A comparison of three procedures for the detection of Bence-Jones proteinuria.

2.50
Hdl Handle:
http://hdl.handle.net/10541/94819
Title:
A comparison of three procedures for the detection of Bence-Jones proteinuria.
Authors:
Wong, W K; Wieringa, Gilbert E; Stec, Z; Russell, J; Cooke, S; Keevil, Brian G; Lockhart, S
Abstract:
A traditional electrophoretic procedure for detection of Bence-Jones proteinuria, employing Amido black stain on 200-fold concentrated urine, has been compared to two procedures employing highly sensitive protein stains not requiring prior urine concentration. All three procedures were carried out on 80 random urine samples screened for Bence-Jones proteinuria and 10 samples were provided by patients attending a myeloma clinic. A new procedure employing modified Coomassie brilliant blue stain on unconcentrated urine showed comparable sensitivity to the established procedure (82% versus 88%, respectively) and specificity (77% versus 74%, respectively), when assessed against immunofixation as a reference method. However, the new method is considerably quicker and cheaper. A second method, employing Gold stain, showed enhanced sensitivity (94% versus 88% for Amido black) but lower specificity (62% versus 74% for Amido black). However, this method is labour intensive and relatively expensive. Our data suggest that the procedure employing modified Coomassie brilliant blue may be a suitable alternative to the traditional procedure commonly used in many clinical laboratories.
Affiliation:
Department of Biochemistry, Christie Hospital NHS Trust, Manchester UK.
Citation:
A comparison of three procedures for the detection of Bence-Jones proteinuria. 1997, 34 ( Pt 4):371-4 Ann. Clin. Biochem.
Journal:
Annals of Clinical Biochemistry
Issue Date:
Jul-1997
URI:
http://hdl.handle.net/10541/94819
PubMed ID:
9247668
Type:
Article
Language:
en
ISSN:
0004-5632
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorWong, W Ken
dc.contributor.authorWieringa, Gilbert Een
dc.contributor.authorStec, Zen
dc.contributor.authorRussell, Jen
dc.contributor.authorCooke, Sen
dc.contributor.authorKeevil, Brian Gen
dc.contributor.authorLockhart, Sen
dc.date.accessioned2010-03-24T10:10:31Z-
dc.date.available2010-03-24T10:10:31Z-
dc.date.issued1997-07-
dc.identifier.citationA comparison of three procedures for the detection of Bence-Jones proteinuria. 1997, 34 ( Pt 4):371-4 Ann. Clin. Biochem.en
dc.identifier.issn0004-5632-
dc.identifier.pmid9247668-
dc.identifier.urihttp://hdl.handle.net/10541/94819-
dc.description.abstractA traditional electrophoretic procedure for detection of Bence-Jones proteinuria, employing Amido black stain on 200-fold concentrated urine, has been compared to two procedures employing highly sensitive protein stains not requiring prior urine concentration. All three procedures were carried out on 80 random urine samples screened for Bence-Jones proteinuria and 10 samples were provided by patients attending a myeloma clinic. A new procedure employing modified Coomassie brilliant blue stain on unconcentrated urine showed comparable sensitivity to the established procedure (82% versus 88%, respectively) and specificity (77% versus 74%, respectively), when assessed against immunofixation as a reference method. However, the new method is considerably quicker and cheaper. A second method, employing Gold stain, showed enhanced sensitivity (94% versus 88% for Amido black) but lower specificity (62% versus 74% for Amido black). However, this method is labour intensive and relatively expensive. Our data suggest that the procedure employing modified Coomassie brilliant blue may be a suitable alternative to the traditional procedure commonly used in many clinical laboratories.en
dc.language.isoenen
dc.subject.meshAmido Black-
dc.subject.meshAmyloidosis-
dc.subject.meshBence Jones Protein-
dc.subject.meshColoring Agents-
dc.subject.meshElectrophoresis, Agar Gel-
dc.subject.meshGold-
dc.subject.meshHumans-
dc.subject.meshImmunologic Techniques-
dc.subject.meshIndicators and Reagents-
dc.subject.meshMultiple Myeloma-
dc.subject.meshProteinuria-
dc.subject.meshReagent Kits, Diagnostic-
dc.subject.meshRosaniline Dyes-
dc.subject.meshSensitivity and Specificity-
dc.titleA comparison of three procedures for the detection of Bence-Jones proteinuria.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biochemistry, Christie Hospital NHS Trust, Manchester UK.en
dc.identifier.journalAnnals of Clinical Biochemistryen
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