Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/91628
Title:
Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation.
Authors:
Doyle, Alex; Martín-García, Rebeca; Coulton, Arthur T; Bagley, Steven; Mulvihill, Daniel P
Abstract:
Class V myosins are dimeric actin-associated motor proteins that deliver cellular cargoes to discrete cellular locations. Fission yeast possess two class V myosins, Myo51 and Myo52. Although Myo52 has been shown to have roles in vacuole distribution, cytokinesis and cell growth, Myo51 has no as yet discernible function in the vegetative life cycle. Here, we uncover distinct functions for this motor protein during mating and meiosis. Not only does Myo51 transiently localise to a foci at the site of cell fusion upon conjugation, but overexpression of the Myo51 globular tail also leads to disruption of cell fusion. Upon completion of meiotic prophase Myo51 localises to the outside of the spindle pole bodies (SPBs), where it remains until completion of meiosis II. Association of Myo51 with SPBs is not dependent upon actin or the septation initiation network (SIN); however, it is dependent on a stable microtubule cytoskeleton and the presence of the Cdc2-CyclinB complex. We observe a rapid and dynamic exchange of Myo51 at the SPB during meiosis I but not meiosis II. Finally, we show that Myo51 has an important role in regulating spore formation upon completion of meiosis.
Affiliation:
School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK.
Citation:
Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation. 2009, 122 (Pt 23):4330-40 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
1-Dec-2009
URI:
http://hdl.handle.net/10541/91628
DOI:
10.1242/jcs.055202
PubMed ID:
19887589
Type:
Article
Language:
en
ISSN:
1477-9137
Appears in Collections:
All Paterson Institute for Cancer Research; Advanced Imaging

Full metadata record

DC FieldValue Language
dc.contributor.authorDoyle, Alexen
dc.contributor.authorMartín-García, Rebecaen
dc.contributor.authorCoulton, Arthur Ten
dc.contributor.authorBagley, Stevenen
dc.contributor.authorMulvihill, Daniel Pen
dc.date.accessioned2010-02-09T14:55:15Z-
dc.date.available2010-02-09T14:55:15Z-
dc.date.issued2009-12-01-
dc.identifier.citationFission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation. 2009, 122 (Pt 23):4330-40 J. Cell. Sci.en
dc.identifier.issn1477-9137-
dc.identifier.pmid19887589-
dc.identifier.doi10.1242/jcs.055202-
dc.identifier.urihttp://hdl.handle.net/10541/91628-
dc.description.abstractClass V myosins are dimeric actin-associated motor proteins that deliver cellular cargoes to discrete cellular locations. Fission yeast possess two class V myosins, Myo51 and Myo52. Although Myo52 has been shown to have roles in vacuole distribution, cytokinesis and cell growth, Myo51 has no as yet discernible function in the vegetative life cycle. Here, we uncover distinct functions for this motor protein during mating and meiosis. Not only does Myo51 transiently localise to a foci at the site of cell fusion upon conjugation, but overexpression of the Myo51 globular tail also leads to disruption of cell fusion. Upon completion of meiotic prophase Myo51 localises to the outside of the spindle pole bodies (SPBs), where it remains until completion of meiosis II. Association of Myo51 with SPBs is not dependent upon actin or the septation initiation network (SIN); however, it is dependent on a stable microtubule cytoskeleton and the presence of the Cdc2-CyclinB complex. We observe a rapid and dynamic exchange of Myo51 at the SPB during meiosis I but not meiosis II. Finally, we show that Myo51 has an important role in regulating spore formation upon completion of meiosis.en
dc.language.isoenen
dc.subject.meshCell Cycle-
dc.subject.meshMeiosis-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshMyosin Type V-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces pombe Proteins-
dc.subject.meshSpores, Fungal-
dc.titleFission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation.en
dc.typeArticleen
dc.contributor.departmentSchool of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK.en
dc.identifier.journalJournal of Cell Scienceen

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