Altered stem cell regeneration in irradiated intestinal crypts of senescent mice.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92918
Title:
Altered stem cell regeneration in irradiated intestinal crypts of senescent mice.
Authors:
Martin, Kareen; Potten, Christopher S; Roberts, Stephen A; Kirkwood, T B
Abstract:
Ageing is associated with a progressive deterioration in the functions of many organs within the body. In tissue with high cell turnover, the maintenance of the stem cells is of particular importance. Any accumulation of damage in stem cells may affect their function and hence threaten the homeostasis and regenerative capacity of the tissue. The small intestine represents a good model for the study of stem cells because of its spatial and hierarchical organisation. We have examined the effect of age on stem cell regenerative capacity after irradiation, using the microcolony assay. Crypt survival levels, the growth rate of surviving crypts, and the number of cells able to repopulate a crypt have been investigated by irradiating groups of 6-7 month old and 28-30 month old ICRFa male mice. After high doses of irradiation, the surviving crypts in old mice were both smaller and fewer in number than in young mice. The growth rate of surviving crypts was determined by measuring the crypt area and the number of cells/crypt at various times after 14 Gy irradiation. There was a growth delay of between about one half and one day in the older mice. Surprisingly, the number of clonogenic cells per crypt was estimated to be greater in the older mice. These studies indicate important age-related alterations in the capacity to regenerate the crypts after radiation damage.
Affiliation:
Biological Gerontology Group, University of Manchester, Oxford Road, Manchester M13 9PT, UK.
Citation:
Altered stem cell regeneration in irradiated intestinal crypts of senescent mice. 1998, 111 ( Pt 16):2297-303 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
Aug-1998
URI:
http://hdl.handle.net/10541/92918
PubMed ID:
9683625
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMartin, Kareenen
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorRoberts, Stephen Aen
dc.contributor.authorKirkwood, T Ben
dc.date.accessioned2010-02-24T13:21:25Z-
dc.date.available2010-02-24T13:21:25Z-
dc.date.issued1998-08-
dc.identifier.citationAltered stem cell regeneration in irradiated intestinal crypts of senescent mice. 1998, 111 ( Pt 16):2297-303 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid9683625-
dc.identifier.urihttp://hdl.handle.net/10541/92918-
dc.description.abstractAgeing is associated with a progressive deterioration in the functions of many organs within the body. In tissue with high cell turnover, the maintenance of the stem cells is of particular importance. Any accumulation of damage in stem cells may affect their function and hence threaten the homeostasis and regenerative capacity of the tissue. The small intestine represents a good model for the study of stem cells because of its spatial and hierarchical organisation. We have examined the effect of age on stem cell regenerative capacity after irradiation, using the microcolony assay. Crypt survival levels, the growth rate of surviving crypts, and the number of cells able to repopulate a crypt have been investigated by irradiating groups of 6-7 month old and 28-30 month old ICRFa male mice. After high doses of irradiation, the surviving crypts in old mice were both smaller and fewer in number than in young mice. The growth rate of surviving crypts was determined by measuring the crypt area and the number of cells/crypt at various times after 14 Gy irradiation. There was a growth delay of between about one half and one day in the older mice. Surprisingly, the number of clonogenic cells per crypt was estimated to be greater in the older mice. These studies indicate important age-related alterations in the capacity to regenerate the crypts after radiation damage.en
dc.language.isoenen
dc.subject.meshAging-
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshCell Division-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshIleum-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshRadiation Tolerance-
dc.subject.meshRegeneration-
dc.subject.meshStem Cells-
dc.titleAltered stem cell regeneration in irradiated intestinal crypts of senescent mice.en
dc.typeArticleen
dc.contributor.departmentBiological Gerontology Group, University of Manchester, Oxford Road, Manchester M13 9PT, UK.en
dc.identifier.journalJournal of Cell Scienceen
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