Assessment of vascularity in histological sections: effects of methodology and value as an index of angiogenesis in breast tumours.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92716
Title:
Assessment of vascularity in histological sections: effects of methodology and value as an index of angiogenesis in breast tumours.
Authors:
Schor, Ana M; Pendleton, N; Pazouki, S; Smither, Rachel L; Morris, J; Lessan, Khashayar; Heerkens, Egidius; Chandrachud, L M; Carmichael, G; Adi, M; Chisholm, D M; Stevenson, H
Abstract:
The aims of this study were to (a) determine how the quantification of blood vessels in histological sections (vascularity) is affected by the methodology used and (b) assess the value of vascularity as an index of angiogenesis by comparing tumour and normal breast tissue. Archival specimens of breast, lung and oral carcinoma, oral dysplasia and normal breast tissue were used to test the effects of the following experimental variables on vascularity: pretreatment of the sections (enzymatic digestion, heating), endothelial markers (von Willebrand factor and CD31 antibodies), method of quantification (highest microvascular density, average microvascular density and microvascular volume) and interobserver variations. All the variables examined significantly affected the estimated vascularity; this depended on the type of tissue and method used. The pretreatment of the sections before staining was the most important variable, altering the vascularity ranking of the tumours. Vascularity in breast tumours was similar to that of the normal breast intralobular stroma, suggesting that an area of high microvascular density in the tumour does not necessarily represent tumour-induced angiogenesis. Contradictory results have been published regarding the value of vascularity as a tumour prognostic factor. Our results suggest that statistically significant differences in vascularity values are most likely to arise from failure to optimize the staining protocol and from the method used to assess vascularity.
Affiliation:
Oral Diseases Group, Dental School, University of Dundee, UK.
Citation:
Assessment of vascularity in histological sections: effects of methodology and value as an index of angiogenesis in breast tumours. 1998, 30 (12):849-56 Histochem. J.
Journal:
The Histochemical Journal
Issue Date:
Dec-1998
URI:
http://hdl.handle.net/10541/92716
PubMed ID:
10100727
Type:
Article
Language:
en
ISSN:
0018-2214
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSchor, Ana Men
dc.contributor.authorPendleton, Nen
dc.contributor.authorPazouki, Sen
dc.contributor.authorSmither, Rachel Len
dc.contributor.authorMorris, Jen
dc.contributor.authorLessan, Khashayaren
dc.contributor.authorHeerkens, Egidiusen
dc.contributor.authorChandrachud, L Men
dc.contributor.authorCarmichael, Gen
dc.contributor.authorAdi, Men
dc.contributor.authorChisholm, D Men
dc.contributor.authorStevenson, Hen
dc.date.accessioned2010-02-23T12:57:10Z-
dc.date.available2010-02-23T12:57:10Z-
dc.date.issued1998-12-
dc.identifier.citationAssessment of vascularity in histological sections: effects of methodology and value as an index of angiogenesis in breast tumours. 1998, 30 (12):849-56 Histochem. J.en
dc.identifier.issn0018-2214-
dc.identifier.pmid10100727-
dc.identifier.urihttp://hdl.handle.net/10541/92716-
dc.description.abstractThe aims of this study were to (a) determine how the quantification of blood vessels in histological sections (vascularity) is affected by the methodology used and (b) assess the value of vascularity as an index of angiogenesis by comparing tumour and normal breast tissue. Archival specimens of breast, lung and oral carcinoma, oral dysplasia and normal breast tissue were used to test the effects of the following experimental variables on vascularity: pretreatment of the sections (enzymatic digestion, heating), endothelial markers (von Willebrand factor and CD31 antibodies), method of quantification (highest microvascular density, average microvascular density and microvascular volume) and interobserver variations. All the variables examined significantly affected the estimated vascularity; this depended on the type of tissue and method used. The pretreatment of the sections before staining was the most important variable, altering the vascularity ranking of the tumours. Vascularity in breast tumours was similar to that of the normal breast intralobular stroma, suggesting that an area of high microvascular density in the tumour does not necessarily represent tumour-induced angiogenesis. Contradictory results have been published regarding the value of vascularity as a tumour prognostic factor. Our results suggest that statistically significant differences in vascularity values are most likely to arise from failure to optimize the staining protocol and from the method used to assess vascularity.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshAntigens, CD31-
dc.subject.meshBlood Vessels-
dc.subject.meshBreast-
dc.subject.meshBreast Neoplasms-
dc.subject.meshCarcinoma, Ductal, Breast-
dc.subject.meshEndopeptidases-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMicrotomy-
dc.subject.meshMicrowaves-
dc.subject.meshMouth Diseases-
dc.subject.meshNeovascularization, Pathologic-
dc.subject.meshObserver Variation-
dc.subject.meshPronase-
dc.subject.meshSeverity of Illness Index-
dc.subject.meshStaining and Labeling-
dc.subject.meshTrypsin-
dc.subject.meshvon Willebrand Factor-
dc.titleAssessment of vascularity in histological sections: effects of methodology and value as an index of angiogenesis in breast tumours.en
dc.typeArticleen
dc.contributor.departmentOral Diseases Group, Dental School, University of Dundee, UK.en
dc.identifier.journalThe Histochemical Journalen

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