Induction of lympho-haemopoietic malignancy: impact of preconception paternal irradiation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92934
Title:
Induction of lympho-haemopoietic malignancy: impact of preconception paternal irradiation.
Authors:
Lord, Brian I; Woolford, Lorna B; Wang, L; McDonald, D; Lorimore, S A; Stones, V A; Wright, Eric G; Scott, David
Abstract:
PURPOSE: To investigate the effects of preconception paternal irradiation (PPI) from injected 239Pu on the susceptibility to induction of lympho-haemopoietic malignancy by subsequent irradiation or exposure to a chemical carcinogen. MATERIALS AND METHODS: The male CBA/H and DBA2 mouse was injected with 0, 128 or 256 Bqg(-1) 239Pu 12 weeks before mating with the normal CBA/H and C57B1 female respectively. CBA/H offspring were exposed to 3.3 Gy gamma-rays total body irradiation: BDF1 offspring were injected with 50 mg kg(-1) methyl nitrosourea (MNU). The offspring were assayed for changes in bone marrow progenitor cell numbers and chromosome aberrations and were followed up for subsequent induction of neoplasia. RESULTS: While the untreated mouse showed a normal distribution for cellularity, spleen colony-forming units (CFU-S) and fibroblastoid colony-forming units (CFU-F), significant numbers of PPI offspring presented levels outside the normal range. There was a tendency for them also to show increased, dose-related, levels of chromosomal aberrations. Offspring treated with irradiation or MNU developed an increased incidence of lympho-haemopoietic malignancies. CONCLUSIONS: These studies have shown that PPI results in offspring that are more susceptible to the induction of lymphohaemopoietic malignancy on encountering a secondary carcinogenic insult. This may be linked to inherited chromosomal instability and abnormal kinetics of haemopoiesis. The experiments indicate a potential mechanism by which an increased incidence of leukaemia may be linked to PPI.
Affiliation:
CRC Sections of Experimental Haematology and Molecular Genetics, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Induction of lympho-haemopoietic malignancy: impact of preconception paternal irradiation. 1998, 74 (6):721-8 Int. J. Radiat. Biol.
Journal:
International Journal of Radiation Biology
Issue Date:
Dec-1998
URI:
http://hdl.handle.net/10541/92934
PubMed ID:
9881717
Type:
Article
Language:
en
ISSN:
0955-3002
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLord, Brian Ien
dc.contributor.authorWoolford, Lorna Ben
dc.contributor.authorWang, Len
dc.contributor.authorMcDonald, Den
dc.contributor.authorLorimore, S Aen
dc.contributor.authorStones, V Aen
dc.contributor.authorWright, Eric Gen
dc.contributor.authorScott, Daviden
dc.date.accessioned2010-02-24T14:06:30Z-
dc.date.available2010-02-24T14:06:30Z-
dc.date.issued1998-12-
dc.identifier.citationInduction of lympho-haemopoietic malignancy: impact of preconception paternal irradiation. 1998, 74 (6):721-8 Int. J. Radiat. Biol.en
dc.identifier.issn0955-3002-
dc.identifier.pmid9881717-
dc.identifier.urihttp://hdl.handle.net/10541/92934-
dc.description.abstractPURPOSE: To investigate the effects of preconception paternal irradiation (PPI) from injected 239Pu on the susceptibility to induction of lympho-haemopoietic malignancy by subsequent irradiation or exposure to a chemical carcinogen. MATERIALS AND METHODS: The male CBA/H and DBA2 mouse was injected with 0, 128 or 256 Bqg(-1) 239Pu 12 weeks before mating with the normal CBA/H and C57B1 female respectively. CBA/H offspring were exposed to 3.3 Gy gamma-rays total body irradiation: BDF1 offspring were injected with 50 mg kg(-1) methyl nitrosourea (MNU). The offspring were assayed for changes in bone marrow progenitor cell numbers and chromosome aberrations and were followed up for subsequent induction of neoplasia. RESULTS: While the untreated mouse showed a normal distribution for cellularity, spleen colony-forming units (CFU-S) and fibroblastoid colony-forming units (CFU-F), significant numbers of PPI offspring presented levels outside the normal range. There was a tendency for them also to show increased, dose-related, levels of chromosomal aberrations. Offspring treated with irradiation or MNU developed an increased incidence of lympho-haemopoietic malignancies. CONCLUSIONS: These studies have shown that PPI results in offspring that are more susceptible to the induction of lymphohaemopoietic malignancy on encountering a secondary carcinogenic insult. This may be linked to inherited chromosomal instability and abnormal kinetics of haemopoiesis. The experiments indicate a potential mechanism by which an increased incidence of leukaemia may be linked to PPI.en
dc.language.isoenen
dc.subjectExperimental Leukaemiaen
dc.subjectRadiation-Induced Canceren
dc.subject.meshAnimals-
dc.subject.meshCarcinogens-
dc.subject.meshCells, Cultured-
dc.subject.meshChromosome Aberrations-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshFemale-
dc.subject.meshGamma Rays-
dc.subject.meshLeukemia, Experimental-
dc.subject.meshMale-
dc.subject.meshMethylnitrosourea-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshMutagens-
dc.subject.meshNeoplasms, Radiation-Induced-
dc.subject.meshPaternal Exposure-
dc.subject.meshPlutonium-
dc.subject.meshPregnancy-
dc.subject.meshPrenatal Exposure Delayed Effects-
dc.subject.meshSpleen-
dc.subject.meshWhole-Body Irradiation-
dc.titleInduction of lympho-haemopoietic malignancy: impact of preconception paternal irradiation.en
dc.typeArticleen
dc.contributor.departmentCRC Sections of Experimental Haematology and Molecular Genetics, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalInternational Journal of Radiation Biologyen
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