Serum insulin-like growth factor-I, IGF binding protein-3 and IGFBP-3 protease activity after cranial irradiation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/91674
Title:
Serum insulin-like growth factor-I, IGF binding protein-3 and IGFBP-3 protease activity after cranial irradiation.
Authors:
Tillmann, Vallo; Shalet, Stephen M; Price, David A; Wales, J K H; Pennells, Louise; Soden, Joanne; Gill, Matthew S; Whatmore, Andrew J; Clayton, Peter E
Abstract:
The relationship between peak growth hormone (GH), insulin-like growth factor I (IGF-I), IGF-I binding protein 3 (IGFBP-3) and IGFBP-3 protease activity was studied in 28 children and adolescents undergoing investigation of pituitary function 0.4-14.2 years after cranial or craniospinal irradiation for the treatment of CNS tumours distant from the hypothalamic-pituitary axis (n = 16) or prophylaxis against CNS leukaemia (n = 12). Seven out of 15 patients with GH deficiency (GHD) (defined as a peak GH concentration <7.5 ng/ml in a stimulation test) had IGF-I <-2 standard deviation score (SDS). None of the 28 patients had serum IGFBP-3 concentrations measured by radioimmunoassay (RIA) <-1.5 SDS with no difference between those with and without GHD. IGFBP-3 concentrations measured by RIA were strongly correlated to IGFBP-3 band density on Western ligand blot (WLB) (r = 0.71; p < 0.0001). IGFBP-3 protease activity was negatively correlated to IGFBP-3 by RIA (r = -0.55; p < 0.01) and to IGFBP-3 by WLB (r = -0.51; p < 0.01). Twenty-two patients had normal IGFBP-3 protease activity (<30% of the activity in pregnancy serum) indicating that serum IGFBP-3 protease activity does not account for the normal levels of IGFBP-3 in RIA. Low serum IGF-I but normal IGFBP-3 concentrations and in the majority normal IGFBP-3 protease activity was found in patients in the years after CNS irradiation. Neither serum IGF-I nor IGFBP-3 can be used as a reliable index of the development of radiation-induced GHD.
Affiliation:
Royal Manchester Children's Hospital, Manchester, UK.
Citation:
Serum insulin-like growth factor-I, IGF binding protein-3 and IGFBP-3 protease activity after cranial irradiation. 1998, 50 (2):71-7 Horm. Res.
Journal:
Hormone Research
Issue Date:
1998
URI:
http://hdl.handle.net/10541/91674
DOI:
10.1159/000023237
PubMed ID:
9701699
Type:
Article
Language:
en
ISSN:
0301-0163
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorTillmann, Valloen
dc.contributor.authorShalet, Stephen Men
dc.contributor.authorPrice, David Aen
dc.contributor.authorWales, J K Hen
dc.contributor.authorPennells, Louiseen
dc.contributor.authorSoden, Joanneen
dc.contributor.authorGill, Matthew Sen
dc.contributor.authorWhatmore, Andrew Jen
dc.contributor.authorClayton, Peter Een
dc.date.accessioned2010-02-09T16:47:02Z-
dc.date.available2010-02-09T16:47:02Z-
dc.date.issued1998-
dc.identifier.citationSerum insulin-like growth factor-I, IGF binding protein-3 and IGFBP-3 protease activity after cranial irradiation. 1998, 50 (2):71-7 Horm. Res.en
dc.identifier.issn0301-0163-
dc.identifier.pmid9701699-
dc.identifier.doi10.1159/000023237-
dc.identifier.urihttp://hdl.handle.net/10541/91674-
dc.description.abstractThe relationship between peak growth hormone (GH), insulin-like growth factor I (IGF-I), IGF-I binding protein 3 (IGFBP-3) and IGFBP-3 protease activity was studied in 28 children and adolescents undergoing investigation of pituitary function 0.4-14.2 years after cranial or craniospinal irradiation for the treatment of CNS tumours distant from the hypothalamic-pituitary axis (n = 16) or prophylaxis against CNS leukaemia (n = 12). Seven out of 15 patients with GH deficiency (GHD) (defined as a peak GH concentration <7.5 ng/ml in a stimulation test) had IGF-I <-2 standard deviation score (SDS). None of the 28 patients had serum IGFBP-3 concentrations measured by radioimmunoassay (RIA) <-1.5 SDS with no difference between those with and without GHD. IGFBP-3 concentrations measured by RIA were strongly correlated to IGFBP-3 band density on Western ligand blot (WLB) (r = 0.71; p < 0.0001). IGFBP-3 protease activity was negatively correlated to IGFBP-3 by RIA (r = -0.55; p < 0.01) and to IGFBP-3 by WLB (r = -0.51; p < 0.01). Twenty-two patients had normal IGFBP-3 protease activity (<30% of the activity in pregnancy serum) indicating that serum IGFBP-3 protease activity does not account for the normal levels of IGFBP-3 in RIA. Low serum IGF-I but normal IGFBP-3 concentrations and in the majority normal IGFBP-3 protease activity was found in patients in the years after CNS irradiation. Neither serum IGF-I nor IGFBP-3 can be used as a reliable index of the development of radiation-induced GHD.en
dc.language.isoenen
dc.subjectLeukaemiaen
dc.subjectCentral Nervous System Canceren
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshBody Height-
dc.subject.meshCentral Nervous System Neoplasms-
dc.subject.meshChild-
dc.subject.meshEndopeptidases-
dc.subject.meshFemale-
dc.subject.meshGrowth Disorders-
dc.subject.meshHuman Growth Hormone-
dc.subject.meshHumans-
dc.subject.meshInsulin-Like Growth Factor Binding Protein 3-
dc.subject.meshInsulin-Like Growth Factor I-
dc.subject.meshLeukemia-
dc.subject.meshMale-
dc.subject.meshRadiotherapy-
dc.titleSerum insulin-like growth factor-I, IGF binding protein-3 and IGFBP-3 protease activity after cranial irradiation.en
dc.typeArticleen
dc.contributor.departmentRoyal Manchester Children's Hospital, Manchester, UK.en
dc.identifier.journalHormone Researchen
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