Radiation-induced micronuclei in human fibroblasts in relation to clonogenic radiosensitivity.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92032
Title:
Radiation-induced micronuclei in human fibroblasts in relation to clonogenic radiosensitivity.
Authors:
O'Driscoll, M C; Scott, David; Orton, C J; Kiltie, Anne E; Davidson, Susan E; Hunter, Robin D; West, Catharine M L
Abstract:
As part of our programme for developing predictive tests for normal tissue response to radiotherapy, we have investigated the efficacy of the cytokinesis-block micronucleus (MN) assay as a means of detecting interindividual differences in cellular radiosensitivity. A study was made of nine fibroblast strains established from vaginal biopsies of pretreatment cervical cancer patients and an ataxia telangiectasia (A-T) cell strain. Cells were irradiated in plateau phase, replated and treated with cytochalasin B 24 h later. MN formation was examined 72 h after irradiation as the number of MN in 100 binucleate cells. The method yielded low spontaneous MN yields (<7 per 100 cells), and mean induced MN frequencies after 3.5 Gy varied between cell strains from 18 to 144 per 100 cells. However, in repeat experiments, considerable intrastrain variability was observed (CV = 32%), with up to twofold differences in MN yields, although this was less than interstrain variability (CV = 62%). An analysis was made of the relationship between MN results and previously obtained clonogenic survival data. There was a significant correlation between MN yields and clonogenic survival. However, when the A-T strain was excluded from the analysis, the correlation lost significance, mainly because of one slow-growing strain which was the most sensitive to cell killing but had almost the lowest MN frequency. With current methodology, the MN assay on human fibroblasts does not appear to have a role in predictive testing of normal tissue radiosensitivity.
Affiliation:
Cancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
Radiation-induced micronuclei in human fibroblasts in relation to clonogenic radiosensitivity. 1998, 78 (12):1559-63 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Dec-1998
URI:
http://hdl.handle.net/10541/92032
PubMed ID:
9862564
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Driscoll, M Cen
dc.contributor.authorScott, Daviden
dc.contributor.authorOrton, C Jen
dc.contributor.authorKiltie, Anne Een
dc.contributor.authorDavidson, Susan Een
dc.contributor.authorHunter, Robin Den
dc.contributor.authorWest, Catharine M Len
dc.date.accessioned2010-02-12T15:59:21Z-
dc.date.available2010-02-12T15:59:21Z-
dc.date.issued1998-12-
dc.identifier.citationRadiation-induced micronuclei in human fibroblasts in relation to clonogenic radiosensitivity. 1998, 78 (12):1559-63 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid9862564-
dc.identifier.urihttp://hdl.handle.net/10541/92032-
dc.description.abstractAs part of our programme for developing predictive tests for normal tissue response to radiotherapy, we have investigated the efficacy of the cytokinesis-block micronucleus (MN) assay as a means of detecting interindividual differences in cellular radiosensitivity. A study was made of nine fibroblast strains established from vaginal biopsies of pretreatment cervical cancer patients and an ataxia telangiectasia (A-T) cell strain. Cells were irradiated in plateau phase, replated and treated with cytochalasin B 24 h later. MN formation was examined 72 h after irradiation as the number of MN in 100 binucleate cells. The method yielded low spontaneous MN yields (<7 per 100 cells), and mean induced MN frequencies after 3.5 Gy varied between cell strains from 18 to 144 per 100 cells. However, in repeat experiments, considerable intrastrain variability was observed (CV = 32%), with up to twofold differences in MN yields, although this was less than interstrain variability (CV = 62%). An analysis was made of the relationship between MN results and previously obtained clonogenic survival data. There was a significant correlation between MN yields and clonogenic survival. However, when the A-T strain was excluded from the analysis, the correlation lost significance, mainly because of one slow-growing strain which was the most sensitive to cell killing but had almost the lowest MN frequency. With current methodology, the MN assay on human fibroblasts does not appear to have a role in predictive testing of normal tissue radiosensitivity.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshCell Survival-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshFibroblasts-
dc.subject.meshHumans-
dc.subject.meshMicronucleus Tests-
dc.subject.meshRadiation Tolerance-
dc.subject.meshTumor Cells, Cultured-
dc.titleRadiation-induced micronuclei in human fibroblasts in relation to clonogenic radiosensitivity.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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