Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/94933
Title:
Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells.
Authors:
Hampson, Ian N; Hampson, Lynne; Pinkoski, M; Cross, M; Heyworth, Clare M; Bleackley, R C; Atkinson, E; Dexter, T Michael
Abstract:
We have identified a gene that has a high level of mRNA expression in undifferentiated, multipotential hematopoietic cells (FDCP-Mix) and that downregulates both transcript and protein, as these cells are induced to differentiate into mature myeloid cells. Sequence analysis of this gene has identified it as a serine protease inhibitor EB22/3 (serpin 2A). Constitutive expression of serpin 2A in FDCP-Mix cells was associated with an increase in the clonogenic potential of the cells and with a delay in the appearance of fully mature cells in cultures undergoing granulocyte macrophage differentiation when compared with control cells. Serpin 2A was also found to be expressed in bone marrow-derived bipotent granulocyte macrophage progenitor cells (GM-colony forming cell [CFC]), but not in erythrocyte progenitor cells from day 15 fetal liver. Expression of serpin 2A also showed a marked up regulation during the activation of cytotoxic suppressor CD8+ T cells, with a clear lag between the appearance of transcript and detection of protein.
Affiliation:
CRC Department of Experimental Haematology, Paterson Institute of Cancer Research, Christie Hospital, Manchester, UK.
Citation:
Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells. 1997, 89 (1):108-18 Blood
Journal:
Blood
Issue Date:
1-Jan-1997
URI:
http://hdl.handle.net/10541/94933
PubMed ID:
8978283
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHampson, Ian Nen
dc.contributor.authorHampson, Lynneen
dc.contributor.authorPinkoski, Men
dc.contributor.authorCross, Men
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorBleackley, R Cen
dc.contributor.authorAtkinson, Een
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-03-24T15:52:47Z-
dc.date.available2010-03-24T15:52:47Z-
dc.date.issued1997-01-01-
dc.identifier.citationIdentification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells. 1997, 89 (1):108-18 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid8978283-
dc.identifier.urihttp://hdl.handle.net/10541/94933-
dc.description.abstractWe have identified a gene that has a high level of mRNA expression in undifferentiated, multipotential hematopoietic cells (FDCP-Mix) and that downregulates both transcript and protein, as these cells are induced to differentiate into mature myeloid cells. Sequence analysis of this gene has identified it as a serine protease inhibitor EB22/3 (serpin 2A). Constitutive expression of serpin 2A in FDCP-Mix cells was associated with an increase in the clonogenic potential of the cells and with a delay in the appearance of fully mature cells in cultures undergoing granulocyte macrophage differentiation when compared with control cells. Serpin 2A was also found to be expressed in bone marrow-derived bipotent granulocyte macrophage progenitor cells (GM-colony forming cell [CFC]), but not in erythrocyte progenitor cells from day 15 fetal liver. Expression of serpin 2A also showed a marked up regulation during the activation of cytotoxic suppressor CD8+ T cells, with a clear lag between the appearance of transcript and detection of protein.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshCD8-Positive T-Lymphocytes-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Line-
dc.subject.meshGene Expression Regulation-
dc.subject.meshGenes-
dc.subject.meshGenetic Vectors-
dc.subject.meshGranulocytes-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshLiver-
dc.subject.meshLymphocyte Activation-
dc.subject.meshMacrophages-
dc.subject.meshMice-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshSerpins-
dc.subject.meshTransfection-
dc.titleIdentification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Experimental Haematology, Paterson Institute of Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalBlooden

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