Interaction of heparan sulfate from mammary cells with acidic fibroblast growth factor (FGF) and basic FGF. Regulation of the activity of basic FGF by high and low affinity binding sites in heparan sulfate.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92753
Title:
Interaction of heparan sulfate from mammary cells with acidic fibroblast growth factor (FGF) and basic FGF. Regulation of the activity of basic FGF by high and low affinity binding sites in heparan sulfate.
Authors:
Rahmoune, H; Chen, H L; Gallagher, John T; Rudland, P S; Fernig, D G
Abstract:
We have determined the relationship between the binding sites for acidic fibroblast growth factor (aFGF) and basic FGF (bFGF) in heparan sulfate (HS) prepared from a panel of mammary cell lines and the ability of the HS to activate aFGF and bFGF in DNA synthesis assays. The ka of the HS for aFGF fell into three groups, whereas the kd (0.0015-0.016 s-1) and the Kd (0.4-8.6 microM) formed a continuum. bFGF possessed a high affinity binding site (Kd 22-30 nM) with a fast ka (320,000-550,000 M-1 s-1), termed "fast/high," and a lower affinity site (Kd 47-320 nM) with a slower ka (35,000-150,000 M-1 s-1), termed "slow/low." Most of the species of HS possessed the latter binding site, which was able to activate bFGF in HS-deficient fibroblasts. However, the HS from the culture medium of the mammary fibroblasts and the myoepithelial-like cells possessed both a fast/high and a slow/low binding site and could not activate bFGF, although it could potentiate the growth-stimulatory activity of aFGF. Treatment of the HS possessing two binding sites for bFGF with heparitinase 1 released oligosaccharides that were able to restore the activity of bFGF in HS-deficient fibroblasts.
Affiliation:
School of Biological Sciences, Life Sciences Building, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom.
Citation:
Interaction of heparan sulfate from mammary cells with acidic fibroblast growth factor (FGF) and basic FGF. Regulation of the activity of basic FGF by high and low affinity binding sites in heparan sulfate. 1998, 273 (13):7303-10 J. Biol. Chem.
Journal:
The Journal of Biological Chemistry
Issue Date:
27-Mar-1998
URI:
http://hdl.handle.net/10541/92753
PubMed ID:
9516424
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorRahmoune, Hen
dc.contributor.authorChen, H Len
dc.contributor.authorGallagher, John Ten
dc.contributor.authorRudland, P Sen
dc.contributor.authorFernig, D Gen
dc.date.accessioned2010-02-23T13:00:15Z-
dc.date.available2010-02-23T13:00:15Z-
dc.date.issued1998-03-27-
dc.identifier.citationInteraction of heparan sulfate from mammary cells with acidic fibroblast growth factor (FGF) and basic FGF. Regulation of the activity of basic FGF by high and low affinity binding sites in heparan sulfate. 1998, 273 (13):7303-10 J. Biol. Chem.en
dc.identifier.issn0021-9258-
dc.identifier.pmid9516424-
dc.identifier.urihttp://hdl.handle.net/10541/92753-
dc.description.abstractWe have determined the relationship between the binding sites for acidic fibroblast growth factor (aFGF) and basic FGF (bFGF) in heparan sulfate (HS) prepared from a panel of mammary cell lines and the ability of the HS to activate aFGF and bFGF in DNA synthesis assays. The ka of the HS for aFGF fell into three groups, whereas the kd (0.0015-0.016 s-1) and the Kd (0.4-8.6 microM) formed a continuum. bFGF possessed a high affinity binding site (Kd 22-30 nM) with a fast ka (320,000-550,000 M-1 s-1), termed "fast/high," and a lower affinity site (Kd 47-320 nM) with a slower ka (35,000-150,000 M-1 s-1), termed "slow/low." Most of the species of HS possessed the latter binding site, which was able to activate bFGF in HS-deficient fibroblasts. However, the HS from the culture medium of the mammary fibroblasts and the myoepithelial-like cells possessed both a fast/high and a slow/low binding site and could not activate bFGF, although it could potentiate the growth-stimulatory activity of aFGF. Treatment of the HS possessing two binding sites for bFGF with heparitinase 1 released oligosaccharides that were able to restore the activity of bFGF in HS-deficient fibroblasts.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBinding Sites-
dc.subject.meshBreast-
dc.subject.meshCells, Cultured-
dc.subject.meshEpithelial Cells-
dc.subject.meshFemale-
dc.subject.meshFibroblast Growth Factor 1-
dc.subject.meshFibroblast Growth Factor 2-
dc.subject.meshHeparitin Sulfate-
dc.subject.meshHumans-
dc.subject.meshKinetics-
dc.subject.meshMammary Glands, Animal-
dc.subject.meshMolecular Weight-
dc.subject.meshProtein Binding-
dc.subject.meshRats-
dc.subject.meshRecombinant Proteins-
dc.titleInteraction of heparan sulfate from mammary cells with acidic fibroblast growth factor (FGF) and basic FGF. Regulation of the activity of basic FGF by high and low affinity binding sites in heparan sulfate.en
dc.typeArticleen
dc.contributor.departmentSchool of Biological Sciences, Life Sciences Building, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom.en
dc.identifier.journalThe Journal of Biological Chemistryen
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