Mann-Whitney U test). Unlike those from normal brain, protein extracts from infarcted tissue contained active TGF-beta 1 as a 25-kD band in Western blot analysis. Extracts from the penumbra also contained a 12.5-kD isoform of TGF-beta 1. Both penumbra and infarct contained TGF-beta 1 immunoreactive products as assessed with immunohistochemistry, whereas very weak staining was observed in the contralateral hemisphere. CONCLUSIONS: These results suggest that TGF-beta 1 is important in the pathogenesis of the angiogenic response in ischemic brain tissue and its modulation may be used for therapeutic purposes.
Affiliation:
Department of Biological Sciences, Manchester Metropolitan University, UK.
Citation:
Increased expression of TGF-beta 1 in brain tissue after ischemic stroke in humans. 1996, 27 (5):852-7 Stroke
Journal:
Stroke
Issue Date:
May-1996
URI:
http://hdl.handle.net/10541/95146
PubMed ID:
8623105
Type:
Article
Language:
en
ISSN:
0039-2499
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorKrupinski, Jerzyen
dc.contributor.authorKumar, Patriciaen
dc.contributor.authorKumar, Shanten
dc.contributor.authorKaluza, Jen
dc.date.accessioned2010-03-29T12:10:19Z-
dc.date.available2010-03-29T12:10:19Z-
dc.date.issued1996-05-
dc.identifier.citationIncreased expression of TGF-beta 1 in brain tissue after ischemic stroke in humans. 1996, 27 (5):852-7 Strokeen
dc.identifier.issn0039-2499-
dc.identifier.pmid8623105-
dc.identifier.urihttp://hdl.handle.net/10541/95146-
dc.description.abstractBACKGROUND AND PURPOSE: Occlusion in cerebral vessels results in ischemic stroke and is followed by proliferation of microvessels, ie, angiogenesis. The process is particularly marked in the border zone of the infarct, known as the ischemic penumbra. This increase in vascularization is likely to be caused by the action of angiogenic factors, such as TGF-beta 1, which is a powerful regulator of angiogenesis. METHODS: In this study we examined 10 brain samples from patients who suffered from ischemic stroke for the expression of mRNA encoding TGF-beta 1. RESULTS: The ischemic penumbra contained the highest levels of TGF-beta 1 mRNA, whereas the normal contralateral hemispheres had the least (P < .001, Mann-Whitney U test). Unlike those from normal brain, protein extracts from infarcted tissue contained active TGF-beta 1 as a 25-kD band in Western blot analysis. Extracts from the penumbra also contained a 12.5-kD isoform of TGF-beta 1. Both penumbra and infarct contained TGF-beta 1 immunoreactive products as assessed with immunohistochemistry, whereas very weak staining was observed in the contralateral hemisphere. CONCLUSIONS: These results suggest that TGF-beta 1 is important in the pathogenesis of the angiogenic response in ischemic brain tissue and its modulation may be used for therapeutic purposes.en
dc.language.isoenen
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshAstrocytes-
dc.subject.meshAutopsy-
dc.subject.meshBlotting, Western-
dc.subject.meshBrain-
dc.subject.meshBrain Ischemia-
dc.subject.meshCerebellum-
dc.subject.meshCerebral Infarction-
dc.subject.meshDNA Probes-
dc.subject.meshEndothelium, Vascular-
dc.subject.meshFemale-
dc.subject.meshFunctional Laterality-
dc.subject.meshGene Expression-
dc.subject.meshHumans-
dc.subject.meshIn Situ Hybridization-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshNeurons-
dc.subject.meshRNA, Messenger-
dc.subject.meshTransforming Growth Factor beta-
dc.titleIncreased expression of TGF-beta 1 in brain tissue after ischemic stroke in humans.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biological Sciences, Manchester Metropolitan University, UK.en
dc.identifier.journalStrokeen

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