The radiosensitivity of human fibroblast cell lines correlates with residual levels of DNA double-strand breaks.

2.50
Hdl Handle:
http://hdl.handle.net/10541/93091
Title:
The radiosensitivity of human fibroblast cell lines correlates with residual levels of DNA double-strand breaks.
Authors:
Zhou, Ping-Kun; Sproston, Anthony R; Marples, Brian; West, Catharine M L; Margison, Geoffrey P; Hendry, Jolyon H
Abstract:
PURPOSE: To study the correlation of residual DNA double-strand breakage after irradiation and cellular radiosensitivity in cells showing marked differences in radiosensitivity. MATERIALS AND METHODS: The levels of DNA double-strand breaks remaining at 4 h after irradiation were measured by graded-voltage gel electrophoresis in fibroblast cell strains derived from seven individuals either with normal radiosensitivity (n = 2), or with genetic abnormalities known to show increased (two ataxia telangiectasia, one scid) or possibly decreased (two Li-Fraumeni family members) sensitivity. RESULTS: The slope of the dose-response curve for DNA breaks remaining unrepaired at 4 h showed a highly significant correlation with cellular radiosensitivity characterized by SF2, alpha, or D (r > or = 0.91, P < 0.001). Hence, this measure of genotoxic damage was predictive of radiation sensitivity for cells affected by a variety of mutations in different damage signalling/repair components. DISCUSSION: This correlation confirms another published study and extends it to cell lines with other genetic defects. The technique may be useful in the development of rapid assays to predict the sensitivity of normal tissues in patients receiving radiotherapy.
Affiliation:
Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
The radiosensitivity of human fibroblast cell lines correlates with residual levels of DNA double-strand breaks. 1998, 47 (3):271-6 Radiother Oncol
Journal:
Radiotherapy and Oncology
Issue Date:
Jun-1998
URI:
http://hdl.handle.net/10541/93091
PubMed ID:
9681890
Type:
Article
Language:
en
ISSN:
0167-8140
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorZhou, Ping-Kunen
dc.contributor.authorSproston, Anthony Ren
dc.contributor.authorMarples, Brianen
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorMargison, Geoffrey Pen
dc.contributor.authorHendry, Jolyon Hen
dc.date.accessioned2010-02-25T16:44:34Z-
dc.date.available2010-02-25T16:44:34Z-
dc.date.issued1998-06-
dc.identifier.citationThe radiosensitivity of human fibroblast cell lines correlates with residual levels of DNA double-strand breaks. 1998, 47 (3):271-6 Radiother Oncolen
dc.identifier.issn0167-8140-
dc.identifier.pmid9681890-
dc.identifier.urihttp://hdl.handle.net/10541/93091-
dc.description.abstractPURPOSE: To study the correlation of residual DNA double-strand breakage after irradiation and cellular radiosensitivity in cells showing marked differences in radiosensitivity. MATERIALS AND METHODS: The levels of DNA double-strand breaks remaining at 4 h after irradiation were measured by graded-voltage gel electrophoresis in fibroblast cell strains derived from seven individuals either with normal radiosensitivity (n = 2), or with genetic abnormalities known to show increased (two ataxia telangiectasia, one scid) or possibly decreased (two Li-Fraumeni family members) sensitivity. RESULTS: The slope of the dose-response curve for DNA breaks remaining unrepaired at 4 h showed a highly significant correlation with cellular radiosensitivity characterized by SF2, alpha, or D (r > or = 0.91, P < 0.001). Hence, this measure of genotoxic damage was predictive of radiation sensitivity for cells affected by a variety of mutations in different damage signalling/repair components. DISCUSSION: This correlation confirms another published study and extends it to cell lines with other genetic defects. The technique may be useful in the development of rapid assays to predict the sensitivity of normal tissues in patients receiving radiotherapy.en
dc.language.isoenen
dc.subject.meshBlotting, Southern-
dc.subject.meshCell Line-
dc.subject.meshCell Survival-
dc.subject.meshDNA-
dc.subject.meshDNA Damage-
dc.subject.meshDNA Probes-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshElectrophoresis, Gel, Pulsed-Field-
dc.subject.meshFibroblasts-
dc.subject.meshHumans-
dc.subject.meshRadiation Tolerance-
dc.subject.meshRadiodermatitis-
dc.titleThe radiosensitivity of human fibroblast cell lines correlates with residual levels of DNA double-strand breaks.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalRadiotherapy and Oncologyen

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