Relationship between growth hormone (GH) status, serum leptin and body composition in healthy and GH deficient elderly subjects.

2.50
Hdl Handle:
http://hdl.handle.net/10541/94774
Title:
Relationship between growth hormone (GH) status, serum leptin and body composition in healthy and GH deficient elderly subjects.
Authors:
Gill, M S; Toogood, Andy; O'Neill, Paul A; Adams, J E; Thorner, M O; Shalet, Stephen M; Clayton, Peter E
Abstract:
OBJECTIVE: Growth hormone (GH) secretion declines with age and is affected by body composition. The signal that mediates the latter relationship remains III-defined. Leptin, the protein product of the adipocyte specific ob gene, is thought to accurately reflect fat mass and could therefore be a candidate to influence GH secretion. We have therefore investigated the relationship between GH status, leptin and body composition in normal and GH-deficient elderly subjects. DESIGN: GH Secretion was assessed by 20-minute sampling over 24 hours and serum leptin concentrations were measured in a single morning, fasted sample. PATIENTS: Twenty-one GH deficient elderly patients (61-83 years) and 22 gender- and BMI-matched controls (61-88 years). MEASUREMENTS: Body composition was assessed by dual-energy X-ray absorptiometry (DEXA). GH was measured in an ultrasensitive chemiluminescent assay and serum leptin was determined by radio-immunoassay. RESULTS: Leptin was correlated with percentage body fat in both sexes (male r = 0.75, female r = 0.89, both P < 0.001). Male patients had increased fat mass (FM) (P < 0.01) and leptin concentrations (P < 0.05) but similar lean mass (LM) compared with controls. However, leptin concentration per unit FM was identical in both groups (P = 0.3). In contrast, female patients had lower LM (P < 0.05) but similar FM to controls, yet their leptin concentration per unit FM was twice that of the controls (P < 0.05). In multiple linear regression (MLR) leptin was determined positively by FM and negatively by LM (controls r2 = 76%; patients r2 = 73%, both P < 0.0001). When controlled for gender, GH secretion in the controls was correlated negatively with leptin (r = -0.68, P < 0.01) and negatively with percentage body fat (r = -0.73, P < 0.01). In MLR, using leptin as a marker of body composition, 66% of the variability in GH secretion in the controls could be explained by gender (38%) and by leptin (28%). CONCLUSIONS: Both decreased lean mass and increased fat mass raise serum leptin concentrations in normal and growth hormone-deficient elderly subjects. Leptin is therefore a marker of body composition rather than fat mass alone. The influence of body composition on growth hormone secretion in the elderly may be mediated through leptin, acting as a peripheral signal from adipose tissue to decrease GH secretion.
Affiliation:
Department of Medicine, University of Manchester, UK.
Citation:
Relationship between growth hormone (GH) status, serum leptin and body composition in healthy and GH deficient elderly subjects. 1997, 47 (2):161-7 Clin. Endocrinol.
Journal:
Clinical Endocrinology
Issue Date:
Aug-1997
URI:
http://hdl.handle.net/10541/94774
DOI:
10.1046/j.1365-2265.1997.2291056.x
PubMed ID:
9302388
Type:
Article
Language:
en
ISSN:
0300-0664
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorGill, M Sen
dc.contributor.authorToogood, Andyen
dc.contributor.authorO'Neill, Paul Aen
dc.contributor.authorAdams, J Een
dc.contributor.authorThorner, M Oen
dc.contributor.authorShalet, Stephen Men
dc.contributor.authorClayton, Peter Een
dc.date.accessioned2010-03-23T17:10:29Z-
dc.date.available2010-03-23T17:10:29Z-
dc.date.issued1997-08-
dc.identifier.citationRelationship between growth hormone (GH) status, serum leptin and body composition in healthy and GH deficient elderly subjects. 1997, 47 (2):161-7 Clin. Endocrinol.en
dc.identifier.issn0300-0664-
dc.identifier.pmid9302388-
dc.identifier.doi10.1046/j.1365-2265.1997.2291056.x-
dc.identifier.urihttp://hdl.handle.net/10541/94774-
dc.description.abstractOBJECTIVE: Growth hormone (GH) secretion declines with age and is affected by body composition. The signal that mediates the latter relationship remains III-defined. Leptin, the protein product of the adipocyte specific ob gene, is thought to accurately reflect fat mass and could therefore be a candidate to influence GH secretion. We have therefore investigated the relationship between GH status, leptin and body composition in normal and GH-deficient elderly subjects. DESIGN: GH Secretion was assessed by 20-minute sampling over 24 hours and serum leptin concentrations were measured in a single morning, fasted sample. PATIENTS: Twenty-one GH deficient elderly patients (61-83 years) and 22 gender- and BMI-matched controls (61-88 years). MEASUREMENTS: Body composition was assessed by dual-energy X-ray absorptiometry (DEXA). GH was measured in an ultrasensitive chemiluminescent assay and serum leptin was determined by radio-immunoassay. RESULTS: Leptin was correlated with percentage body fat in both sexes (male r = 0.75, female r = 0.89, both P < 0.001). Male patients had increased fat mass (FM) (P < 0.01) and leptin concentrations (P < 0.05) but similar lean mass (LM) compared with controls. However, leptin concentration per unit FM was identical in both groups (P = 0.3). In contrast, female patients had lower LM (P < 0.05) but similar FM to controls, yet their leptin concentration per unit FM was twice that of the controls (P < 0.05). In multiple linear regression (MLR) leptin was determined positively by FM and negatively by LM (controls r2 = 76%; patients r2 = 73%, both P < 0.0001). When controlled for gender, GH secretion in the controls was correlated negatively with leptin (r = -0.68, P < 0.01) and negatively with percentage body fat (r = -0.73, P < 0.01). In MLR, using leptin as a marker of body composition, 66% of the variability in GH secretion in the controls could be explained by gender (38%) and by leptin (28%). CONCLUSIONS: Both decreased lean mass and increased fat mass raise serum leptin concentrations in normal and growth hormone-deficient elderly subjects. Leptin is therefore a marker of body composition rather than fat mass alone. The influence of body composition on growth hormone secretion in the elderly may be mediated through leptin, acting as a peripheral signal from adipose tissue to decrease GH secretion.en
dc.language.isoenen
dc.subject.meshAbsorptiometry, Photon-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshBody Composition-
dc.subject.meshCohort Studies-
dc.subject.meshFemale-
dc.subject.meshGrowth Hormone-
dc.subject.meshHumans-
dc.subject.meshLeptin-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshProteins-
dc.subject.meshRegression Analysis-
dc.subject.meshSex Factors-
dc.titleRelationship between growth hormone (GH) status, serum leptin and body composition in healthy and GH deficient elderly subjects.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine, University of Manchester, UK.en
dc.identifier.journalClinical Endocrinologyen

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