PCR-ELISA for the early diagnosis of invasive pulmonary aspergillus infection in neutropenic patients.

2.50
Hdl Handle:
http://hdl.handle.net/10541/91989
Title:
PCR-ELISA for the early diagnosis of invasive pulmonary aspergillus infection in neutropenic patients.
Authors:
Jones, M E; Fox, A J; Barnes, A J; Oppenheim, B A; Balagopal, P; Morgenstern, Godfrey R; Scarffe, J Howard
Abstract:
AIM: To evaluate a newly developed aspergillus mitochondrial gene PCR-ELISA assay for the early diagnosis of invasive pulmonary aspergillosis (IPA) in neutropenic patients. METHODS: The aspergillus mitochondrial gene was chosen for the amplification target for use with a solution hybridisation assay with colorimetric end stage detection in microtitre plate format (PCR-ELISA). The study group comprised neutropenic patients undergoing febrile episodes not responding to standard antibacterial antibiotics. Patients underwent computed tomography and bronchoscopy. Bronchoalveolar lavage (BAL) fluids were examined by culture and PCR. RESULTS: The aspergillus mitochondrial gene PCR-ELISA was both sensitive (100%) and specific (100%) for IPA in neutropenic patients. All 12 patients with definite or probable IPA had PCR positive BAL fluids. None of the patients with undiagnosed or confirmed infections of other aetiologies were mitochondrial PCR positive. Speciation based upon amplicon size difference was possible. CONCLUSIONS: Aspergillus mitochondrial DNA PCR-ELISA on BAL fluid is useful in the early diagnosis of IPA in neutropenic patients alone or, potentially, as an indication for thoracic computed tomography.
Affiliation:
Manchester Public Health Laboratory, Withington Hospital, UK.
Citation:
PCR-ELISA for the early diagnosis of invasive pulmonary aspergillus infection in neutropenic patients. 1998, 51 (9):652-6 J. Clin. Pathol.
Journal:
Journal of Clinical Pathology
Issue Date:
Sep-1998
URI:
http://hdl.handle.net/10541/91989
PubMed ID:
9930067
Type:
Article
Language:
en
ISSN:
0021-9746
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorJones, M Een
dc.contributor.authorFox, A Jen
dc.contributor.authorBarnes, A Jen
dc.contributor.authorOppenheim, B Aen
dc.contributor.authorBalagopal, Pen
dc.contributor.authorMorgenstern, Godfrey Ren
dc.contributor.authorScarffe, J Howarden
dc.date.accessioned2010-02-12T15:00:16Z-
dc.date.available2010-02-12T15:00:16Z-
dc.date.issued1998-09-
dc.identifier.citationPCR-ELISA for the early diagnosis of invasive pulmonary aspergillus infection in neutropenic patients. 1998, 51 (9):652-6 J. Clin. Pathol.en
dc.identifier.issn0021-9746-
dc.identifier.pmid9930067-
dc.identifier.urihttp://hdl.handle.net/10541/91989-
dc.description.abstractAIM: To evaluate a newly developed aspergillus mitochondrial gene PCR-ELISA assay for the early diagnosis of invasive pulmonary aspergillosis (IPA) in neutropenic patients. METHODS: The aspergillus mitochondrial gene was chosen for the amplification target for use with a solution hybridisation assay with colorimetric end stage detection in microtitre plate format (PCR-ELISA). The study group comprised neutropenic patients undergoing febrile episodes not responding to standard antibacterial antibiotics. Patients underwent computed tomography and bronchoscopy. Bronchoalveolar lavage (BAL) fluids were examined by culture and PCR. RESULTS: The aspergillus mitochondrial gene PCR-ELISA was both sensitive (100%) and specific (100%) for IPA in neutropenic patients. All 12 patients with definite or probable IPA had PCR positive BAL fluids. None of the patients with undiagnosed or confirmed infections of other aetiologies were mitochondrial PCR positive. Speciation based upon amplicon size difference was possible. CONCLUSIONS: Aspergillus mitochondrial DNA PCR-ELISA on BAL fluid is useful in the early diagnosis of IPA in neutropenic patients alone or, potentially, as an indication for thoracic computed tomography.en
dc.language.isoenen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAspergillosis-
dc.subject.meshBronchoalveolar Lavage Fluid-
dc.subject.meshDNA, Fungal-
dc.subject.meshDNA, Mitochondrial-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshImmunocompromised Host-
dc.subject.meshLung Diseases, Fungal-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshNeutropenia-
dc.subject.meshOpportunistic Infections-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshTime Factors-
dc.titlePCR-ELISA for the early diagnosis of invasive pulmonary aspergillus infection in neutropenic patients.en
dc.typeArticleen
dc.contributor.departmentManchester Public Health Laboratory, Withington Hospital, UK.en
dc.identifier.journalJournal of Clinical Pathologyen
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