Regulation of the proliferative potential of cord blood long-term culture-initiating cells (LTC-IC) by different stromal cell lines: implications for LTC-IC measurement.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92900
Title:
Regulation of the proliferative potential of cord blood long-term culture-initiating cells (LTC-IC) by different stromal cell lines: implications for LTC-IC measurement.
Authors:
Nadali, Gianpaolo; De Wynter, Erika A; Perandin, F; Tavecchia, L; Vincenzi, C; Ambrosetti, A; Fornalè, M; Perona, Giuseppe; Pizzolo, Giovanni; Testa, Nydia G
Abstract:
BACKGROUND AND OBJECTIVE: Long-term culture-initiating cells (LTC-IC) are the best available approximation to an in vitro assay of stem cells in humans although they still represent a heterogeneous population in terms of proliferative capacity and sensitivity to different growth factors. Human umbilical cord blood (CB) is rich in hemopoietic progenitor cells, as measured by clonogenic assays and contains stem cells capable of reconstituting the marrow after ablation in clinical transplantation. We evaluated the influence of culture conditions on the in vitro behavior of LTC-IC from CB. DESIGN AND METHODS: LTC-IC were evaluated in long-term cultures, comparing two types of murine stromal cell lines: M2-10B4 and M2-10B4 transfected with cDNAs for human G-CSF and IL-3. RESULTS: Two and five fold higher numbers of terminally differentiated cells were produced during nine weeks of culture of CB mononuclear or CD34+ cells respectively, in cultures containing a M2-10B4 IL-3 G-CSF cell line compared to cultures containing the parental cell line. Likewise, a higher number of colony-forming cells (CFC) were detected in the supernatant of cultures with the transfected cell line. In contrast, the number of CFC generated within the stromal layer, after 5 or 9 weeks of culture, was significantly higher in cultures on M2-10B4 cells than those on M2-10B4 IL-3 G-CSF. INTERPRETATION AND CONCLUSIONS: Our results show that the proliferative capacity of CB LTC-IC can be strongly influenced by culture conditions and that the frequency of LTC-IC estimated using these cell lines as stromal support is not identical.
Affiliation:
Department of Hematology, University of Verona, Italy. gnadali@borgoroma.univr.it
Citation:
Regulation of the proliferative potential of cord blood long-term culture-initiating cells (LTC-IC) by different stromal cell lines: implications for LTC-IC measurement. 1998, 83 (12):1059-65 Haematologica
Journal:
Haematologica
Issue Date:
Dec-1998
URI:
http://hdl.handle.net/10541/92900
PubMed ID:
9949621
Type:
Article
Language:
en
ISSN:
0390-6078
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorNadali, Gianpaoloen
dc.contributor.authorDe Wynter, Erika Aen
dc.contributor.authorPerandin, Fen
dc.contributor.authorTavecchia, Len
dc.contributor.authorVincenzi, Cen
dc.contributor.authorAmbrosetti, Aen
dc.contributor.authorFornalè, Men
dc.contributor.authorPerona, Giuseppeen
dc.contributor.authorPizzolo, Giovannien
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-02-24T12:48:18Z-
dc.date.available2010-02-24T12:48:18Z-
dc.date.issued1998-12-
dc.identifier.citationRegulation of the proliferative potential of cord blood long-term culture-initiating cells (LTC-IC) by different stromal cell lines: implications for LTC-IC measurement. 1998, 83 (12):1059-65 Haematologicaen
dc.identifier.issn0390-6078-
dc.identifier.pmid9949621-
dc.identifier.urihttp://hdl.handle.net/10541/92900-
dc.description.abstractBACKGROUND AND OBJECTIVE: Long-term culture-initiating cells (LTC-IC) are the best available approximation to an in vitro assay of stem cells in humans although they still represent a heterogeneous population in terms of proliferative capacity and sensitivity to different growth factors. Human umbilical cord blood (CB) is rich in hemopoietic progenitor cells, as measured by clonogenic assays and contains stem cells capable of reconstituting the marrow after ablation in clinical transplantation. We evaluated the influence of culture conditions on the in vitro behavior of LTC-IC from CB. DESIGN AND METHODS: LTC-IC were evaluated in long-term cultures, comparing two types of murine stromal cell lines: M2-10B4 and M2-10B4 transfected with cDNAs for human G-CSF and IL-3. RESULTS: Two and five fold higher numbers of terminally differentiated cells were produced during nine weeks of culture of CB mononuclear or CD34+ cells respectively, in cultures containing a M2-10B4 IL-3 G-CSF cell line compared to cultures containing the parental cell line. Likewise, a higher number of colony-forming cells (CFC) were detected in the supernatant of cultures with the transfected cell line. In contrast, the number of CFC generated within the stromal layer, after 5 or 9 weeks of culture, was significantly higher in cultures on M2-10B4 cells than those on M2-10B4 IL-3 G-CSF. INTERPRETATION AND CONCLUSIONS: Our results show that the proliferative capacity of CB LTC-IC can be strongly influenced by culture conditions and that the frequency of LTC-IC estimated using these cell lines as stromal support is not identical.en
dc.language.isoenen
dc.subjectFoetal Blooden
dc.subjectHaematopoietic Stem Cell Mobilisationen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshCell Communication-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshCoculture Techniques-
dc.subject.meshFemale-
dc.subject.meshFetal Blood-
dc.subject.meshHematopoietic Stem Cell Mobilization-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshPregnancy-
dc.subject.meshStromal Cells-
dc.subject.meshTime Factors-
dc.titleRegulation of the proliferative potential of cord blood long-term culture-initiating cells (LTC-IC) by different stromal cell lines: implications for LTC-IC measurement.en
dc.typeArticleen
dc.contributor.departmentDepartment of Hematology, University of Verona, Italy. gnadali@borgoroma.univr.iten
dc.identifier.journalHaematologicaen
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