A chromosome 14q11/TCR alpha/delta specific yeast artificial chromosome improves the detection rate and characterization of chromosome abnormalities in T-lymphoproliferative disorders.

2.50
Hdl Handle:
http://hdl.handle.net/10541/94930
Title:
A chromosome 14q11/TCR alpha/delta specific yeast artificial chromosome improves the detection rate and characterization of chromosome abnormalities in T-lymphoproliferative disorders.
Authors:
Rack, K A; Cornélis, F; Radford-Weiss, I; Bernheim, A; Harrison, Christine J; Hermine, O; Prieur, M; Vekemans, M; Macintyre, E A
Abstract:
The rate of detection of chromosome abnormalities in T-cell proliferations is lower than that observed in B-cell malignancies. The former frequently involve the TCR alpha/delta locus at chromosome band 14q11. We have identified a YAC encompassing 70% of the TCR alpha/delta locus, which has been used as a fluorescence in situ hybridization probe to detect chromosome rearrangements involving 14q11, both at metaphase and within interphase nuclei, in patients with a variety of T-lymphoproliferative disorders. Its use allowed detection of previously unsuspected TCR alpha/delta rearrangements in 4/13 (30%) immature T-lineage acute leukemias, including two t(10;14) and 2 minor inversion 14s. It also clarified interpretation of complex chromosome 14 abnormalities in mature T-cell proliferations (T-prolymphocytic leukemia and ataxia telangiectasia). Use of this probe will aid the detection and characterization of abnormalities involving the TCR alpha/delta locus, particularly in cases with normal or complex karyotypes and in those proliferations for which mitoses are difficult to obtain.
Affiliation:
Department of Hematology, Hopital Necker Enfants Malades, Paris, France.
Citation:
A chromosome 14q11/TCR alpha/delta specific yeast artificial chromosome improves the detection rate and characterization of chromosome abnormalities in T-lymphoproliferative disorders. 1997, 90 (3):1233-40 Blood
Journal:
Blood
Issue Date:
1-Aug-1997
URI:
http://hdl.handle.net/10541/94930
PubMed ID:
9242557
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorRack, K Aen
dc.contributor.authorCornélis, Fen
dc.contributor.authorRadford-Weiss, Ien
dc.contributor.authorBernheim, Aen
dc.contributor.authorHarrison, Christine Jen
dc.contributor.authorHermine, Oen
dc.contributor.authorPrieur, Men
dc.contributor.authorVekemans, Men
dc.contributor.authorMacintyre, E Aen
dc.date.accessioned2010-03-24T16:19:43Z-
dc.date.available2010-03-24T16:19:43Z-
dc.date.issued1997-08-01-
dc.identifier.citationA chromosome 14q11/TCR alpha/delta specific yeast artificial chromosome improves the detection rate and characterization of chromosome abnormalities in T-lymphoproliferative disorders. 1997, 90 (3):1233-40 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid9242557-
dc.identifier.urihttp://hdl.handle.net/10541/94930-
dc.description.abstractThe rate of detection of chromosome abnormalities in T-cell proliferations is lower than that observed in B-cell malignancies. The former frequently involve the TCR alpha/delta locus at chromosome band 14q11. We have identified a YAC encompassing 70% of the TCR alpha/delta locus, which has been used as a fluorescence in situ hybridization probe to detect chromosome rearrangements involving 14q11, both at metaphase and within interphase nuclei, in patients with a variety of T-lymphoproliferative disorders. Its use allowed detection of previously unsuspected TCR alpha/delta rearrangements in 4/13 (30%) immature T-lineage acute leukemias, including two t(10;14) and 2 minor inversion 14s. It also clarified interpretation of complex chromosome 14 abnormalities in mature T-cell proliferations (T-prolymphocytic leukemia and ataxia telangiectasia). Use of this probe will aid the detection and characterization of abnormalities involving the TCR alpha/delta locus, particularly in cases with normal or complex karyotypes and in those proliferations for which mitoses are difficult to obtain.en
dc.language.isoenen
dc.subjectT-Cell Leukaemiaen
dc.subjectT-Cell Lymphomaen
dc.subjectCancerous Stem Cellsen
dc.subject.meshChromosome Aberrations-
dc.subject.meshChromosome Inversion-
dc.subject.meshChromosomes, Artificial, Yeast-
dc.subject.meshChromosomes, Human, Pair 14-
dc.subject.meshClone Cells-
dc.subject.meshDNA Probes-
dc.subject.meshDNA, Recombinant-
dc.subject.meshFemale-
dc.subject.meshGene Rearrangement, alpha-Chain T-Cell Antigen Receptor-
dc.subject.meshGene Rearrangement, delta-Chain T-Cell Antigen Receptor-
dc.subject.meshHumans-
dc.subject.meshImmunophenotyping-
dc.subject.meshIn Situ Hybridization, Fluorescence-
dc.subject.meshInterphase-
dc.subject.meshLeukemia, T-Cell-
dc.subject.meshLymphoma, T-Cell-
dc.subject.meshMale-
dc.subject.meshMetaphase-
dc.subject.meshNeoplastic Stem Cells-
dc.subject.meshReceptors, Antigen, T-Cell, alpha-beta-
dc.subject.meshReceptors, Antigen, T-Cell, gamma-delta-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshT-Lymphocytes-
dc.subject.meshTranslocation, Genetic-
dc.titleA chromosome 14q11/TCR alpha/delta specific yeast artificial chromosome improves the detection rate and characterization of chromosome abnormalities in T-lymphoproliferative disorders.en
dc.typeArticleen
dc.contributor.departmentDepartment of Hematology, Hopital Necker Enfants Malades, Paris, France.en
dc.identifier.journalBlooden

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