Expression patterns of the human papillomavirus type 16 transcription factor E2 in low- and high-grade cervical intraepithelial neoplasia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92906
Title:
Expression patterns of the human papillomavirus type 16 transcription factor E2 in low- and high-grade cervical intraepithelial neoplasia.
Authors:
Maitland, N J; Conway, S; Wilkinson, N S; Ramsdale, J; Morris, J R; Sanders, C M; Burns, J E; Stern, Peter L; Wells, M
Abstract:
Specific antibodies against the C-terminus of E2, produced by affinity purification of polyclonal antisera, have been used to identify the cellular populations which express the HPV 16 E2 transcription factor, in a series of formalin-fixed, paraffin-embedded cervical tissues. Cases were selected for both the presence of HPV 16 DNA (confirmed by multiple gene-specific PCR detections) and the presence of multiple grades of cervical intraepithelial neoplasia (CIN). The data indicate that E2 expression is highest in CIN I and in koilocytic lesions. Lower expression was observed in CIN II and little in CIN III lesions. In contrast, there was some restoration of E2 expression in invasive carcinomas, although the intracellular distribution was much more diffuse. The location of E2 expression to the superficial layers of the cervical epithelium, as well as the occurrence of some basal expression in CIN I, suggests that antibodies against HPV 16 E2 could be a useful adjunct to standard histological techniques for the detection of 'at-risk' patients as part of a cervical screening programme.
Affiliation:
Department of Biology, University of York, U.K. njm9@york.ac.uk
Citation:
Expression patterns of the human papillomavirus type 16 transcription factor E2 in low- and high-grade cervical intraepithelial neoplasia. 1998, 186 (3):275-80 J. Pathol.
Journal:
The Journal of Pathology
Issue Date:
Nov-1998
URI:
http://hdl.handle.net/10541/92906
DOI:
10.1002/(SICI)1096-9896(1998110)186:3<275::AID-PATH159>3.0.CO;2-E
PubMed ID:
10211116
Type:
Article
Language:
en
ISSN:
0022-3417
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMaitland, N Jen
dc.contributor.authorConway, Sen
dc.contributor.authorWilkinson, N Sen
dc.contributor.authorRamsdale, Jen
dc.contributor.authorMorris, J Ren
dc.contributor.authorSanders, C Men
dc.contributor.authorBurns, J Een
dc.contributor.authorStern, Peter Len
dc.contributor.authorWells, Men
dc.date.accessioned2010-02-24T13:57:47Z-
dc.date.available2010-02-24T13:57:47Z-
dc.date.issued1998-11-
dc.identifier.citationExpression patterns of the human papillomavirus type 16 transcription factor E2 in low- and high-grade cervical intraepithelial neoplasia. 1998, 186 (3):275-80 J. Pathol.en
dc.identifier.issn0022-3417-
dc.identifier.pmid10211116-
dc.identifier.doi10.1002/(SICI)1096-9896(1998110)186:3<275::AID-PATH159>3.0.CO;2-E-
dc.identifier.urihttp://hdl.handle.net/10541/92906-
dc.description.abstractSpecific antibodies against the C-terminus of E2, produced by affinity purification of polyclonal antisera, have been used to identify the cellular populations which express the HPV 16 E2 transcription factor, in a series of formalin-fixed, paraffin-embedded cervical tissues. Cases were selected for both the presence of HPV 16 DNA (confirmed by multiple gene-specific PCR detections) and the presence of multiple grades of cervical intraepithelial neoplasia (CIN). The data indicate that E2 expression is highest in CIN I and in koilocytic lesions. Lower expression was observed in CIN II and little in CIN III lesions. In contrast, there was some restoration of E2 expression in invasive carcinomas, although the intracellular distribution was much more diffuse. The location of E2 expression to the superficial layers of the cervical epithelium, as well as the occurrence of some basal expression in CIN I, suggests that antibodies against HPV 16 E2 could be a useful adjunct to standard histological techniques for the detection of 'at-risk' patients as part of a cervical screening programme.en
dc.language.isoenen
dc.subjectUterine Cervical Canceren
dc.subject.meshCervical Intraepithelial Neoplasia-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshOncogene Proteins, Viral-
dc.subject.meshPapillomaviridae-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleExpression patterns of the human papillomavirus type 16 transcription factor E2 in low- and high-grade cervical intraepithelial neoplasia.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biology, University of York, U.K. njm9@york.ac.uken
dc.identifier.journalThe Journal of Pathologyen

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