Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92889
Title:
Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line.
Authors:
Pierce, A; Whetton, Anthony D; Owen-Lynch, P J; Tavernier, J; Spooncer, Elaine; Dexter, T Michael; Heyworth, Clare M
Abstract:
The interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific alpha subunit and a common ssc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor alpha subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this alpha subunit. The ectopic expression of the IL-5 receptor alpha subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor alpha subunit.
Affiliation:
Leukemia Research Fund, Cellular Development Unit, UMIST, Manchester M60 1QD, UK.
Citation:
Ectopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line. 1998, 111 ( Pt 6):815-23 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
Mar-1998
URI:
http://hdl.handle.net/10541/92889
PubMed ID:
9472009
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPierce, Aen
dc.contributor.authorWhetton, Anthony Den
dc.contributor.authorOwen-Lynch, P Jen
dc.contributor.authorTavernier, Jen
dc.contributor.authorSpooncer, Elaineen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorHeyworth, Clare Men
dc.date.accessioned2010-02-24T12:09:33Z-
dc.date.available2010-02-24T12:09:33Z-
dc.date.issued1998-03-
dc.identifier.citationEctopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line. 1998, 111 ( Pt 6):815-23 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid9472009-
dc.identifier.urihttp://hdl.handle.net/10541/92889-
dc.description.abstractThe interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific alpha subunit and a common ssc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor alpha subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this alpha subunit. The ectopic expression of the IL-5 receptor alpha subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor alpha subunit.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshCell Line-
dc.subject.meshGene Expression Regulation-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInterleukin-5-
dc.subject.meshMice-
dc.subject.meshReceptors, Interleukin-
dc.subject.meshReceptors, Interleukin-5-
dc.subject.meshTransfection-
dc.titleEctopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line.en
dc.typeArticleen
dc.contributor.departmentLeukemia Research Fund, Cellular Development Unit, UMIST, Manchester M60 1QD, UK.en
dc.identifier.journalJournal of Cell Scienceen
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