Frequent alterations of cell cycle regulators in early-stage breast lesions as detected by immunohistochemistry.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92917
Title:
Frequent alterations of cell cycle regulators in early-stage breast lesions as detected by immunohistochemistry.
Authors:
Marsh, K L; Varley, Jennifer
Abstract:
Progression through G1 phase of the eukaryotic cell cycle is tightly controlled by cyclin-dependent kinases (CDK). These proteins form part of a regulatory pathway including the cyclin-dependent kinase inhibitor (CKI) p16, D-type cyclins and the product of the retinoblastoma gene pRb. Aberration of any one of these components may lead to uncontrolled proliferation contributing to neoplasia. Three of these proteins, cyclin D1, pRb and p16, were analysed by immunohistochemistry on archival paraffin sections to determine whether expression patterns were different in preinvasive ductal carcinoma in situ (DCIS) and invasive breast tumours relative to normal. Genetic analysis of the gene encoding cyclin D1 (CCND1) was also carried out, using an intragenic restriction fragment-length polymorphism (RFLP) to assess possible allelic imbalance. A majority of the tumours studied (approximately 90%) showed abnormalities in expression of at least one of these proteins. Overexpression of cyclin D1 was found in approximately 49% cases, reduced expression of p16 in approximately 46% and reduced expression of pRb in approximately 37%. Allelic imbalance of cyclin D1 was found in approximately 57% cases.
Affiliation:
CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
Citation:
Frequent alterations of cell cycle regulators in early-stage breast lesions as detected by immunohistochemistry. 1998, 77 (9):1460-8 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
May-1998
URI:
http://hdl.handle.net/10541/92917
PubMed ID:
9652762
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMarsh, K Len
dc.contributor.authorVarley, Jenniferen
dc.date.accessioned2010-02-24T13:18:58Z-
dc.date.available2010-02-24T13:18:58Z-
dc.date.issued1998-05-
dc.identifier.citationFrequent alterations of cell cycle regulators in early-stage breast lesions as detected by immunohistochemistry. 1998, 77 (9):1460-8 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid9652762-
dc.identifier.urihttp://hdl.handle.net/10541/92917-
dc.description.abstractProgression through G1 phase of the eukaryotic cell cycle is tightly controlled by cyclin-dependent kinases (CDK). These proteins form part of a regulatory pathway including the cyclin-dependent kinase inhibitor (CKI) p16, D-type cyclins and the product of the retinoblastoma gene pRb. Aberration of any one of these components may lead to uncontrolled proliferation contributing to neoplasia. Three of these proteins, cyclin D1, pRb and p16, were analysed by immunohistochemistry on archival paraffin sections to determine whether expression patterns were different in preinvasive ductal carcinoma in situ (DCIS) and invasive breast tumours relative to normal. Genetic analysis of the gene encoding cyclin D1 (CCND1) was also carried out, using an intragenic restriction fragment-length polymorphism (RFLP) to assess possible allelic imbalance. A majority of the tumours studied (approximately 90%) showed abnormalities in expression of at least one of these proteins. Overexpression of cyclin D1 was found in approximately 49% cases, reduced expression of p16 in approximately 46% and reduced expression of pRb in approximately 37%. Allelic imbalance of cyclin D1 was found in approximately 57% cases.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectCancer Proteinsen
dc.subject.meshAlleles-
dc.subject.meshBreast Neoplasms-
dc.subject.meshCarcinoma, Ductal, Breast-
dc.subject.meshCyclin D1-
dc.subject.meshCyclin-Dependent Kinase Inhibitor p16-
dc.subject.meshCyclin-Dependent Kinases-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshRetinoblastoma Protein-
dc.titleFrequent alterations of cell cycle regulators in early-stage breast lesions as detected by immunohistochemistry.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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