Factors which affect the CFU-GM content of the peripheral blood haemopoietic progenitor cell harvests in patients with acute myeloid leukaemia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92035
Title:
Factors which affect the CFU-GM content of the peripheral blood haemopoietic progenitor cell harvests in patients with acute myeloid leukaemia.
Authors:
Jowitt, Simon N; Chang, James; Morgenstern, Godfrey R; Howe, T; Ryder, W David J; Testa, Nydia G; Scarffe, J Howard
Abstract:
Autologous peripheral blood haemopoietic stem cells (PBSC) were harvested from 30 patients with de novo acute leukaemia, 29 of whom had entered remission following standard chemotherapy. Correlation of CD34+ cells/kg to CFU-GM/kg in the harvests was good (correlation coefficient = 0.72, P < 0.001). We demonstrated significant associations between the CFU-GM content of the harvest and the following: time to platelets >50 x 10(9)/l post final induction course (P < 0.001), days to harvest from day 1 of intensification/mobilization (correlation coefficient = -0.73, P < 0.001), platelets >20 x 10(9)/l at time of harvest (P = 0.02), time to WBC >1.0 x 10(9)/l post intensification/mobilization (correlation coefficient = -0.70, P < 0.001), and WBC on day of harvest (correlation coefficient = 0.60, P < 0.001). In contrast, we found no relationship between the CFU-GM content of the harvest and patient age up to 65 years, presence of absence of coexistent features of trilineage myelodysplasia at diagnosis, number of induction courses to remission or total number of courses of chemotherapy prior to intensification/mobilization. Haemopoietic recovery after reinfusion of PBSC was highly correlated to the number of CFU-GM infused (neutrophils >0.5 x 10(9)/l rs = -0.72, P = 0.001; platelets >20 x 10(9)/l unsupported rs = -0.71, P = 0.001). Our results show that the number of induction courses received, and thus exposure to cytotoxic agents received, made no significant difference to subsequent CFU-GM harvest content. We collected superior harvests from those patients with faster platelet recovery following mobilization therapy. We also found that faster platelet recovery following the final induction therapy was a better predictor of the CFU-GM harvest following mobilization than was the neutrophil recovery following final induction.
Affiliation:
Department of Haematological Oncology, Christie Hospital NHS Trust, Manchester.
Citation:
Factors which affect the CFU-GM content of the peripheral blood haemopoietic progenitor cell harvests in patients with acute myeloid leukaemia. 1998, 100 (4):688-94 Br. J. Haematol.
Journal:
British Journal of Haematology
Issue Date:
Mar-1998
URI:
http://hdl.handle.net/10541/92035
PubMed ID:
9531335
Type:
Article
Language:
en
ISSN:
0007-1048
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorJowitt, Simon Nen
dc.contributor.authorChang, Jamesen
dc.contributor.authorMorgenstern, Godfrey Ren
dc.contributor.authorHowe, Ten
dc.contributor.authorRyder, W David Jen
dc.contributor.authorTesta, Nydia Gen
dc.contributor.authorScarffe, J Howarden
dc.date.accessioned2010-02-12T16:12:34Z-
dc.date.available2010-02-12T16:12:34Z-
dc.date.issued1998-03-
dc.identifier.citationFactors which affect the CFU-GM content of the peripheral blood haemopoietic progenitor cell harvests in patients with acute myeloid leukaemia. 1998, 100 (4):688-94 Br. J. Haematol.en
dc.identifier.issn0007-1048-
dc.identifier.pmid9531335-
dc.identifier.urihttp://hdl.handle.net/10541/92035-
dc.description.abstractAutologous peripheral blood haemopoietic stem cells (PBSC) were harvested from 30 patients with de novo acute leukaemia, 29 of whom had entered remission following standard chemotherapy. Correlation of CD34+ cells/kg to CFU-GM/kg in the harvests was good (correlation coefficient = 0.72, P < 0.001). We demonstrated significant associations between the CFU-GM content of the harvest and the following: time to platelets >50 x 10(9)/l post final induction course (P < 0.001), days to harvest from day 1 of intensification/mobilization (correlation coefficient = -0.73, P < 0.001), platelets >20 x 10(9)/l at time of harvest (P = 0.02), time to WBC >1.0 x 10(9)/l post intensification/mobilization (correlation coefficient = -0.70, P < 0.001), and WBC on day of harvest (correlation coefficient = 0.60, P < 0.001). In contrast, we found no relationship between the CFU-GM content of the harvest and patient age up to 65 years, presence of absence of coexistent features of trilineage myelodysplasia at diagnosis, number of induction courses to remission or total number of courses of chemotherapy prior to intensification/mobilization. Haemopoietic recovery after reinfusion of PBSC was highly correlated to the number of CFU-GM infused (neutrophils >0.5 x 10(9)/l rs = -0.72, P = 0.001; platelets >20 x 10(9)/l unsupported rs = -0.71, P = 0.001). Our results show that the number of induction courses received, and thus exposure to cytotoxic agents received, made no significant difference to subsequent CFU-GM harvest content. We collected superior harvests from those patients with faster platelet recovery following mobilization therapy. We also found that faster platelet recovery following the final induction therapy was a better predictor of the CFU-GM harvest following mobilization than was the neutrophil recovery following final induction.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cell Mobilisationen
dc.subjectHaematopoietic Stem Cell Transplantationen
dc.subjectHaematopoietic Stem Cellsen
dc.subjectMyeloid Leukaemiaen
dc.subject.meshAcute Disease-
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAntineoplastic Agents, Alkylating-
dc.subject.meshBusulfan-
dc.subject.meshCyclophosphamide-
dc.subject.meshFemale-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshHematopoietic Stem Cell Mobilization-
dc.subject.meshHematopoietic Stem Cell Transplantation-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshLeukemia, Myeloid-
dc.subject.meshLeukocyte Count-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshNeutrophils-
dc.subject.meshPlatelet Count-
dc.subject.meshTretinoin-
dc.titleFactors which affect the CFU-GM content of the peripheral blood haemopoietic progenitor cell harvests in patients with acute myeloid leukaemia.en
dc.typeArticleen
dc.contributor.departmentDepartment of Haematological Oncology, Christie Hospital NHS Trust, Manchester.en
dc.identifier.journalBritish Journal of Haematologyen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.