Mutation studies in lacI transgenic mice after exposure to radiation or cyclophosphamide.

2.50
Hdl Handle:
http://hdl.handle.net/10541/93093
Title:
Mutation studies in lacI transgenic mice after exposure to radiation or cyclophosphamide.
Authors:
Hoyes, Katherine P; Wadeson, P J; Sharma, Harbans L; Hendry, Jolyon H; Morris, Ian D
Abstract:
We have used the Big Blue lacI transgenic mouse reporter system to investigate mutation induction in the testes, spleen and liver after exposure to an internally incorporated radionuclide, 114mIn, whole body irradiation with 60Co gamma-rays and systemically administered cyclophosphamide. Spontaneous mutation frequencies were 6-17x10(-6). No statistically significant mutation induction was observed in testes or spleen at 35 days after exposure to any test agent, although mutation frequencies tended to be increased (by approximately 1.5-fold) after exposure to 1 Gy gamma-rays. However, liver mutation frequencies were doubled after treatment with 100 mg/kg cyclophosphamide and were elevated by approximately 2.5-fold after systemic administration of 114mIn and 4.5-fold after 1 Gy 60Co gamma-rays. When data from all organs were pooled, mutation frequency was doubled after exposure to 1 Gy gamma-rays, but no other significant increases were observed. These findings support the hypothesis that the lacI transgenic mouse may be relatively inefficient at detecting mutations induced by exposure to ionizing radiation or other agents which produce a spectrum of deletion sizes, including those which are larger than the lacI transgene.
Affiliation:
School of Biological Sciences and Department of Medical Biophysics, University of Manchester, UK. khoyes@picr.man.ac.uk
Citation:
Mutation studies in lacI transgenic mice after exposure to radiation or cyclophosphamide. 1998, 13 (6):607-12 Mutagenesis
Journal:
Mutagenesis
Issue Date:
Nov-1998
URI:
http://hdl.handle.net/10541/93093
PubMed ID:
9862192
Type:
Article
Language:
en
ISSN:
0267-8357
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHoyes, Katherine Pen
dc.contributor.authorWadeson, P Jen
dc.contributor.authorSharma, Harbans Len
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorMorris, Ian Den
dc.date.accessioned2010-02-25T15:49:41Z-
dc.date.available2010-02-25T15:49:41Z-
dc.date.issued1998-11-
dc.identifier.citationMutation studies in lacI transgenic mice after exposure to radiation or cyclophosphamide. 1998, 13 (6):607-12 Mutagenesisen
dc.identifier.issn0267-8357-
dc.identifier.pmid9862192-
dc.identifier.urihttp://hdl.handle.net/10541/93093-
dc.description.abstractWe have used the Big Blue lacI transgenic mouse reporter system to investigate mutation induction in the testes, spleen and liver after exposure to an internally incorporated radionuclide, 114mIn, whole body irradiation with 60Co gamma-rays and systemically administered cyclophosphamide. Spontaneous mutation frequencies were 6-17x10(-6). No statistically significant mutation induction was observed in testes or spleen at 35 days after exposure to any test agent, although mutation frequencies tended to be increased (by approximately 1.5-fold) after exposure to 1 Gy gamma-rays. However, liver mutation frequencies were doubled after treatment with 100 mg/kg cyclophosphamide and were elevated by approximately 2.5-fold after systemic administration of 114mIn and 4.5-fold after 1 Gy 60Co gamma-rays. When data from all organs were pooled, mutation frequency was doubled after exposure to 1 Gy gamma-rays, but no other significant increases were observed. These findings support the hypothesis that the lacI transgenic mouse may be relatively inefficient at detecting mutations induced by exposure to ionizing radiation or other agents which produce a spectrum of deletion sizes, including those which are larger than the lacI transgene.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBacterial Proteins-
dc.subject.meshCyclophosphamide-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshEscherichia coli Proteins-
dc.subject.meshIndium Radioisotopes-
dc.subject.meshLac Repressors-
dc.subject.meshLiver-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshMice, Transgenic-
dc.subject.meshMutagenicity Tests-
dc.subject.meshMutagens-
dc.subject.meshMutation-
dc.subject.meshRepressor Proteins-
dc.subject.meshSpleen-
dc.subject.meshTestis-
dc.titleMutation studies in lacI transgenic mice after exposure to radiation or cyclophosphamide.en
dc.typeArticleen
dc.contributor.departmentSchool of Biological Sciences and Department of Medical Biophysics, University of Manchester, UK. khoyes@picr.man.ac.uken
dc.identifier.journalMutagenesisen

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