A simple clinical method for the preparation of improved cervical smears-approximating to monolayers.

2.50
Hdl Handle:
http://hdl.handle.net/10541/94821
Title:
A simple clinical method for the preparation of improved cervical smears-approximating to monolayers.
Authors:
Steven, F S; Palcic, B; Sin, J; Desai, Mina
Abstract:
Conventionally prepared cervical smears contain multilayers of cells deposited within strands of mucin. The present study is concerned with chemical reduction of disulphide bonds in the mucin leading to depolymerisation prior to forming a smear in the conventional manner. The resultant distribution of cells on the slide is similar to that obtained by machines designed to produce monolayers of cells. These monolayers have been developed for use in automated analysis of cervical smears and sputum samples. This new technique does not interfere with conventional PAP analysis of dyskaryotic cells nor does it interfere with the fluorescent location of such cells of cytological interest.
Affiliation:
Division of Biochemistry, School of Biological Sciences, University of Manchester, U.K.
Citation:
A simple clinical method for the preparation of improved cervical smears-approximating to monolayers., 17 (1B):629-32 Anticancer Res.
Journal:
Anticancer Research
Issue Date:
1997
URI:
http://hdl.handle.net/10541/94821
PubMed ID:
9066591
Type:
Article
Language:
en
ISSN:
0250-7005
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorSteven, F Sen
dc.contributor.authorPalcic, Ben
dc.contributor.authorSin, Jen
dc.contributor.authorDesai, Minaen
dc.date.accessioned2010-03-24T10:18:27Z-
dc.date.available2010-03-24T10:18:27Z-
dc.date.issued1997-
dc.identifier.citationA simple clinical method for the preparation of improved cervical smears-approximating to monolayers., 17 (1B):629-32 Anticancer Res.en
dc.identifier.issn0250-7005-
dc.identifier.pmid9066591-
dc.identifier.urihttp://hdl.handle.net/10541/94821-
dc.description.abstractConventionally prepared cervical smears contain multilayers of cells deposited within strands of mucin. The present study is concerned with chemical reduction of disulphide bonds in the mucin leading to depolymerisation prior to forming a smear in the conventional manner. The resultant distribution of cells on the slide is similar to that obtained by machines designed to produce monolayers of cells. These monolayers have been developed for use in automated analysis of cervical smears and sputum samples. This new technique does not interfere with conventional PAP analysis of dyskaryotic cells nor does it interfere with the fluorescent location of such cells of cytological interest.en
dc.language.isoenen
dc.subject.meshDithiothreitol-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMucins-
dc.subject.meshSulfhydryl Reagents-
dc.subject.meshVaginal Smears-
dc.titleA simple clinical method for the preparation of improved cervical smears-approximating to monolayers.en
dc.typeArticleen
dc.contributor.departmentDivision of Biochemistry, School of Biological Sciences, University of Manchester, U.K.en
dc.identifier.journalAnticancer Researchen

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