Levels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92037
Title:
Levels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers.
Authors:
Herring, C J; West, Catharine M L; Wilks, Deepti P; Davidson, Susan E; Hunter, Robin D; Berry, P; Forster, Gill; MacKinnon, J; Rafferty, Joseph A; Elder, Rhoderick H; Hendry, Jolyon H; Margison, Geoffrey P
Abstract:
A study was made of the relationship between the intrinsic radiosensitivity of human cervical tumours and the expression of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (HAP1). The radiosensitivity of clonogenic cells in tumour biopsies was measured as surviving fraction at 2 Gy (SF2) using a soft agar assay. HAP1 expression levels were determined after staining of formalin-fixed paraffin-embedded tumour sections with a rabbit antiserum raised against recombinant HAP1. Both measurements were obtained on pretreatment biopsy material. All 25 tumours examined showed positive staining for HAP1, but there was heterogeneity in the level of expression both within and between tumours. The average coefficients of variation for intra- and intertumour heterogeneity were 62% and 82% respectively. There was a moderate but significant positive correlation between the levels of HAP1 expression and SF2 (r = 0.60, P = 0.002). Hence, this study shows that there is some relationship between intrinsic radiosensitivity and expression of a DNA repair enzyme in cervical carcinomas. The results suggest that this type of approach may be useful in the development of rapid predictive tests of tumour radiosensitivity.
Affiliation:
Cancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Manchester, UK.
Citation:
Levels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers. 1998, 78 (9):1128-33 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Nov-1998
URI:
http://hdl.handle.net/10541/92037
PubMed ID:
9820167
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorHerring, C Jen
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorWilks, Deepti Pen
dc.contributor.authorDavidson, Susan Een
dc.contributor.authorHunter, Robin Den
dc.contributor.authorBerry, Pen
dc.contributor.authorForster, Gillen
dc.contributor.authorMacKinnon, Jen
dc.contributor.authorRafferty, Joseph Aen
dc.contributor.authorElder, Rhoderick Hen
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorMargison, Geoffrey Pen
dc.date.accessioned2010-02-12T16:15:32Z-
dc.date.available2010-02-12T16:15:32Z-
dc.date.issued1998-11-
dc.identifier.citationLevels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers. 1998, 78 (9):1128-33 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid9820167-
dc.identifier.urihttp://hdl.handle.net/10541/92037-
dc.description.abstractA study was made of the relationship between the intrinsic radiosensitivity of human cervical tumours and the expression of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (HAP1). The radiosensitivity of clonogenic cells in tumour biopsies was measured as surviving fraction at 2 Gy (SF2) using a soft agar assay. HAP1 expression levels were determined after staining of formalin-fixed paraffin-embedded tumour sections with a rabbit antiserum raised against recombinant HAP1. Both measurements were obtained on pretreatment biopsy material. All 25 tumours examined showed positive staining for HAP1, but there was heterogeneity in the level of expression both within and between tumours. The average coefficients of variation for intra- and intertumour heterogeneity were 62% and 82% respectively. There was a moderate but significant positive correlation between the levels of HAP1 expression and SF2 (r = 0.60, P = 0.002). Hence, this study shows that there is some relationship between intrinsic radiosensitivity and expression of a DNA repair enzyme in cervical carcinomas. The results suggest that this type of approach may be useful in the development of rapid predictive tests of tumour radiosensitivity.en
dc.language.isoenen
dc.subjectUterine Cervical Canceren
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshAnimals-
dc.subject.meshCHO Cells-
dc.subject.meshCarbon-Oxygen Lyases-
dc.subject.meshCricetinae-
dc.subject.meshDNA Repair-
dc.subject.meshDNA-(Apurinic or Apyrimidinic Site) Lyase-
dc.subject.meshDeoxyribonuclease IV (Phage T4-Induced)-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshNuclear Proteins-
dc.subject.meshRabbits-
dc.subject.meshRadiation Tolerance-
dc.subject.meshRats-
dc.subject.meshTumor Suppressor Protein p53-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleLevels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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