Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?

2.50
Hdl Handle:
http://hdl.handle.net/10541/92344
Title:
Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?
Authors:
Scott, David; Barber, J B; Levine, Edward; Burrill, W; Roberts, Stephen A
Abstract:
Enhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lymphocytes irradiated in the G2 phase of the cell cycle. We suggested that, in addition to the highly penetrant genes BRCA1 and BRCA2, which confer a very high risk of breast cancer and are carried by about 5% of all breast cancer patients, there are also low-penetrance predisposing genes carried by a much higher proportion of breast cancer patients, a view supported by recent epidemiological studies. Ideally, testing for the presence of these putative genes should involve the use of simpler methods than the G2 assay, which requires metaphase analysis of chromosome damage. Here we report on the use of a simple, rapid micronucleus assay in G0 lymphocytes exposed to high dose rate (HDR) or low dose rate gamma-irradiation, with delayed mitogenic stimulation. Good assay reproducibility was obtained, particularly with the HDR protocol, which identified 31% (12 out of 39) of breast cancer patients compared with 5% (2 out of 42) of healthy controls as having elevated radiation sensitivity. In the long term, such cytogenetic assays may have the potential for selecting women for intensive screening for breast cancer.
Affiliation:
Paterson Institute for Cancer Research, Christie CRC Research Centre, Manchester, UK.
Citation:
Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition? 1998, 77 (4):614-20 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Feb-1998
URI:
http://hdl.handle.net/10541/92344
PubMed ID:
9484819
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorScott, Daviden
dc.contributor.authorBarber, J Ben
dc.contributor.authorLevine, Edwarden
dc.contributor.authorBurrill, Wen
dc.contributor.authorRoberts, Stephen Aen
dc.date.accessioned2010-02-17T12:00:50Z-
dc.date.available2010-02-17T12:00:50Z-
dc.date.issued1998-02-
dc.identifier.citationRadiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition? 1998, 77 (4):614-20 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid9484819-
dc.identifier.urihttp://hdl.handle.net/10541/92344-
dc.description.abstractEnhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lymphocytes irradiated in the G2 phase of the cell cycle. We suggested that, in addition to the highly penetrant genes BRCA1 and BRCA2, which confer a very high risk of breast cancer and are carried by about 5% of all breast cancer patients, there are also low-penetrance predisposing genes carried by a much higher proportion of breast cancer patients, a view supported by recent epidemiological studies. Ideally, testing for the presence of these putative genes should involve the use of simpler methods than the G2 assay, which requires metaphase analysis of chromosome damage. Here we report on the use of a simple, rapid micronucleus assay in G0 lymphocytes exposed to high dose rate (HDR) or low dose rate gamma-irradiation, with delayed mitogenic stimulation. Good assay reproducibility was obtained, particularly with the HDR protocol, which identified 31% (12 out of 39) of breast cancer patients compared with 5% (2 out of 42) of healthy controls as having elevated radiation sensitivity. In the long term, such cytogenetic assays may have the potential for selecting women for intensive screening for breast cancer.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshBreast Neoplasms-
dc.subject.meshCell Division-
dc.subject.meshConfounding Factors (Epidemiology)-
dc.subject.meshDisease Susceptibility-
dc.subject.meshFemale-
dc.subject.meshG0 Phase-
dc.subject.meshHumans-
dc.subject.meshLymphocytes-
dc.subject.meshMicronucleus Tests-
dc.subject.meshMiddle Aged-
dc.subject.meshRadiation Dosage-
dc.subject.meshReproducibility of Results-
dc.titleRadiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie CRC Research Centre, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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