Structural comparison of fibroblast growth factor-specific heparan sulfates derived from a growing or differentiating neuroepithelial cell line.

2.50
Hdl Handle:
http://hdl.handle.net/10541/93005
Title:
Structural comparison of fibroblast growth factor-specific heparan sulfates derived from a growing or differentiating neuroepithelial cell line.
Authors:
Brickman, Y G; Nurcombe, V; Ford, M D; Gallagher, John T; Bartlett, P F; Turnbull, Jeremy E
Abstract:
Heparan sulfate (HS) glycosaminoglycans are essential modulators of fibroblast growth factor (FGF) activity both in vivo and in vitro, and appear to act by cross-linking particular forms of FGF to appropriate FGF receptors. We have recently isolated and characterized two separate HS pools derived from immortalized embryonic day 10 mouse neuroepithelial 2.3D cells: one from cells in log growth phase, which greatly potentiates the activity of FGF-2, and the other from cells undergoing contact-inhibition and differentiation, which preferentially activates FGF-1. These two pools of HS have very similar functional activities to those species isolated from primary neuroepithelial cells at corresponding stages of active proliferation or differentiation. We present here a structural comparison between these cell line HS species to establish the nature of the changes that occur in the biosynthesis of HS. A combination of chemical and enzymatic cleavage, low pressure chromatography and strong anion-exchange HPLC were used to generate full chain models of each species. Overall, the HS pools synthesized in the dividing cell line pools possessed less complex sulfation than those derived from more differentiated, growth arrested cells.
Affiliation:
Department of Anatomy and Cell Biology, University of Melbourne, Victoria, Australia.
Citation:
Structural comparison of fibroblast growth factor-specific heparan sulfates derived from a growing or differentiating neuroepithelial cell line. 1998, 8 (5):463-71 Glycobiology
Journal:
Glycobiology
Issue Date:
May-1998
URI:
http://hdl.handle.net/10541/93005
PubMed ID:
9597544
Type:
Article
Language:
en
ISSN:
0959-6658
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBrickman, Y Gen
dc.contributor.authorNurcombe, Ven
dc.contributor.authorFord, M Den
dc.contributor.authorGallagher, John Ten
dc.contributor.authorBartlett, P Fen
dc.contributor.authorTurnbull, Jeremy Een
dc.date.accessioned2010-02-25T11:50:01Z-
dc.date.available2010-02-25T11:50:01Z-
dc.date.issued1998-05-
dc.identifier.citationStructural comparison of fibroblast growth factor-specific heparan sulfates derived from a growing or differentiating neuroepithelial cell line. 1998, 8 (5):463-71 Glycobiologyen
dc.identifier.issn0959-6658-
dc.identifier.pmid9597544-
dc.identifier.urihttp://hdl.handle.net/10541/93005-
dc.description.abstractHeparan sulfate (HS) glycosaminoglycans are essential modulators of fibroblast growth factor (FGF) activity both in vivo and in vitro, and appear to act by cross-linking particular forms of FGF to appropriate FGF receptors. We have recently isolated and characterized two separate HS pools derived from immortalized embryonic day 10 mouse neuroepithelial 2.3D cells: one from cells in log growth phase, which greatly potentiates the activity of FGF-2, and the other from cells undergoing contact-inhibition and differentiation, which preferentially activates FGF-1. These two pools of HS have very similar functional activities to those species isolated from primary neuroepithelial cells at corresponding stages of active proliferation or differentiation. We present here a structural comparison between these cell line HS species to establish the nature of the changes that occur in the biosynthesis of HS. A combination of chemical and enzymatic cleavage, low pressure chromatography and strong anion-exchange HPLC were used to generate full chain models of each species. Overall, the HS pools synthesized in the dividing cell line pools possessed less complex sulfation than those derived from more differentiated, growth arrested cells.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCarbohydrate Sequence-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshCell Line-
dc.subject.meshChromatography, Gel-
dc.subject.meshChromatography, Ion Exchange-
dc.subject.meshDisaccharides-
dc.subject.meshDrug Synergism-
dc.subject.meshEmbryo, Mammalian-
dc.subject.meshEpithelial Cells-
dc.subject.meshFibroblast Growth Factor 1-
dc.subject.meshFibroblast Growth Factor 2-
dc.subject.meshHeparin Lyase-
dc.subject.meshHeparitin Sulfate-
dc.subject.meshMice-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshNitrous Acid-
dc.subject.meshOligosaccharides-
dc.titleStructural comparison of fibroblast growth factor-specific heparan sulfates derived from a growing or differentiating neuroepithelial cell line.en
dc.typeArticleen
dc.contributor.departmentDepartment of Anatomy and Cell Biology, University of Melbourne, Victoria, Australia.en
dc.identifier.journalGlycobiologyen

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