A putative role for platelet-derived growth factor in angiogenesis and neuroprotection after ischemic stroke in humans.

2.50
Hdl Handle:
http://hdl.handle.net/10541/94698
Title:
A putative role for platelet-derived growth factor in angiogenesis and neuroprotection after ischemic stroke in humans.
Authors:
Krupinski, J; Issa, R; Bujny, T; Slevin, M; Kumar, P; Kumar, Shant; Kaluza, J
Abstract:
BACKGROUND AND PURPOSE: Growth factors control two important processes in infarcted tissue, ie, angiogenesis and gliosis. We recently reported that transforming growth factor-beta1 (TGF-beta1) might be involved in angiogenesis after ischemic stroke in humans; here we present data of an extensive study on platelet-derived growth factor (PDGF) and its receptors. METHODS: We studied brain samples from patients who suffered from ischemic stroke for the expression of mRNA encoding PDGF-A, PDGF-B, and PDGF receptors (PDGF-R). Proteins were examined by Western blotting and immunohistochemistry using the antibodies to PDGF-AB, PDGF-BB, PDGF-R alpha, and PDGF-R beta. RESULTS: At the mRNA level, PDGF-A and PDGF-B were expressed mainly in neurons in penumbra. PDGF-R mRNA was strongly expressed in some astrocytes but mainly in type III/IV neurons in infarct and penumbra. The least expression was seen in the contralateral hemisphere (P<.001). In contrast, both PDGF-AB and PDGF-BB immunoreactive products were present in most cell types: PDGF-R alpha and PDGF-R beta mainly on neurons, and PDGF-R beta on some endothelial cells, with less staining of all the isoforms in the contralateral hemisphere. On Western blots, PDGF-AB and -BB were expressed more within white matter than gray matter of infarct/penumbra, whereas both isoforms of receptor were expressed mainly in gray matter compared with contralateral hemisphere. There was no or very weak expression of the receptor in white matter. CONCLUSIONS: PDGF proteins are highly expressed in white matter, suggesting that PDGF may exert its function in white matter participating either in regeneration of damaged axons or in glial scar formation. PDGF-BB and its receptor expressed on microvessel endothelial cells might be involved in angiogenesis after stroke. Thus, PDGF is likely to be angiogenic and neuroprotective in stroke.
Affiliation:
Department of Biological Sciences, Manchester Metropolitan University, UK.
Citation:
A putative role for platelet-derived growth factor in angiogenesis and neuroprotection after ischemic stroke in humans. 1997, 28 (3):564-73 Stroke
Journal:
Stroke
Issue Date:
Mar-1997
URI:
http://hdl.handle.net/10541/94698
PubMed ID:
9056612
Type:
Article
Language:
en
ISSN:
0039-2499
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorKrupinski, Jen
dc.contributor.authorIssa, Ren
dc.contributor.authorBujny, Ten
dc.contributor.authorSlevin, Men
dc.contributor.authorKumar, Pen
dc.contributor.authorKumar, Shanten
dc.contributor.authorKaluza, Jen
dc.date.accessioned2010-03-23T13:27:12Z-
dc.date.available2010-03-23T13:27:12Z-
dc.date.issued1997-03-
dc.identifier.citationA putative role for platelet-derived growth factor in angiogenesis and neuroprotection after ischemic stroke in humans. 1997, 28 (3):564-73 Strokeen
dc.identifier.issn0039-2499-
dc.identifier.pmid9056612-
dc.identifier.urihttp://hdl.handle.net/10541/94698-
dc.description.abstractBACKGROUND AND PURPOSE: Growth factors control two important processes in infarcted tissue, ie, angiogenesis and gliosis. We recently reported that transforming growth factor-beta1 (TGF-beta1) might be involved in angiogenesis after ischemic stroke in humans; here we present data of an extensive study on platelet-derived growth factor (PDGF) and its receptors. METHODS: We studied brain samples from patients who suffered from ischemic stroke for the expression of mRNA encoding PDGF-A, PDGF-B, and PDGF receptors (PDGF-R). Proteins were examined by Western blotting and immunohistochemistry using the antibodies to PDGF-AB, PDGF-BB, PDGF-R alpha, and PDGF-R beta. RESULTS: At the mRNA level, PDGF-A and PDGF-B were expressed mainly in neurons in penumbra. PDGF-R mRNA was strongly expressed in some astrocytes but mainly in type III/IV neurons in infarct and penumbra. The least expression was seen in the contralateral hemisphere (P<.001). In contrast, both PDGF-AB and PDGF-BB immunoreactive products were present in most cell types: PDGF-R alpha and PDGF-R beta mainly on neurons, and PDGF-R beta on some endothelial cells, with less staining of all the isoforms in the contralateral hemisphere. On Western blots, PDGF-AB and -BB were expressed more within white matter than gray matter of infarct/penumbra, whereas both isoforms of receptor were expressed mainly in gray matter compared with contralateral hemisphere. There was no or very weak expression of the receptor in white matter. CONCLUSIONS: PDGF proteins are highly expressed in white matter, suggesting that PDGF may exert its function in white matter participating either in regeneration of damaged axons or in glial scar formation. PDGF-BB and its receptor expressed on microvessel endothelial cells might be involved in angiogenesis after stroke. Thus, PDGF is likely to be angiogenic and neuroprotective in stroke.en
dc.language.isoenen
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshAntibody Specificity-
dc.subject.meshBlotting, Western-
dc.subject.meshBrain-
dc.subject.meshBrain Chemistry-
dc.subject.meshBrain Ischemia-
dc.subject.meshCell Communication-
dc.subject.meshCerebral Infarction-
dc.subject.meshCerebrovascular Disorders-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshIn Situ Hybridization-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshNeovascularization, Physiologic-
dc.subject.meshNeuroglia-
dc.subject.meshNeurons-
dc.subject.meshPlatelet-Derived Growth Factor-
dc.subject.meshRNA, Messenger-
dc.subject.meshReceptors, Platelet-Derived Growth Factor-
dc.titleA putative role for platelet-derived growth factor in angiogenesis and neuroprotection after ischemic stroke in humans.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biological Sciences, Manchester Metropolitan University, UK.en
dc.identifier.journalStrokeen

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