Interaction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma.

2.50
Hdl Handle:
http://hdl.handle.net/10541/92031
Title:
Interaction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma.
Authors:
Lang, Shona H; Clarke, Noel W ( 0000-0001-7776-8059 ) ; George, N J; Allen, Terence D; Testa, Nydia G
Abstract:
BACKGROUND: Metastases of prostate cancer form selectively within the skeleton. To understand this metastatic spread, we studied the ability of prostate epithelial cells to grow and proliferate within the bone marrow, using primary coculture. METHODS: Prostate epithelia and fibroblasts were prepared from men with benign prostatic hyperplasia (n = 13) and cancer of the prostate (n = 10). Confluent cultures of bone-marrow stroma or fibroblast controls were prepared in 96-well plates, and identical plates were treated with detergent to expose the extracellular matrix of the cells. Epithelial cells were seeded onto either cells or matrix, and their growth characteristics were determined by counting increases in colony size and number over time. Further experiments evaluated the effects on epithelial growth when cells were exposed to media conditioned by these stroma, using an MTT assay. RESULTS: Results showed that for epithelial cells derived from malignant (or benign) tissue, the median value of the total area of colonies formed on bone-marrow stroma was 2.1 (benign, 2.6) mm2, in contrast to 0.3 (benign, 0.4) mm2 or 0.25 (benign, 0) mm2 when these cells were cocultured with fibroblasts from benign or malignant prostates, respectively. Statistics indicated that growth was significantly greater on bone-marrow stroma than on control stroma (P < 0.005). However, no significant stimulation of epithelial cell growth was seen when these epithelial cells were cultured on extracellular matrix from bone-marrow stroma or when exposed to bone-marrow stroma-conditioned media in comparison to fibroblast controls. No statistical differences were found between the formation of colonies from malignant tissue in comparison to benign. CONCLUSIONS: This system allows the investigation of bone-marrow stroma colonization by primary prostate epithelial cells, and could be developed for the study of metastasis.
Affiliation:
Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital National Health Service Trust, Manchester, UK. shl2@york.ac.uk
Citation:
Interaction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma. 1998, 34 (3):203-13 Prostate
Journal:
Prostate
Issue Date:
15-Feb-1998
URI:
http://hdl.handle.net/10541/92031
DOI:
10.1002/(SICI)1097-0045(19980215)34:3<203::AID-PROS8>3.0.CO;2-I
PubMed ID:
9492849
Type:
Article
Language:
en
ISSN:
0270-4137
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLang, Shona Hen
dc.contributor.authorClarke, Noel Wen
dc.contributor.authorGeorge, N Jen
dc.contributor.authorAllen, Terence Den
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-02-12T15:54:24Z-
dc.date.available2010-02-12T15:54:24Z-
dc.date.issued1998-02-15-
dc.identifier.citationInteraction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma. 1998, 34 (3):203-13 Prostateen
dc.identifier.issn0270-4137-
dc.identifier.pmid9492849-
dc.identifier.doi10.1002/(SICI)1097-0045(19980215)34:3<203::AID-PROS8>3.0.CO;2-I-
dc.identifier.urihttp://hdl.handle.net/10541/92031-
dc.description.abstractBACKGROUND: Metastases of prostate cancer form selectively within the skeleton. To understand this metastatic spread, we studied the ability of prostate epithelial cells to grow and proliferate within the bone marrow, using primary coculture. METHODS: Prostate epithelia and fibroblasts were prepared from men with benign prostatic hyperplasia (n = 13) and cancer of the prostate (n = 10). Confluent cultures of bone-marrow stroma or fibroblast controls were prepared in 96-well plates, and identical plates were treated with detergent to expose the extracellular matrix of the cells. Epithelial cells were seeded onto either cells or matrix, and their growth characteristics were determined by counting increases in colony size and number over time. Further experiments evaluated the effects on epithelial growth when cells were exposed to media conditioned by these stroma, using an MTT assay. RESULTS: Results showed that for epithelial cells derived from malignant (or benign) tissue, the median value of the total area of colonies formed on bone-marrow stroma was 2.1 (benign, 2.6) mm2, in contrast to 0.3 (benign, 0.4) mm2 or 0.25 (benign, 0) mm2 when these cells were cocultured with fibroblasts from benign or malignant prostates, respectively. Statistics indicated that growth was significantly greater on bone-marrow stroma than on control stroma (P < 0.005). However, no significant stimulation of epithelial cell growth was seen when these epithelial cells were cultured on extracellular matrix from bone-marrow stroma or when exposed to bone-marrow stroma-conditioned media in comparison to fibroblast controls. No statistical differences were found between the formation of colonies from malignant tissue in comparison to benign. CONCLUSIONS: This system allows the investigation of bone-marrow stroma colonization by primary prostate epithelial cells, and could be developed for the study of metastasis.en
dc.language.isoenen
dc.subjectCancer Metastasisen
dc.subjectProstatic Canceren
dc.subjectCultured Tumour Cellsen
dc.subject.meshBone Marrow Cells-
dc.subject.meshCell Communication-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshCoculture Techniques-
dc.subject.meshCulture Media, Conditioned-
dc.subject.meshEpithelial Cells-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshNeoplasm Metastasis-
dc.subject.meshProstate-
dc.subject.meshProstatic Hyperplasia-
dc.subject.meshProstatic Neoplasms-
dc.subject.meshStromal Cells-
dc.subject.meshTime Factors-
dc.subject.meshTumor Cells, Cultured-
dc.titleInteraction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital National Health Service Trust, Manchester, UK. shl2@york.ac.uken
dc.identifier.journalProstateen

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