Human melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers.

2.50
Hdl Handle:
http://hdl.handle.net/10541/93090
Title:
Human melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers.
Authors:
Young, A R; Potten, Christopher S; Nikaido, O; Parsons, P G; Boenders, Johathan; Ramsden, Jonathan M; Chadwick, Caroline A
Abstract:
Epidemiology shows a relationship between solar exposure and all types of skin cancer. Understanding the mechanisms of skin cancer requires knowledge of the photomolecular events that occur within the relevant epidermal cell types in vivo. Studies to date have focused on UVR-induced DNA lesions in keratinocytes, the majority epidermal cell population which gives rise to most skin cancers. Malignant melanoma, arising from melanocytes (5%-10% of epidermal cells), accounts for most skin cancer deaths. We report on new techniques to detect DNA photolesions in human epidermal melanocytes in situ. Previously nonexposed buttock skin of volunteers of skin types I/II was exposed to clinically relevant doses of narrow bandwidth UVB (300 nm) and UVA (320 nm, 340 nm, 360 nm) radiation. Biopsies were taken immediately afterwards and processed for routine histology. Microscope sections were prepared and double-stained with fluorescent-tagged monoclonal antibodies for thymine dimers and melanocytes. UVR dose-response curves for dimer levels within melanocyte nuclei were determined by image analysis and compared with dimer levels in adjacent basal cell keratinocytes. Our data show that UVB and UVA readily induce thymine dimers in melanocytes at levels that are comparable with those found in adjacent keratinocytes. This new technique will enable melanocyte specific studies, such as DNA repair kinetics, to be done in vivo.
Affiliation:
Department of Photobiology, St John's Institute for Dermatology, St Thomas Hospital, London, UK.
Citation:
Human melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers. 1998, 111 (6):936-40 J. Invest. Dermatol.
Journal:
The Journal of Investigative Dermatology
Issue Date:
Dec-1998
URI:
http://hdl.handle.net/10541/93090
DOI:
10.1046/j.1523-1747.1998.00435.x
PubMed ID:
9856799
Type:
Article
Language:
en
ISSN:
0022-202X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorYoung, A Ren
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorNikaido, Oen
dc.contributor.authorParsons, P Gen
dc.contributor.authorBoenders, Johathanen
dc.contributor.authorRamsden, Jonathan Men
dc.contributor.authorChadwick, Caroline Aen
dc.date.accessioned2010-02-25T16:25:59Z-
dc.date.available2010-02-25T16:25:59Z-
dc.date.issued1998-12-
dc.identifier.citationHuman melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers. 1998, 111 (6):936-40 J. Invest. Dermatol.en
dc.identifier.issn0022-202X-
dc.identifier.pmid9856799-
dc.identifier.doi10.1046/j.1523-1747.1998.00435.x-
dc.identifier.urihttp://hdl.handle.net/10541/93090-
dc.description.abstractEpidemiology shows a relationship between solar exposure and all types of skin cancer. Understanding the mechanisms of skin cancer requires knowledge of the photomolecular events that occur within the relevant epidermal cell types in vivo. Studies to date have focused on UVR-induced DNA lesions in keratinocytes, the majority epidermal cell population which gives rise to most skin cancers. Malignant melanoma, arising from melanocytes (5%-10% of epidermal cells), accounts for most skin cancer deaths. We report on new techniques to detect DNA photolesions in human epidermal melanocytes in situ. Previously nonexposed buttock skin of volunteers of skin types I/II was exposed to clinically relevant doses of narrow bandwidth UVB (300 nm) and UVA (320 nm, 340 nm, 360 nm) radiation. Biopsies were taken immediately afterwards and processed for routine histology. Microscope sections were prepared and double-stained with fluorescent-tagged monoclonal antibodies for thymine dimers and melanocytes. UVR dose-response curves for dimer levels within melanocyte nuclei were determined by image analysis and compared with dimer levels in adjacent basal cell keratinocytes. Our data show that UVB and UVA readily induce thymine dimers in melanocytes at levels that are comparable with those found in adjacent keratinocytes. This new technique will enable melanocyte specific studies, such as DNA repair kinetics, to be done in vivo.en
dc.language.isoenen
dc.subject.meshAdult-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshDNA Repair-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshFluorescence-
dc.subject.meshHumans-
dc.subject.meshKeratinocytes-
dc.subject.meshLinear Models-
dc.subject.meshMelanocytes-
dc.subject.meshPyrimidine Dimers-
dc.subject.meshUltraviolet Rays-
dc.titleHuman melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers.en
dc.typeArticleen
dc.contributor.departmentDepartment of Photobiology, St John's Institute for Dermatology, St Thomas Hospital, London, UK.en
dc.identifier.journalThe Journal of Investigative Dermatologyen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.