Discrimination of human tumor radioresponsiveness using low-dose rate irradiation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92694
Title:
Discrimination of human tumor radioresponsiveness using low-dose rate irradiation.
Authors:
Björk-Eriksson, T; West, Catharine M L; Karlsson, E; Mercke, C
Abstract:
PURPOSE: Evaluation of the theoretical and practical value of using low-dose rate (LDR) irradiation to increase the resolution of radiosensitivity testing of primary human tumors using clonogenic assays. METHODS AND MATERIALS: Fourteen human tumor cell lines were assessed for surviving fraction at 2-8 Gy (SF2-SF8) using low-dose rate irradiation and a clonogenic assay. Further data were collected from the literature for 64 low-dose rate irradiation survival curves from human tumor cell lines. The data were grouped into five different radioresponsiveness categories (A-E). An analysis was made of the ability of the graded survival levels to discriminate between the different radioresponse groups and compared with previous analyses for high-dose rate SF2. Fifteen human cervical carcinoma specimens were analysed for SF2 and SF3.5 following high- and low-dose rate irradiation. RESULTS: Low-dose rate irradiation increased the spread of tumor cell line radiosensitivity data and the ability to discriminate between radioresponse groups was greater at low than at high-dose rates. Using low-dose rate irradiation on primary tumor specimens and a soft agar clonogenic assay decreased the success rate in obtaining data. The latter dropped from 70% for high-dose rate SF2 to 51% for low-dose rate SF3.5. CONCLUSIONS: The work on cell lines illustrates that low-dose rate irradiation does improve the ability of clonogenic radiosensitivity measurements to discriminate between tumors of different radioresponsiveness groups. However, using low-dose rate irradiation on primary human tumors with a soft agar clonogenic assay was not practical because of reducing the success rate for obtaining data for radiosensitivity measurements.
Affiliation:
Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden.
Citation:
Discrimination of human tumor radioresponsiveness using low-dose rate irradiation. 1998, 42 (5):1147-53 Int. J. Radiat. Oncol. Biol. Phys.
Journal:
International Journal of Radiation Oncology, Biology, Physics
Issue Date:
1-Dec-1998
URI:
http://hdl.handle.net/10541/92694
PubMed ID:
9869242
Type:
Article
Language:
en
ISSN:
0360-3016
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBjörk-Eriksson, Ten
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorKarlsson, Een
dc.contributor.authorMercke, Cen
dc.date.accessioned2010-02-23T09:30:20Z-
dc.date.available2010-02-23T09:30:20Z-
dc.date.issued1998-12-01-
dc.identifier.citationDiscrimination of human tumor radioresponsiveness using low-dose rate irradiation. 1998, 42 (5):1147-53 Int. J. Radiat. Oncol. Biol. Phys.en
dc.identifier.issn0360-3016-
dc.identifier.pmid9869242-
dc.identifier.urihttp://hdl.handle.net/10541/92694-
dc.description.abstractPURPOSE: Evaluation of the theoretical and practical value of using low-dose rate (LDR) irradiation to increase the resolution of radiosensitivity testing of primary human tumors using clonogenic assays. METHODS AND MATERIALS: Fourteen human tumor cell lines were assessed for surviving fraction at 2-8 Gy (SF2-SF8) using low-dose rate irradiation and a clonogenic assay. Further data were collected from the literature for 64 low-dose rate irradiation survival curves from human tumor cell lines. The data were grouped into five different radioresponsiveness categories (A-E). An analysis was made of the ability of the graded survival levels to discriminate between the different radioresponse groups and compared with previous analyses for high-dose rate SF2. Fifteen human cervical carcinoma specimens were analysed for SF2 and SF3.5 following high- and low-dose rate irradiation. RESULTS: Low-dose rate irradiation increased the spread of tumor cell line radiosensitivity data and the ability to discriminate between radioresponse groups was greater at low than at high-dose rates. Using low-dose rate irradiation on primary tumor specimens and a soft agar clonogenic assay decreased the success rate in obtaining data. The latter dropped from 70% for high-dose rate SF2 to 51% for low-dose rate SF3.5. CONCLUSIONS: The work on cell lines illustrates that low-dose rate irradiation does improve the ability of clonogenic radiosensitivity measurements to discriminate between tumors of different radioresponsiveness groups. However, using low-dose rate irradiation on primary human tumors with a soft agar clonogenic assay was not practical because of reducing the success rate for obtaining data for radiosensitivity measurements.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshCell Survival-
dc.subject.meshHumans-
dc.subject.meshModels, Biological-
dc.subject.meshRadiation Oncology-
dc.subject.meshRadiation Tolerance-
dc.subject.meshRadiotherapy Dosage-
dc.subject.meshTumor Cells, Cultured-
dc.titleDiscrimination of human tumor radioresponsiveness using low-dose rate irradiation.en
dc.typeArticleen
dc.contributor.departmentDepartment of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden.en
dc.identifier.journalInternational Journal of Radiation Oncology, Biology, Physicsen

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