CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/93003
Title:
CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
Authors:
De Wynter, Erika A; Buck, D; Hart, Claire A; Heywood, R; Coutinho, Lucia H; Clayton, Alison J; Rafferty, Joseph A; Burt, Deborah J; Guenechea, G; Bueren, J A; Gagen, D; Fairbairn, Leslie J; Lord, Brian I; Testa, Nydia G
Abstract:
The AC133 antigen is a novel antigen selectively expressed on a subset of CD34+ cells in human fetal liver, bone marrow, and blood as demonstrated by flow cytometric analyses. In this study, we have further assessed the expression of AC133 on CD34+ cells in hemopoietic samples and found that there was a highly significant difference between normal bone marrow and cord blood versus aphereses (p <0.0001) but not between bone marrow and cord blood. Most of the clonogenic cells (67%) were contained in the CD34+AC133+ fraction. Compared with cultures of the CD34+AC133- cells, generation of progenitor cells in long-term culture on bone marrow stroma was consistently 10- to 100-fold higher in cultures initiated with CD34+AC133+ cells and was maintained for the 8-10 weeks of culture. Only the CD34+AC133+ cells were capable of repopulating NOD/SCID mice. Human cells were detectable as early as day 20, with increased levels (67%) apparent 40 days post-transplantation. Five thousand CD34+AC133+ cells engrafted about 20% of the mice, while no engraftment was observed in animals transplanted with up to 1.2 x 10(5) CD34+AC133- cells. The CD34+AC133+ population was also enriched (seven-fold) in dendritic cell precursors, and the dendritic cells generated were functionally active in a mixed lymphocyte reaction assay. AC133+ cells should be useful in the study of cellular and molecular mechanisms regulating primitive hemopoietic cells.
Affiliation:
CRC Section of Haemopoietic Cell and Gene Therapeutics, Paterson Institute for Cancer Research, Withington, Manchester, United Kingdom.
Citation:
CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors. 1998, 16 (6):387-96 Stem Cells
Journal:
Stem Cells
Issue Date:
1998
URI:
http://hdl.handle.net/10541/93003
DOI:
10.1002/stem.160387
PubMed ID:
9831864
Type:
Article
Language:
en
ISSN:
1066-5099
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorDe Wynter, Erika Aen
dc.contributor.authorBuck, Den
dc.contributor.authorHart, Claire Aen
dc.contributor.authorHeywood, Ren
dc.contributor.authorCoutinho, Lucia Hen
dc.contributor.authorClayton, Alison Jen
dc.contributor.authorRafferty, Joseph Aen
dc.contributor.authorBurt, Deborah Jen
dc.contributor.authorGuenechea, Gen
dc.contributor.authorBueren, J Aen
dc.contributor.authorGagen, Den
dc.contributor.authorFairbairn, Leslie Jen
dc.contributor.authorLord, Brian Ien
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-02-25T11:38:03Z-
dc.date.available2010-02-25T11:38:03Z-
dc.date.issued1998-
dc.identifier.citationCD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors. 1998, 16 (6):387-96 Stem Cellsen
dc.identifier.issn1066-5099-
dc.identifier.pmid9831864-
dc.identifier.doi10.1002/stem.160387-
dc.identifier.urihttp://hdl.handle.net/10541/93003-
dc.description.abstractThe AC133 antigen is a novel antigen selectively expressed on a subset of CD34+ cells in human fetal liver, bone marrow, and blood as demonstrated by flow cytometric analyses. In this study, we have further assessed the expression of AC133 on CD34+ cells in hemopoietic samples and found that there was a highly significant difference between normal bone marrow and cord blood versus aphereses (p <0.0001) but not between bone marrow and cord blood. Most of the clonogenic cells (67%) were contained in the CD34+AC133+ fraction. Compared with cultures of the CD34+AC133- cells, generation of progenitor cells in long-term culture on bone marrow stroma was consistently 10- to 100-fold higher in cultures initiated with CD34+AC133+ cells and was maintained for the 8-10 weeks of culture. Only the CD34+AC133+ cells were capable of repopulating NOD/SCID mice. Human cells were detectable as early as day 20, with increased levels (67%) apparent 40 days post-transplantation. Five thousand CD34+AC133+ cells engrafted about 20% of the mice, while no engraftment was observed in animals transplanted with up to 1.2 x 10(5) CD34+AC133- cells. The CD34+AC133+ population was also enriched (seven-fold) in dendritic cell precursors, and the dendritic cells generated were functionally active in a mixed lymphocyte reaction assay. AC133+ cells should be useful in the study of cellular and molecular mechanisms regulating primitive hemopoietic cells.en
dc.language.isoenen
dc.subjectFoetal Blooden
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshAntigens, CD-
dc.subject.meshAntigens, CD34-
dc.subject.meshCell Culture Techniques-
dc.subject.meshDendritic Cells-
dc.subject.meshFetal Blood-
dc.subject.meshFlow Cytometry-
dc.subject.meshGlycoproteins-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshMice-
dc.subject.meshMice, Inbred NOD-
dc.subject.meshMice, SCID-
dc.subject.meshPeptides-
dc.subject.meshTime Factors-
dc.titleCD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.en
dc.typeArticleen
dc.contributor.departmentCRC Section of Haemopoietic Cell and Gene Therapeutics, Paterson Institute for Cancer Research, Withington, Manchester, United Kingdom.en
dc.identifier.journalStem Cellsen

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