SYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/94906
Title:
SYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells.
Authors:
Kiltie, Anne E; Ryan, Anderson J
Abstract:
Pulsed field gel electrophoresis (PFGE) is widely used to measure DNA double strand breaks (dsb). The DNA of cultured cells can be prelabelled with radioactivity, which helps greatly in detection and quantitation of DNA dsb. However, this approach cannot be used with non-cycling cells from biopsy material. We describe a method which uses SYBR Green I to stain DNA in dried agarose gels. DNA is detected and analysed using readily available camera equipment and image analysis software. This method is as sensitive as [3H]thymidine prelabelling of cells and allows DNA dsb to be measured simply and economically in non-cycling cells.
Affiliation:
Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester M20 4BX, UK.
Citation:
SYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells. 1997, 25 (14):2945-6 Nucleic Acids Res.
Journal:
Nucleic Acids Research
Issue Date:
15-Jul-1997
URI:
http://hdl.handle.net/10541/94906
DOI:
10.1093/nar/25.14.2945
PubMed ID:
9207049
Type:
Article
Language:
en
ISSN:
0305-1048
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorKiltie, Anne Een
dc.contributor.authorRyan, Anderson Jen
dc.date.accessioned2010-03-24T15:27:52Z-
dc.date.available2010-03-24T15:27:52Z-
dc.date.issued1997-07-15-
dc.identifier.citationSYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells. 1997, 25 (14):2945-6 Nucleic Acids Res.en
dc.identifier.issn0305-1048-
dc.identifier.pmid9207049-
dc.identifier.doi10.1093/nar/25.14.2945-
dc.identifier.urihttp://hdl.handle.net/10541/94906-
dc.description.abstractPulsed field gel electrophoresis (PFGE) is widely used to measure DNA double strand breaks (dsb). The DNA of cultured cells can be prelabelled with radioactivity, which helps greatly in detection and quantitation of DNA dsb. However, this approach cannot be used with non-cycling cells from biopsy material. We describe a method which uses SYBR Green I to stain DNA in dried agarose gels. DNA is detected and analysed using readily available camera equipment and image analysis software. This method is as sensitive as [3H]thymidine prelabelling of cells and allows DNA dsb to be measured simply and economically in non-cycling cells.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBacteriophage lambda-
dc.subject.meshDNA-
dc.subject.meshDNA Damage-
dc.subject.meshDNA, Viral-
dc.subject.meshElectrophoresis, Gel, Pulsed-Field-
dc.subject.meshFluorescent Dyes-
dc.subject.meshMammals-
dc.subject.meshOrganic Chemicals-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshSepharose-
dc.subject.meshStaining and Labeling-
dc.titleSYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester M20 4BX, UK.en
dc.identifier.journalNucleic Acids Researchen
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