Insulin-like growth factor II and PAX3-FKHR cooperate in the oncogenesis of rhabdomyosarcoma.

2.50
Hdl Handle:
http://hdl.handle.net/10541/93436
Title:
Insulin-like growth factor II and PAX3-FKHR cooperate in the oncogenesis of rhabdomyosarcoma.
Authors:
Wang, W; Kumar, P; Wang, W; Epstein, J; Helman, L; Moore, James V; Kumar, Shant
Abstract:
The mouse myoblast C2C12 cell line transfected singly with cDNA for Pax-3, PAX3-FKHR, or insulin-like growth factor (IGF) II or cotransfected with IGF-II plus Pax-3 or with IGF-II plus PAX3-FKHR genes showed an altered morphology, a lack of differentiation, and higher proliferation rates in vitro. On s.c. injection into nude mice, tumors grew from transfected cell lines but not from cells transfected with the empty vector. Tumors derived from IGF-II/PAX3-FKHR- and IGF-II-transfected cells grew most rapidly. Cotransfection of IGF-II plus Pax-3 induced tumors comprised highly differentiated striated muscle cells; Pax-3, PAX3-FKHR, or IGF-II transfection produced tumors at varying stages of differentiation. Tumors derived from IGF-II plus PAX3-FKHR-cotransfected cells were composed of undifferentiated cells. This was the only tumor type to infiltrate the underlying muscle. The most angiogenesis and the least apoptosis were observed in the latter tumors. These results support the hypothesis that PAX3-FKHR interacts with IGF-II to play a critical role in the oncogenesis of rhabdomyosarcoma.
Affiliation:
Manchester Metropolitan University, Manchester, United Kingdom.
Citation:
Insulin-like growth factor II and PAX3-FKHR cooperate in the oncogenesis of rhabdomyosarcoma. 1998, 58 (19):4426-33 Cancer Res.
Journal:
Cancer Research
Issue Date:
1-Oct-1998
URI:
http://hdl.handle.net/10541/93436
PubMed ID:
9766674
Type:
Article
Language:
en
ISSN:
0008-5472
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Wen
dc.contributor.authorKumar, Pen
dc.contributor.authorWang, Wen
dc.contributor.authorEpstein, Jen
dc.contributor.authorHelman, Len
dc.contributor.authorMoore, James Ven
dc.contributor.authorKumar, Shanten
dc.date.accessioned2010-03-02T17:23:43Z-
dc.date.available2010-03-02T17:23:43Z-
dc.date.issued1998-10-01-
dc.identifier.citationInsulin-like growth factor II and PAX3-FKHR cooperate in the oncogenesis of rhabdomyosarcoma. 1998, 58 (19):4426-33 Cancer Res.en
dc.identifier.issn0008-5472-
dc.identifier.pmid9766674-
dc.identifier.urihttp://hdl.handle.net/10541/93436-
dc.description.abstractThe mouse myoblast C2C12 cell line transfected singly with cDNA for Pax-3, PAX3-FKHR, or insulin-like growth factor (IGF) II or cotransfected with IGF-II plus Pax-3 or with IGF-II plus PAX3-FKHR genes showed an altered morphology, a lack of differentiation, and higher proliferation rates in vitro. On s.c. injection into nude mice, tumors grew from transfected cell lines but not from cells transfected with the empty vector. Tumors derived from IGF-II/PAX3-FKHR- and IGF-II-transfected cells grew most rapidly. Cotransfection of IGF-II plus Pax-3 induced tumors comprised highly differentiated striated muscle cells; Pax-3, PAX3-FKHR, or IGF-II transfection produced tumors at varying stages of differentiation. Tumors derived from IGF-II plus PAX3-FKHR-cotransfected cells were composed of undifferentiated cells. This was the only tumor type to infiltrate the underlying muscle. The most angiogenesis and the least apoptosis were observed in the latter tumors. These results support the hypothesis that PAX3-FKHR interacts with IGF-II to play a critical role in the oncogenesis of rhabdomyosarcoma.en
dc.language.isoenen
dc.subjectCancerous Cell Transformationen
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshCell Line-
dc.subject.meshCell Transformation, Neoplastic-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshForkhead Transcription Factors-
dc.subject.meshHumans-
dc.subject.meshInsulin-Like Growth Factor II-
dc.subject.meshKinetics-
dc.subject.meshMice-
dc.subject.meshMice, Nude-
dc.subject.meshMicrocirculation-
dc.subject.meshMuscle, Skeletal-
dc.subject.meshNeovascularization, Pathologic-
dc.subject.meshPaired Box Transcription Factors-
dc.subject.meshRecombinant Fusion Proteins-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshRhabdomyosarcoma-
dc.subject.meshTranscription Factors-
dc.subject.meshTransfection-
dc.titleInsulin-like growth factor II and PAX3-FKHR cooperate in the oncogenesis of rhabdomyosarcoma.en
dc.typeArticleen
dc.contributor.departmentManchester Metropolitan University, Manchester, United Kingdom.en
dc.identifier.journalCancer Researchen
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