Abnormal expression of CCND1 and RB1 in resection margin epithelia of lung cancer patients.

2.50
Hdl Handle:
http://hdl.handle.net/10541/94747
Title:
Abnormal expression of CCND1 and RB1 in resection margin epithelia of lung cancer patients.
Authors:
Betticher, Daniel C; Heighway, Jim; Thatcher, Nick; Hasleton, Philip S
Abstract:
Tumours develop through the accumulation of genetic alterations associated with a progressive increase of the malignant phenotype. In lung cancer, chronic exposure of bronchial epithelium to carcinogens in cigarette smoke may lead to multiple dysplastic and hyperplastic lesions scattered throughout the tracheobronchial tree. Little is known about the genetic alterations in such lesions. This study was carried out to examine cyclin D1 (CCND1) and retinoblastoma (RB1) gene expression in the bronchial epithelium of patients with lung cancer. Lung tumours and their corresponding tumour-free resection margins from 33 patients who underwent resection of non-small-cell lung cancer (NSCLC) were examined by immunostaining with monoclonal antibodies against cyclin D1 (DCS-6; Novocastra) and pRb (NCL Rb-1; Novocastra). Examination of the resection margins revealed four carcinomas in situ, 19 hyperplasias and ten sections showing apparently normal bronchial epithelium. A control group of patients, without lung tumours and who had never smoked, revealed no or weak cyclin D1 and positive pRb staining within bronchial epithelia. Increased cyclin D1 and diminished pRb expression were found in 76% (n = 25) and 27% (n = 9) of the resection margins respectively, and in 12% (n = 4) both cyclin D1 and pRb expression were altered. In the corresponding tumours, 48% (n = 16) were normal, while altered expression was found for cyclin D1 in 33% (n = 11), pRb in 27% (n = 9) and both in 9% (n = 3) of cases. It appears that altered expression of cyclin D1 and pRb is an early event in NSCLC development in almost half of cases analysed. Further investigations are needed to determine the significance of immunostaining of bronchial specimens in individuals at risk of lung cancer, with the possibility that the observations are of importance in the early diagnosis of NSCLC.
Affiliation:
CRC Department of Med. Oncology, Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
Abnormal expression of CCND1 and RB1 in resection margin epithelia of lung cancer patients. 1997, 75 (12):1761-8 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
1997
URI:
http://hdl.handle.net/10541/94747
PubMed ID:
9192978
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBetticher, Daniel Cen
dc.contributor.authorHeighway, Jimen
dc.contributor.authorThatcher, Nicken
dc.contributor.authorHasleton, Philip Sen
dc.date.accessioned2010-03-23T16:38:54Z-
dc.date.available2010-03-23T16:38:54Z-
dc.date.issued1997-
dc.identifier.citationAbnormal expression of CCND1 and RB1 in resection margin epithelia of lung cancer patients. 1997, 75 (12):1761-8 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid9192978-
dc.identifier.urihttp://hdl.handle.net/10541/94747-
dc.description.abstractTumours develop through the accumulation of genetic alterations associated with a progressive increase of the malignant phenotype. In lung cancer, chronic exposure of bronchial epithelium to carcinogens in cigarette smoke may lead to multiple dysplastic and hyperplastic lesions scattered throughout the tracheobronchial tree. Little is known about the genetic alterations in such lesions. This study was carried out to examine cyclin D1 (CCND1) and retinoblastoma (RB1) gene expression in the bronchial epithelium of patients with lung cancer. Lung tumours and their corresponding tumour-free resection margins from 33 patients who underwent resection of non-small-cell lung cancer (NSCLC) were examined by immunostaining with monoclonal antibodies against cyclin D1 (DCS-6; Novocastra) and pRb (NCL Rb-1; Novocastra). Examination of the resection margins revealed four carcinomas in situ, 19 hyperplasias and ten sections showing apparently normal bronchial epithelium. A control group of patients, without lung tumours and who had never smoked, revealed no or weak cyclin D1 and positive pRb staining within bronchial epithelia. Increased cyclin D1 and diminished pRb expression were found in 76% (n = 25) and 27% (n = 9) of the resection margins respectively, and in 12% (n = 4) both cyclin D1 and pRb expression were altered. In the corresponding tumours, 48% (n = 16) were normal, while altered expression was found for cyclin D1 in 33% (n = 11), pRb in 27% (n = 9) and both in 9% (n = 3) of cases. It appears that altered expression of cyclin D1 and pRb is an early event in NSCLC development in almost half of cases analysed. Further investigations are needed to determine the significance of immunostaining of bronchial specimens in individuals at risk of lung cancer, with the possibility that the observations are of importance in the early diagnosis of NSCLC.en
dc.language.isoenen
dc.subjectTumour Suppressor Proteinsen
dc.subjectBiological Tumour Markersen
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshCarcinoma in Situ-
dc.subject.meshCarcinoma, Non-Small-Cell Lung-
dc.subject.meshCyclin D1-
dc.subject.meshCyclins-
dc.subject.meshData Interpretation, Statistical-
dc.subject.meshEpithelium-
dc.subject.meshFemale-
dc.subject.meshGenes, Retinoblastoma-
dc.subject.meshHumans-
dc.subject.meshHyperplasia-
dc.subject.meshImmunohistochemistry-
dc.subject.meshLung-
dc.subject.meshLung Neoplasms-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshOncogene Proteins-
dc.subject.meshPrognosis-
dc.subject.meshStaining and Labeling-
dc.subject.meshTumor Markers, Biological-
dc.titleAbnormal expression of CCND1 and RB1 in resection margin epithelia of lung cancer patients.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Med. Oncology, Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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