Repair of radiation-induced DNA double-strand breaks in human fibroblasts is consistent with a continuous spectrum of repair probability.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92926
Title:
Repair of radiation-induced DNA double-strand breaks in human fibroblasts is consistent with a continuous spectrum of repair probability.
Authors:
Foray, N; Monroco, C; Marples, Brian; Hendry, Jolyon H; Fertil, B; Goodhead, D T; Arlett, C F; Malaise, E P
Abstract:
PURPOSE: To propose a novel interpretation of DNA double-strand break (dsb) repair based on the distribution of energy micro-deposition. MATERIALS AND METHODS: Double-strand break repair curves were studied either after irradiation at 4 degrees C or at 37 degrees C (low dose rate). Two human fibroblast cell lines were used: a control line, HF19, and an ataxia telangiectasia repair-deficient line, AT5BI. Irradiations were made with gamma-rays or alpha-particles (241Am). Repair data were fitted by the variable repair half-time (VRHT) model. Assuming that each dsb has its own inherent repair half-time (IRHT) and that the VRHT is the average of the IRHT at any time during repair, the distribution of the IRHT was calculated. RESULTS: At the end of the irradiation, the distribution was a continuous asymmetric curve with a maximum of dsb having a short IRHT. After 1 h of repair, the curve became bell-shaped. There is a striking similarity between the distribution of dsb repair half-times and that of energy micro-deposition described by Goodhead et al. (1993). CONCLUSION: This similarity suggests a possible causal relationship between the energy density deposition and the repair rate or the probability of dsb repair.
Affiliation:
UMR 1599 CNRS - PR1 - Institut Gustave-Roussy, Villejuif, France. nforay@igr.fr
Citation:
Repair of radiation-induced DNA double-strand breaks in human fibroblasts is consistent with a continuous spectrum of repair probability. 1998, 74 (5):551-60 Int. J. Radiat. Biol.
Journal:
International Journal of Radiation Biology
Issue Date:
Nov-1998
URI:
http://hdl.handle.net/10541/92926
PubMed ID:
9848273
Type:
Article
Language:
en
ISSN:
0955-3002
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorForay, Nen
dc.contributor.authorMonroco, Cen
dc.contributor.authorMarples, Brianen
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorFertil, Ben
dc.contributor.authorGoodhead, D Ten
dc.contributor.authorArlett, C Fen
dc.contributor.authorMalaise, E Pen
dc.date.accessioned2010-02-24T13:59:43Z-
dc.date.available2010-02-24T13:59:43Z-
dc.date.issued1998-11-
dc.identifier.citationRepair of radiation-induced DNA double-strand breaks in human fibroblasts is consistent with a continuous spectrum of repair probability. 1998, 74 (5):551-60 Int. J. Radiat. Biol.en
dc.identifier.issn0955-3002-
dc.identifier.pmid9848273-
dc.identifier.urihttp://hdl.handle.net/10541/92926-
dc.description.abstractPURPOSE: To propose a novel interpretation of DNA double-strand break (dsb) repair based on the distribution of energy micro-deposition. MATERIALS AND METHODS: Double-strand break repair curves were studied either after irradiation at 4 degrees C or at 37 degrees C (low dose rate). Two human fibroblast cell lines were used: a control line, HF19, and an ataxia telangiectasia repair-deficient line, AT5BI. Irradiations were made with gamma-rays or alpha-particles (241Am). Repair data were fitted by the variable repair half-time (VRHT) model. Assuming that each dsb has its own inherent repair half-time (IRHT) and that the VRHT is the average of the IRHT at any time during repair, the distribution of the IRHT was calculated. RESULTS: At the end of the irradiation, the distribution was a continuous asymmetric curve with a maximum of dsb having a short IRHT. After 1 h of repair, the curve became bell-shaped. There is a striking similarity between the distribution of dsb repair half-times and that of energy micro-deposition described by Goodhead et al. (1993). CONCLUSION: This similarity suggests a possible causal relationship between the energy density deposition and the repair rate or the probability of dsb repair.en
dc.language.isoenen
dc.subject.meshAmericium-
dc.subject.meshCell Line-
dc.subject.meshDNA-
dc.subject.meshDNA Repair-
dc.subject.meshFibroblasts-
dc.subject.meshHumans-
dc.subject.meshTemperature-
dc.subject.meshTime Factors-
dc.titleRepair of radiation-induced DNA double-strand breaks in human fibroblasts is consistent with a continuous spectrum of repair probability.en
dc.typeArticleen
dc.contributor.departmentUMR 1599 CNRS - PR1 - Institut Gustave-Roussy, Villejuif, France. nforay@igr.fren
dc.identifier.journalInternational Journal of Radiation Biologyen
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